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Composition for calming nerves containing exopolysaccharide produced by cereus cerevisiae as an active ingredient

A technology for tearing Cerobacteria and exopolysaccharides, which is applied in the functions of food ingredients, microorganisms, food ingredients, etc., can solve problems such as insufficient research on industrialization

Active Publication Date: 2021-06-04
福健生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fact is that since the first report of cereus tearing in the academic circle in 2002, there are still insufficient researches on its industrialization

Method used

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  • Composition for calming nerves containing exopolysaccharide produced by cereus cerevisiae as an active ingredient
  • Composition for calming nerves containing exopolysaccharide produced by cereus cerevisiae as an active ingredient
  • Composition for calming nerves containing exopolysaccharide produced by cereus cerevisiae as an active ingredient

Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0051] Production Example 1. Production of the culture solution of Ceruleus lamina, its dry powder, extract, and exopolysaccharide (hereinafter referred to as "EPS")

[0052] 1.1 Manufacture of the culture solution of cereus torn fungus

[0053] The mucormyces isolated from living oak trees collected in Sangju, Gyeongsangbuk-do were subcultured to cultivate mucormyces, which were stored frozen at -80°C, and the preserved strains were stored in PDA (Potato dextrose agar ) medium (87 plastic medium, medium (Difco), Becton, Dickinson and Company (Becton, Dickinson and Company)) subcultured 2 to 3 times, and the strain (hereinafter referred to as "PDA culture strain") was preserved Use in a refrigerator at 4°C. And after 600ml of PDB (Potato dextrose broth) medium (Difco, Becton Dickinson and Company) was composed in the conical flask, a PDA culture strain was placed here and shaken at 25°C for 8 days to obtain the PDB culture strain .

[0054] Afterwards, the liquid culture ...

Embodiment 1

[0061] Example 1. Characteristic evaluation of EPS

[0062] 1.1 Molecules of EPS by Gel Permeation Chromatography (GPC) Quantitative determination

[0063] The EPS produced in Production Example 1 was dissolved in 0.1M Na 2 SO 4 / 0.05MNaN 3 (The pH was adjusted to 4 with glacial acetic acid) After the solution was centrifuged at 4,000 rpm for 0.5 hours, only the supernatant was filtered through a 0.45 μm syringe filter, and analyzed by GPC for analysis.

[0064] Specifically, in terms of GPC analysis conditions, the refractive index is used by the detector, in terms of the GPC column, OHpak SB 805 HQ (Shodex, Japan) is used, and in terms of the mobile phase, 0.1M Na 2 SO 4 / 0.05M NaN 3 (Adjust the pH to 4 with glacial acetic acid), and the flow rate of the mobile phase flows in at 1.0ml / min. The standard curve was made using dextran (American Polymer Corporation, USA) with different molecular weights (130kDa, 400kDa, 770kDa, or 1200kDa), and the refractive index (re...

Embodiment 2

[0079] Embodiment 2. Verification of the nerve calming effect of EPS

[0080] In order to investigate the nerve-sedating effect of EPS isolated from the mycelia culture solution of R. cerevisiae, hippocampal single cells isolated from embryonic hippocampal nerve tissues of white mice were treated with the EPS produced in the above Production Example 1. , to measure changes in intracellular calcium concentration. Specifically, hippocampal nerve tissue was isolated from embryos of white mice on day 18 to day 19 of pregnancy under a dissecting microscope, added with 0.25% trypsin, and allowed to react at 37° C. for 10 minutes. Next, the tissue was washed several times with HBSS (Hanks' Balanced Salt Solution) to remove trypsin, and the hippocampal cells were separated into single cells with a 1 ml pipette. After the isolated hippocampal single cells were treated with the EPS produced in Production Example 1 at a concentration of 100 ug / mL, it was described in the literature (Dun...

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Abstract

The present invention relates to a composition for calming nerves. The composition for calming nerves contains the following active ingredients: exopolysaccharide produced by Ceriporia lacerata; The mycelium culture fluid of Ceruleus lacerator; the dry powder of the mycelium culture fluid; or the extract of the mycelium culture fluid, the composition can be used for preventing or treating depression or anxiety Tranquilizer or health functional food with nerve calming effect.

Description

technical field [0001] The present invention relates to a composition for calming nerves, which contains the following active ingredients: exopolysaccharide produced by Ceriporia lacerata or laceration containing exopolysaccharide The mycelium culture fluid, the dry powder or the extract of the mycelia culture fluid. Background technique [0002] The recent trend is that there is a sharp increase in patients with depression or anxiety due to various reasons such as economic problems such as economic growth rate, divorce and children's problems. Anxiety is defined as a largely unpleasant and unconscious feeling of anxiety, with associated physical symptoms (palpitations, sticky sweat, etc.) and behavioral symptoms (anaphylaxis, wandering, etc.) (Robert F .Scmidt, Human physiology, pp.366; Min Sunggil, Current Psychiatry, pp.238-40; Argyropoulos S.V. et al., Pharmacol.Ther.88, pp.213-27, 2000 ). When anxious, the whole brain enters an arousal state, and as a result, periphe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A23L31/00
CPCA23V2002/00A61K36/06A23V2200/31A23L31/00A23L33/10A23L33/17Y10S435/853
Inventor 金润秀
Owner 福健生物制药有限公司
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