A kind of multiple PCR kit and detection method for transgenic soybean detection

Abstract

The invention discloses a multiplex PCR kit and detection method for transgenic soybean detection, which can quickly detect 12 kinds of transgenic soybeans approved for import by the Ministry of Agriculture of my country, including MON87701, GTS40-3-2, MON89788, CV127, A2704, A5547, DP356043 , DP305423, MON87769, MON87708, 305423×40‑3‑2, MON87701×MON89788; the multiplex PCR kit disclosed by the present invention is based on the multiplex PCR method mediated by “public primers”, including public primers, multiplex primers, and multiplex PCR system detection The two-step annealing temperature method improves the specificity and sensitivity of the multiplex PCR system. The multiplex PCR method is inclusive and can continue to increase detection targets, which is conducive to improving the detection throughput of multiplex PCR. The invention has the advantages of high sensitivity, economy, speed, simple operation and the like, and can be applied to food detection.

Description

technical field [0001] The invention belongs to the field of food / gene detection, and in particular relates to a multiple PCR kit and a detection method for transgenic soybean detection. Background technique [0002] At present, the detection technologies of genetically modified crops reported at home and abroad are mainly divided into three categories, one is nucleic acid-based detection technology; the other is protein-based enzymatic and immunological detection technology; Biosensors and biochips for technological development, near-infrared spectroscopy and mass spectrometry analysis techniques based on modern analytical instruments, etc. The second type of protein-based detection technology is to detect proteins expressed by exogenous genes in transgenic plants. These proteins are easily damaged and inactivated, decomposed or disappeared during processing. The results are prone to false negatives and the method has poor repeatability. New technologies for genetically mo...

AI Technical Summary

Problems solved by technology

The second type of protein-based detection technology is to detect proteins expressed by exogenous genes in transgenic plants. These proteins are easily damaged and inactivated, decomposed or disappear during processing, and the inspection results are prone to false negatives and the repeatability of the method is poor.

New technologies for genetically modified detection developed in recent years, such as biosensors, chromatography, and near-infrared spectroscopy, are still in the research stage, and there are some shortcomings that cannot be effectively solved for the time being or exist objectively. For example, chromatography is only applicable to genetically modified Qualitative detection and analysis when major changes occur in the composition of plants and their products, this type of detection technology cannot be effectively promoted and applied in the short term

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