Multiple PCR kit for detecting transgenic soybeans and detection method

Abstract

The invention discloses a multiple PCR kit for detecting transgenic soybeans and a detection method. The multiple PCR kit for detecting transgenic soybeans and the detection method can quickly detect 12 kinds of imported transgenic soybeans approved by Ministry of Agriculture of China, including MON87701, GTS40-3-2, MON89788, CV127, A2704, A5547, DP356043, DP305423, MON87769, MON87708, 305423 is multiplied by 40-3-2, MON87701 is multiplied by MON89788;the multiple PCR kit is based on a multiple PCR method mediated by consensus primer, comprising a common primer, a multiple primer, and a multiple PCR system detection; a double annealing temperature method improves specificity and sensitivity of a multiple PCR system. The multiple PCR method is inclusive, capable of increasing detection targets, and helpful to improve detection flux of multiple PCR. The multiple PCR kit for detecting transgenic soybeans and the detection method has the advantages of being high in sensitivity, economical, fast, simple in operation and the like, and is capable of being applied to food detection.

Description

technical field [0001] The invention belongs to the field of food / gene detection, and in particular relates to a multiple PCR kit and a detection method for transgenic soybean detection. Background technique [0002] At present, the detection technologies of genetically modified crops reported at home and abroad are mainly divided into three categories, one is nucleic acid-based detection technology; the other is protein-based enzymatic and immunological detection technology; Biosensors and biochips for technological development, near-infrared spectroscopy and mass spectrometry analysis techniques based on modern analytical instruments, etc. The second type of protein-based detection technology is to detect proteins expressed by exogenous genes in transgenic plants. These proteins are easily damaged and inactivated, decomposed or disappeared during processing. The results are prone to false negatives and the method has poor repeatability. New technologies for genetically mo...

AI Technical Summary

Problems solved by technology

The second type of protein-based detection technology is to detect proteins expressed by exogenous genes in transgenic plants. These proteins are easily damaged and inactivated, decomposed or disappear during processing, and the inspection results are prone to false negatives and the repeatability of the method is poor.

New technologies for genetically modified detection developed in recent years, such as biosensors, chromatography, and near-infrared spectroscopy, are still in the research stage, and there are some shortcomings that cannot be effectively solved for the time being or exist objectively. For example, chromatography is only applicable to genetically modified Qualitative detection and analysis when major changes occur in the composition of plants and their products, this type of detection technology cannot be effectively promoted and applied in the short term

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