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Structured light illumination microscopic imaging system

A structured light illumination and microscopic imaging technology, applied in the field of optics, can solve problems such as poor stability, high cost, and difficult assembly and adjustment, and achieve high stability, low cost, and simple structure

Pending Publication Date: 2017-07-28
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Claims
  • Application Information

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Problems solved by technology

[0006] An object of the present invention is to provide a structured illumination micro-imaging system, which can solve the problems of high cost, difficult installation and adjustment, and poor stability of the existing structured illumination micro-imaging system

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Embodiment Construction

[0043] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0044] Such as figure 1 and 2 As shown, the present invention provides a structured illumination microscope imaging system, comprising:

[0045] Structured light source 1;

[0046] A beam shaping lens 2, an excitation filter 3 and a dichroic mirror 4 arranged sequentially on the emitting light path of the structured light source 1; figure 1 and 2 As shown, the beam shaping lens 2, the excitation filter 3 and the dichroic mirror 4 can be sequentially arranged on the emitting light path of the structured light source 1 from near to far;

[0047] The objective lens 5 and the sample 6 that are sequentially arranged on the first optical path of the dichroic mirror 4; figure 1 and 2 As shown, the objective lens 5 and the sample 6 can be sequentially arranged on the first optical path of the dichroic mirror 4 from near to far from the dichroic mirror 4;

[00...

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Abstract

The objective of the invention is to provide a structured light illumination microscopic imaging system. According to the invention, a light beam shaping lens, an excitation filtering sheet and a dichroscope are successively arranged on an emission light path of a structured-light light source; an objective lens and a sample are arranged on a first optical path of the dichroscope successively; and an emission filtering sheet, a cylinder mirror and a detector are successively arranged on a second optical path of the dichroscope. Compared with a structured light illumination microscopic imaging system with a compound eye lens, under the premise of reducing installation and processing precision of the structured light illumination microscopic imaging system, super-resolution microscopic images with quite high signal to noise ratios and contrast degrees can be obtained. Compared with a structured light illumination microscopic imaging system based on a digital micro-lens array or an optical grating, the system cost is greatly reduced and system stability is quite high.

Description

technical field [0001] The invention relates to the field of optics, in particular to a structured light microscopy imaging system. Background technique [0002] Structured light microscopic imaging technology is a super-resolution microscopic imaging technology based on an optical microscope. This technology uses a specially modulated structured light field to illuminate the sample, and extracts the information of the focal plane from the modulated image data of different phases by using a phase shift algorithm. , to obtain the image data of structured illumination microscopic imaging, which can break through the limitation of ordinary optical microscopes by the optical diffraction limit and achieve higher microscopic imaging resolution. [0003] Structured illumination microscopically was first realized by adding a sinusoidal grating to the illumination light path, and the grating pattern was projected onto the sample to form structured light illumination, but in these sys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/06
CPCG02B21/06G02B21/16G02B27/0955G02B5/08G02B21/006G02B27/09
Inventor 熊大曦杨西斌王沛沛
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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