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Diabetic typing kit

A technology of diabetes and kits, which is applied in the field of diabetes typing kits, and can solve the problems of time-consuming, laborious and incomplete typing of type II diabetes

Active Publication Date: 2017-07-25
广州普麦健康咨询有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of this, a kind of diabetes typing kit provided by the present invention can effectively solve the current technical defects of time-consuming and incomplete typing of type II diabetes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Sample DNA extraction, the steps are as follows:

[0099] 1. Obtain blood samples from 300 patients who have been diagnosed with type 2 diabetes in a certain hospital, and 300 patients have no typical characteristics of type 1 diabetes and other types of diabetes. Use the blood sample DNA extraction kit (Magen HiPureTissue&Blood DNA Kit D3018-03) to extract the sample DNA, and the specific steps are operated according to the kit instructions. Sample DNA was quantified and quality controlled using Nanodrop of Thermo Company.

[0100] 2. Sample DNA PCR extension

[0101] The PCR reaction system uses Taq DNA Polymerase (Vazyme, P101), which contains 10 × Taq Buffer (Mg 2+ ), dNTP Mix (10mM). For each SNP site, construct a PCR reaction system.

[0102] Each reaction system:

[0103]

[0104] 3. PCR reaction conditions: (Use the Touch Down PCR method to ensure the specificity of the expansion, and in the next 15 cycles, use a lower annealing temperature to ensure the...

Embodiment 2

[0115] According to the gene sequencing results of 300 cases of type Ⅱ diabetes in Example 1, 300 cases of diabetes were typed. In this embodiment, two typing kits are used, one is Diabetes Typing Kit A, and the other is Diabetes Typing Kit B. The specific typing results are summarized in the table below. (The subject does not have the above gene mutations, and belongs to the group that cannot be typed temporarily. This is because the pathogenesis of type 2 diabetes is very complicated, and there are still many pathogenesis related to its type that have not been elucidated).

[0116] Table 1 Statistical table for the analysis of the results of diabetes typing

[0117]

[0118] It can be seen that the diabetes typing kit A can type more than 90% of the 300 cases of the population, the diabetes typing kit B can type more than 96% of the population, and the kit B can effectively The proportion of typing is higher than A.

Embodiment 3

[0120] The typing result of embodiment 2 is verified:

[0121] 1. Take overnight fasting for 10-12 hours before drawing blood samples from 300 patients with type II diabetes mellitus in Example 2, and take venous blood on an empty stomach the next morning. Fasting blood glucose and glycosylated hemoglobin were measured on the same day; serum was collected and stored in a -80°C refrigerator for unified detection of other indicators, including fasting proinsulin, fasting insulin, and fasting C-peptide. Conventional equipment and methods are used, and the specific detection methods are as follows:

[0122] 1. The detection of blood glucose and glycosylated hemoglobin is completed by ADVIA2400 automatic biochemical analyzer;

[0123] 2. Serum insulin and proinsulin were detected by biotin-avidin double-antibody sandwich enzyme-linked immunosorbent assay (BA-ELISA). The kit was provided by Beijing East Asia Institute of Immunology Technology. The specific operation steps were carr...

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Abstract

The invention relates to the field of disease aided diagnosis, in particular to a diabetic typing kit, and discloses application of one or multiple of HNF1B gene, PDX1 gene, PAX6 gene, MADD gene, NKX2-2 gene, INSR gene, SRR gene, ND1 gene and SRIT1 gene in human whole genome in preparing diabetic typing products. The diabetic typing kit comprises a primer pair for amplifying SNP loci of one or multiple of the HNF1B gene, the PDX1 gene, the PAX6 gene, the MADD gene, the NKX2-2 gene, the INSR gene, the SRR gene, the ND1 gene or the SRIT1 gene. By the diabetic typing kit, the technical defect that current II-type diabetic typing is time-consuming, labor-consuming and incomplete can be overcome.

Description

technical field [0001] The invention relates to auxiliary diagnosis of diseases, in particular to a diabetes typing kit. Background technique [0002] With the improvement of living conditions in recent years, the incidence of diabetes is getting higher and higher, accounting for a large proportion of the total population. According to the statistics of the International Diabetes Federation (IDF), in 2011, there were 370 million people with diabetes in the world, 80% of whom were in developing countries; it is estimated that by 2030, there will be nearly 550 million people with diabetes worldwide. As a country with a large population, the number of people suffering from diabetes in my country has tripled in the past 20 years. If this trend develops, China will likely become the country with the largest number of people with diabetes in the world. The disability and fatality rate of complications caused by diabetes will generate huge medical expenses, which not only increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 陈龙钟志然廖思茵刘蓉顾炜
Owner 广州普麦健康咨询有限公司
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