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Method for preparing microbial ecological agent for treating infertility

A micro-ecological preparation and infertility technology, applied in the field of microorganisms, can solve problems such as the influence of sperm cell motility, and achieve the effects of good promotion prospects, low recurrence probability and significant treatment effect.

Inactive Publication Date: 2017-07-25
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a preparation method of probiotics for the treatment of infertility, so as to solve the technical problem that the motility of sperm cells is affected in the conventional vaginal environment

Method used

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  • Method for preparing microbial ecological agent for treating infertility

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Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method for probiotics for infertility treatment, comprising the following steps:

[0033] A. Culture and isolation of bacterial flora

[0034] (1) Preparation of medium: LB basal medium, MRS solid medium, BHI solid medium, BHI+5% skim milk medium, BSM solid medium;

[0035] (2) Take out the vaginal secretion sample preserved with glycerin from the -80 degree refrigerator, and after it melts, take an appropriate amount into a sterile 1.5ml EP tube, and use sterile PBS according to 10 1 —10 6 Doubling dilution, MRS, LB, BSM by 10 2 、10 4 Dilute BHI, BHI+5% skim milk by 10 3 、10 5 50 μL of the diluted solution was spread on solid medium, and cultured for 48 hours under anaerobic and aerobic conditions.

[0036] B. Identification of strains:

[0037] (1) In a sterile ultra-clean workbench, pick out a single colony and inoculate it in the corresponding liquid medium according to the shape, size, color, neatness of the edge, and whether the bulge is on th...

Embodiment 2

[0054] A preparation method for probiotics for infertility treatment, comprising the following steps:

[0055] 1) Isolate microorganisms from vaginal secretions, and cultivate several single colonies;

[0056] 2) carry out bacterial species identification to the microorganisms in the several single colonies, and obtain several bacterial strains;

[0057] 3) taking the several bacterial strains, co-incubating them with sperm cells in a liquid environment respectively, and screening out target bacterial strains based on the adhesion ability of each bacterial strain to sperm cells under microscope observation;

[0058] 4) Cultivate the target bacterial strain to prepare freeze-dried powder bacterial agent.

[0059] On the basis of the above technical solutions, the following conditions are met:

[0060] The medium used for isolating microorganisms in step 1) includes LB basal medium, MRS solid medium, BHI solid medium, BHI medium containing 5% (g / g) skimmed milk, and BSM solid ...

Embodiment 3

[0073] A preparation method for probiotics for infertility treatment, comprising the following steps:

[0074] 1) Isolate microorganisms from vaginal secretions, and cultivate several single colonies;

[0075] 2) carry out bacterial species identification to the microorganisms in the several single colonies, and obtain several bacterial strains;

[0076] 3) taking the several bacterial strains, co-incubating them with sperm cells in a liquid environment respectively, and screening out target bacterial strains based on the adhesion ability of each bacterial strain to sperm cells under microscope observation;

[0077] 4) Cultivate the target bacterial strain to prepare freeze-dried powder bacterial agent.

[0078] On the basis of the above technical solutions, the following conditions are met:

[0079] The medium used for isolating microorganisms in step 1) includes LB basal medium, MRS solid medium, BHI solid medium, BHI medium containing 5% (g / g) skimmed milk, and BSM solid ...

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Abstract

The invention studies the physiological action between vagina microorganisms and sperm cells with experimental means, and finds that certain vagina microorganisms can be specifically combined with sperms, so as to make sperms swim slower or even stop moving, or cause change in protein generation level of sperms to further affect the ability of sperms to be combined with eggs. Based on the findings, the invention determines a technical route for improving sperm motility by interfering with vaginal flora; microorganisms which can be well combined with sperms and do not affect basic physiological characters of sperms are selected out, adhesion of microorganisms harmful to sperms is prevented by means of the occupying function of the selected microorganisms, and therefore sperm motility can be improved, so as to relieve or treat infertility. Furthermore, probiotics microorganisms in a microbial agent are helpful for treatment of various diseases caused by vaginal flora dysbacteriosis, the treatment effect is remarkable, relapse probability is low, and popularization prospects are broad.

Description

technical field [0001] The invention relates to the technical field of microorganisms, further relates to the research on the physiological interaction between vaginal microorganisms and sperm cells, and in particular to a method for preparing a microecological preparation for infertility treatment. Background technique [0002] Female reproductive tract infection caused by vaginal microecological imbalance is a common disease in gynecology. According to statistics, infectious diseases account for more than half of the outpatients. The top three are vaginitis, cervicitis and pelvic inflammatory disease. The number of cases of bacterial vaginosis alone is millions every year, and severe cases can cause chronic cervicitis. , cervical cancer, ovarian, fallopian tube tumors and other diseases are also the main causes of infertility, premature birth, premature rupture of membranes, low birth weight premature birth, recurrent miscarriage, chorioamnionitis, etc., seriously affectin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P15/08A61P15/00C12N1/20C12R1/225C12R1/23
CPCA61K35/747C12N1/20
Inventor 陈廷涛辛洪波孟凡景
Owner NANCHANG UNIV
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