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Preparation method of Fe3O4@PEG@SiO2 artificial antibody for detecting thifensulfuron methyl

An artificial antibody, thifensulfuron-methyl technology, applied in the field of materials science, can solve the problems of high reaction temperature and non-selectivity

Active Publication Date: 2017-07-21
HEFEI UNIV
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Problems solved by technology

Although this method finally successfully produced magnetic nanospheres of 150-400nm, the reaction temperature is high (100-240°C), and it is not selective.
However, there is no preparation of Fe 3 o 4 @PEG @SiO 2 Artificial antibodies to detect thifensulfuron-methyl related literature and patent reports

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  • Preparation method of Fe3O4@PEG@SiO2 artificial antibody for detecting thifensulfuron methyl
  • Preparation method of Fe3O4@PEG@SiO2 artificial antibody for detecting thifensulfuron methyl
  • Preparation method of Fe3O4@PEG@SiO2 artificial antibody for detecting thifensulfuron methyl

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preparation example Construction

[0033] A kind of Fe that detects thifensulfuron-methyl 3 o 4 @PEG @SiO 2 The preparation method of artificial antibody, comprises in Fc 3 o 4 Surface modification of magnetic nanoparticles Polyethylene glycol 2000 (Polyethylene glycol 2000, PEG 2000), whose surface is coated with nano-SiO 2 Shell layer, forming a core-shell-shell structure, characterized in that: the elution in the artificial antibody is located at the SiO 2 Imprinted molecules in the shell, SiO 2 The interior of the shell forms a cavity structure complementary to the structure, size and functional groups of the imprinted molecule, and the artificial antibody that elutes the imprinted molecule has a specific recognition site for the target analyte molecule, achieving selective recognition of the target analyte molecule and detection, the artificial antibody preparation process includes the following three steps:

[0034] 1.1 The first step is the surface modification of polyethylene glycol 2000 water-sol...

Embodiment

[0037] Example: Using molecular imprinting technology and nano-synthesis technology to prepare Fe for detection of thifensulfuron-methyl 3 o 4 @PEG @SiO 2 Artificial antibodies, using three-step reaction to obtain the above-mentioned artificial antibodies:

[0038] 1.1 The first step is the surface modification of polyethylene glycol 2000 water-soluble magnetic nanoparticles Fe 3 o 4 Preparation: Accurately weigh 2.7030 g FeCl with an electronic balance with an accuracy of 1 / 10,000 3 ·6H 2 O and 1.9880 g FeCl 2 4H 2 O was placed in a 250 mL three-neck flask, and under a nitrogen atmosphere, 50 mL of -1 Add polyethylene glycol water 2000 solution into the above flask, heat it in a water bath to 50°C, add ammonia water at 1000 rpm, adjust the pH value of the solution to 11, react for 35 min, and magnetize Fe 3 o 4 The nanoparticles were separated from the solvent, and the Fe was washed by centrifugation with deionized water.3 o 4 Nanoparticles, Washed Fe 3 o 4 Nanopa...

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Abstract

A preparation method of Fe3O4@PEG@SiO2 artificial antibody for detecting thifensulfuron methyl comprises modifying the surface of Fe3O4 magnetic nanoparticles with polyethylene glycol 2000, coating the surface with SiO2 shell layer to form a core-shell-shell structure, diluting marker molecules in the SiO2 shell layer to form specific recognition site holes complementary with marker molecular structure, size and functionality so as to arrive at molecular selective recognition and detection for target analyses. The preparation method of the artificial antibody comprises the steps of first, preparing Fe3O4 magnetic nanoparticles, and modifying their surface with polyethylene glycol; second, adding the target molecule thifensulfuron methyl, a crosslinking agent and a catalyst, and hydrolyzing to obtain Fe3O4@PEG@SiO2 particles with surface-marked thifensulfuron methyl; third, diluting template molecule with a mixed solution of acetic acid and acetone having a volume ratio of 1:4 to obtain the Fe3O4@PEG@SiO2 artificial antibody with selective recognition marker molecules, the antibody having maximum saturated binding capacity of 41.28 mg / g for thifensulfuron methyl, the absorption rate reaches 0.45 mg / g.min within first 30 min which is 5.34 times and 3.46 times that of a non-marking method.

Description

technical field [0001] The invention relates to the field of material science, in particular to the preparation of an artificial antibody and its surface imprint detection method for thifensulfuron-methyl. Background technique [0002] Thifensulfuron methyl (thifensulfuron methyl) is a sulfonylurea herbicide, which is widely used in field weed removal because of its high herbicidal performance. However, its residues in agricultural products and the environment will bring harm to human health and cause environmental pollution. Therefore, how to quickly, accurately and selectively detect thifensulfuron-methyl residues in agricultural products and the environment is a challenging problem and an urgent problem to be solved at present. [0003] Molecular imprinting technique (MIT) is a method that uses molecular imprinting polymers (molecular imprinting polymers, MIPs) to simulate the interaction between enzyme-substrate or antibody-antigen. Molecular (template molecular) speci...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N21/33B01J20/26B01J20/30B01J20/28
CPCB01J20/06B01J20/103B01J20/268B01J20/28009G01N21/33G01N33/531
Inventor 漆天瑶高大明陈红汪丛孙虹张慧朱德春席小倩周杨群张凌云刘安求王晓晨
Owner HEFEI UNIV
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