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Tissue culture and rapid propagation method of traditional Chinese medicine sinomenium acutum

A tissue culture fast propagation and ivy technology, applied in the field of plant tissue culture, can solve the problems of no ivy tissue culture technology, no patent application for ivy tissue culture fast propagation, etc., to promote the development of plant resources and shorten the cycle. , the effect of low cost

Inactive Publication Date: 2017-07-21
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no report on the tissue culture technology of ivy at home and abroad, and there is no application for the patent of ivy tissue culture and rapid propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Collection of explants: In the wild, the middle and upper parts of ivy plants that grow vigorously and have no obvious diseases, and the canes with joints that are sunny and full are selected as explants. After collection, they are immediately treated with water retention and moisturizing and brought back to the laboratory in time.

[0020] (2) Explant induction: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 10 seconds, rinsed with sterile water for 3 After the first time, dry the water with sterile filter paper, sterilize it in 0.1% mercuric chloride solution for 20 minutes, wash it with sterile water for 5 times, dry it with sterile filter paper, and cut it into 2.0-2.5cm vines with knots. The stems are inoculated into the induction medium, placed at 25° C. for 40 days in full dark culture to induce the formation of adventitiou...

Embodiment 2

[0026] (1) Collection of explants: In the wild, the middle and upper parts of ivy plants that grow vigorously and have no obvious diseases, and the canes with joints that are sunny and full are selected as explants. After collection, they are immediately treated with water retention and moisturizing and brought back to the laboratory in time.

[0027] (2) Explant induction: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 13 seconds, rinsed with sterile water for 4 After the first time, dry the water with sterile filter paper, sterilize it in 0.1% mercuric chloride solution for 25 minutes, rinse it with sterile water for 6 times, dry it with sterile filter paper, and cut it into 2.0-2.5cm vines with knots. The stems are inoculated into the induction medium, placed at 27° C. for 48 days in full dark culture to induce the formation of adventitio...

Embodiment 3

[0033] (1) Collection of explants: In the wild, the middle and upper parts of ivy plants that grow vigorously and have no obvious diseases, and the canes with joints that are sunny and full are selected as explants. After collection, they are immediately treated with water retention and moisturizing and brought back to the laboratory in time.

[0034](2) Explant induction: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 15 seconds, rinsed with sterile water for 5 seconds After the first time, dry the water with sterile filter paper, sterilize it in 0.1% mercuric chloride solution for 30 minutes, wash it with sterile water for 7 times, dry it with sterile filter paper, and cut it into 2.0-2.5cm vines with knots. The stems are inoculated into the induction medium, placed at 28° C. for 60 days in full dark culture to induce the formation of adve...

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PUM

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Abstract

The invention relates to a method of plant tissue culture in an agricultural biotechnology, in particular to a tissue culture and rapid propagation method of a traditional Chinese medicine sinomenium acutum. The method sequentially comprises the following steps of obtaining an explant of the sinomenium acutum, performing explant induction culture, performing enrichment culture, performing rooting culture, and then performing transplanting, wherein an explant induction culture medium for explant induction culture operation comprises a B5 culture medium, 1.0-3.0mg / L of 6-BA, 0.5-1.0mg / L of NAA, 0.05-0.1mg / L of GA3, 25-30g / L of sucrose, 3.5-4.0g / L of agar, and 0.5-1.0g / L of active carbon; and pH (potential of hydrogen) of the explant induction culture medium is 5.4-5.8. According to the method, a jointed stem section of the sinomenium acutum is selected as the explant, and the steps of induction, enrichment, rooting, seedling exercising and transplanting are performed, so that an induction rate of the sinomenium acutum reaches above 89%; a survival rate of the sinomenium acutum reaches above 90%; tissue culture and rapid propagation of the traditional Chinese medicine sinomenium acutum are realized; and a purpose of the invention is realized.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Chinese medicine ivy. Background technique [0002] Sinomenium acutum, also known as Sinomenium acutum, also known as Sinomenium acutum, Xunfengteng, Fufengteng, Fengfengteng, etc., is the dry rhizome of plants of the family Fangcai, which is rich in sinomenine and bisinomenine , Tetrahydroepipodine, Tetradine, Teramenine, Magnoline, Isosinine, Tetradrine, Pyrenine, etc., have the effects of dredging collaterals, relieving pain, and expelling rheumatism, and are often used for rheumatism and rheumatoid Treatment of arthritis, joint swelling, limb pain, numbness and other diseases. As a traditional Chinese medicine with unique medicinal effects, it has been used for more than one thousand years in folks to treat rheumatic diseases by oral administration or external application of ivy or its comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁钧淞莫昭展杨业容韦敏
Owner YULIN NORMAL UNIVERSITY
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