Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tissue-culture and rapid-propagation method for traditional Chinese medicine capparis versicolor

A technique for tissue culture and rapid propagation of chickens, which is applied in the field of tissue culture and rapid propagation of traditional Chinese medicine chickens, can solve problems such as the absence of chickens and the application of patents for tissue culture and rapid propagation of chickens, and achieves Low cost, promotion of plant resources development, high reproduction coefficient effect

Inactive Publication Date: 2019-08-09
YULIN NORMAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no report on the tissue culture technology of Qutou chicken at home and abroad, and there is no application for a patent for tissue culture and rapid propagation of Qutou chicken

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Collection of explants: In Bobai County, Guangxi, the middle and upper segmented stems of the vigorously growing Qutou chicken plants without obvious diseases are selected as explants, and the explants are immediately treated with water retention and moisturizing and brought back in time laboratory.

[0020] (2) Explant induction: The explants collected in step (1) and returned to the laboratory were rinsed under tap water for 4 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 20 seconds, sterile water Rinse 5 times, blot dry with sterile filter paper, sterilize in 0.1% mercuric chloride solution for 20 minutes, rinse 8 times with sterile water, blot dry with sterile filter paper, cut into 2.0-2.5cm strips The nodal stem segments are inoculated into the induction medium, placed at 25° C. for 30 days in full dark culture to induce the formation of adventitious buds, the induction rate reaches 95%, and the pollution rate is lower th...

Embodiment 2

[0026] (1) Collection of explants: The explants were selected from Luchuan County, Guangxi Province, which grew vigorously and vigorously with no obvious diseases, and the mid-upper section of the stem with joints in the sun. After collection, it was immediately treated with water retention and moisturizing and brought back to the laboratory in time. .

[0027] (2) Explant induction: the explants collected in step (1) and returned to the laboratory were rinsed under tap water for 4 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 18 seconds, sterile water Rinse 5 times, blot dry with sterile filter paper, sterilize in 0.1% mercuric chloride solution for 18 minutes, rinse 7 times with sterile water, blot dry with sterile filter paper, cut into 2.0-2.5cm strips The nodal stem segments are inoculated into the induction medium, placed at 27° C. for 32 days in full dark culture to induce the formation of adventitious buds, the induction rate reac...

Embodiment 3

[0033] (1) Collection of explants: Yangjiang County, Guangdong Province selects the middle and upper part of the stem with no obvious disease and is full of sunny and full stems of the vigorously growing Qutou chicken plant as the explants. Immediately after collection, water retention and moisturizing treatment are carried out and brought back to the laboratory in time. .

[0034](2) Explant induction: The explants collected in step (1) and returned to the laboratory were rinsed under tap water for 4 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 15 seconds, sterile water Rinse 5 times, blot dry with sterile filter paper, sterilize in 0.1% mercuric chloride solution for 15 minutes, rinse 5 times with sterile water, blot dry with sterile filter paper, cut into 2.0-2.5cm strips The nodal stem segments are inoculated into the induction medium, placed at 28° C. for 35 days in full dark culture to induce the formation of adventitious buds, the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a plant tissue culture method in an agricultural biotechnology, in particular to a tissue-culture and rapid-propagation method for traditional Chinese medicine capparis versicolor. The method comprises the following successive steps of acquisition of an explant of the capparis versicolor; induction culture of the explant; enrichment culture; rooting culture; transplantation. An explant induction culture medium through induction culture operation of the explant comprises a Nitsch culture medium, 0.5-1.5 mg / L of KT, 0.2-0.4 mg / L of IBA, 0.01-0.03 mg / L of chlormequat chloride, 25-30 g / L of saccharose, 3.0-4.0 g / L of agar and 0.5-1.0 g / L of activated carbon, wherein the pH is 5.5-5.8. According to method, by selecting stems, with segments, of the capparis versicolor asthe explant, through the steps such as induction culture, enrichment culture, rooting culture and transplantation, the induction rate of the capparis versicolor reaches 90% or above, the survival rate reaches 95% or above, tissue culture and rapid propagation of the capparis versicolor are achieved, and accordingly the aim is achieved.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of traditional Chinese medicine crocodile chicken. Background technique [0002] Capparis versicolor Griff (Capparis versicolor Griff) is a plant of the genus Capparis in the family Capparis. Produced in Guangdong and Guangxi, it likes to be born in sparse forests or shrubs on slightly dry sandy soil at an altitude of 200-2000m. The leaves are alternate and puberulent when young; the leaves are papery, oval or oblong-elliptic, with apex Blunt or acute and blunt head, base broadly cuneate and slightly rounded, margin entire. Umbels axillary or terminal, with 2-4 flowers; sepals 4, outer 2 round ovate, inner 2 elliptic, petals white or light red, obovate, longer than sepals; fruit spherical, 3-5cm in diameter, Black when mature, surface rough, sometimes with a few nodular or verrucous protrusions; ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 莫昭展林雨
Owner YULIN NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com