Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method of tissue culture and rapid propagation of Guangxi characteristic Yao medicine Sifangteng

A technology for tissue culture rapid propagation and Sifang vine, which is applied in the field of plant tissue culture, can solve the problems that there is no patent application for Sifang vine tissue culture and fast propagation, and no Sifang vine tissue culture technology, etc., to promote the development of plant resources and shorten the cycle. , the effect of high reproduction coefficient

Inactive Publication Date: 2018-11-27
YULIN NORMAL UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no report on the tissue culture technology of Sifangteng at home and abroad, and there is no application for the patent of Sifangteng tissue culture and rapid propagation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Collection of explants: In the field, the middle and upper part of the Sifang rattan plant with no obvious diseases and the sunny and substantial cane with joints were selected as explants. After collection, water retention and moisturizing treatment was carried out immediately and brought back to the laboratory in time.

[0020] (2) Explant induction: The explants collected in step (1) and returned to the laboratory were rinsed under tap water for 3 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 10 seconds, sterile water After rinsing 3 times, dry the water with sterile filter paper, sterilize in 0.1% mercuric chloride solution for 15 minutes, rinse with sterile water 5 times, dry the water with sterile filter paper, cut into strips of about 2.0-2.5 cm Canes were inoculated into the induction medium, placed at 25°C for 25 days in total darkness to induce the formation of adventitious buds, the induction rate reached 94.1%, and t...

Embodiment 2

[0026] (1) Collection of explants: In the field, the middle and upper part of the Sifang rattan plant with no obvious diseases and the sunny and substantial cane with joints were selected as explants. After collection, water retention and moisturizing treatment was carried out immediately and brought back to the laboratory in time.

[0027] (2) Explant induction: The explants collected in step (1) and returned to the laboratory were rinsed under tap water for 4 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 13 seconds, sterile water Rinse 4 times, blot dry with sterile filter paper, sterilize in 0.1% mercuric chloride solution for 17 minutes, rinse 6 times with sterile water, blot dry with sterile filter paper, cut into 2.0-2.5cm strips The canes are inoculated into the induction medium, placed at 26°C for 31 days in full dark culture to induce the formation of adventitious buds, the induction rate reaches more than 90%, and the pollution r...

Embodiment 3

[0033](1) Collection of explants: In the field, the middle and upper part of the Sifang rattan plant with no obvious diseases and the sunny and substantial cane with joints were selected as explants. After collection, water retention and moisturizing treatment was carried out immediately and brought back to the laboratory in time.

[0034] (2) Explant induction: The explants collected in step (1) and returned to the laboratory were rinsed under tap water for 5 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 15 seconds, sterile water Rinse 5 times, blot dry with sterile filter paper, sterilize in 0.1% mercuric chloride solution for 20 minutes, rinse 7 times with sterile water, blot dry with sterile filter paper, cut into 2.0-2.5cm strips Canes were inoculated into the induction medium, placed at 28°C for 35 days in total darkness to induce the formation of adventitious buds, the induction reached 88.5%, and the pollution rate was lower than 8...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a tissue culture rapid propagation method for cissus pteroclada hayata serving as characteristic herbal medicine of Guangxi Yao nationality. The tissue culture rapid propagation method sequentially comprises the steps of collection of an explant of a cissus pteroclada hayata plant, explant induction culture, multiplication culture, rooting culture and transplanting, wherein an explant induction medium used in the explant induction culture operation is prepared from an MS culture medium, 0.5 to 2.0mg / L 6-BA, 0.2 to 0.5mg / L NAA (Naphthalene Acetic Acid), 0.01 to 0.05mg / L Paclobutrazol, 25 to 30g / L sucrose, 3.5 to 4.0g / L agar and 0.5 to 1.0g / L active carbon, and is 5.4 to 5.8 in pH. A cissus pteroclada hayata stem segment with node is selected as the explant, and is subjected to the steps of induction culture, multiplication culture, rooting culture and transplantation, the induction rate is 88.5 percent or more, and the survival rate is 95 percent or more, so that tissue culture rapid propagation of the cissus pteroclada hayata is realized, thereby fulfilling the aim of the invention.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Sifangteng, a characteristic Yao medicine in Guangxi. Background technique [0002] Sifang vine (Cissus Pteroclada Hayata), also known as red Sifang vine, winged vine, spring root vine, etc., is a herbaceous vine of the genus Cissus Linn. of the family Vitaceae. It often grows in broad-leaved forests and is a characteristic of Guangxi. One of the medicines of the Yao nationality, mainly distributed in Baise, Luchuan, Hezhou, Bobai, Jingxi and other places. It is widely used among the people to treat rheumatic arthralgia, lumbar muscle strain, trauma, and hemostasis, etc., with remarkable curative effect. According to the records of "Guangxi Traditional Chinese Medicine", the Sifang cane can be used as medicine, and its taste is slightly bitter, astringent, sweet, and flat in nature. It has the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁钧淞
Owner YULIN NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com