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Method for generating hybrid liriodendron somatic embryo by using jasmonic acid methyl ester

A technology of methyl jasmonate and hybrid Liriodendron, which is applied in the field of tissue culture seedlings, can solve the problems of reducing the induction rate of somatic embryos and reducing the number of somatic embryos, so as to improve the generation efficiency of somatic embryos, reduce the rate of deformities, and improve the quality of somatic embryos. Effect on Maturity Rate

Active Publication Date: 2017-07-21
NANJING FORESTRY UNIV
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Problems solved by technology

Chen Xiaopeng found that high temperature can reduce the number of somatic embryos and reduce the induction rate of somatic embryos in the research of cucumber somatic embryogenesis

Method used

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  • Method for generating hybrid liriodendron somatic embryo by using jasmonic acid methyl ester
  • Method for generating hybrid liriodendron somatic embryo by using jasmonic acid methyl ester
  • Method for generating hybrid liriodendron somatic embryo by using jasmonic acid methyl ester

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Experimental program
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Embodiment 1

[0050] Induction of the embryogenic callus of the immature embryo of embodiment 1

[0051] 1. Seed sterilization

[0052] Before sterilization, the aggregated samara should be cut open to remove the seed wings, and the seeds should be washed with water for 30 minutes. Then carry out sterilization treatment in the ultra-clean workbench, put the seeds in a sterile triangular flask, add 75% ethanol, sterilize for 30 seconds, pour out the 75% ethanol and add 0.1% mercuric chloride, sterilize for 8-10 minutes, pour Remove the mercuric chloride solution, wash with sterile water 3 to 4 times, seal and store at 4°C after the treatment, and proceed to the next step of treatment.

[0053] 2. Induction of embryogenic callus

[0054] The seed coat was removed in a sterile environment, and immature embryos and endosperms were placed in 3 / 4MS medium supplemented with 2,4-D, BA and other hormones for callus induction culture. The culture conditions were 23°C, dark culture, The subculture ...

Embodiment 2

[0094] After a certain number of subcultures, the state of the callus was yellow and fine granule, the texture was firm, and the growth rate was relatively slow. At this time, the embryogenic callus of Liriodendron chinensis was in a better state. When the embryogenic callus grows and is in good condition, somatic embryo induction is carried out.

[0095] Directly use solid medium for somatic embryo induction, and put the callus in a good state directly on the somatic embryo induction medium for culture. Hybrid Liriodendron somatic embryo induction medium: 3 / 4MS+VC 5mg / L+ CH0.2g / L+activated carbon 2g / L+sucrose 40g / L+agar powder 8g / L, add MeJa and ABA (as shown in Table 3) of different concentrations in the culture medium, with not adding MeJa and ABA and only adding 2.0mg / L The medium of LABA was used as the control, and the effects of different concentrations of methyl jasmonate in the solid medium on the somatic embryogenesis of Liriodendron chinensis were observed.

[0096...

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Abstract

The invention discloses a method for generating a hybrid liriodendron somatic embryo by using jasmonic acid methyl ester. The method comprises the following steps: seed sterilization, induction of embryonic callus, establishment of an embryogenic cell suspension line, acquisition and transition of liquid single cells, and induction of generation of the hybrid liriodendron somatic embryo. The method is characterized in that when generation of the hybrid liriodendron somatic embryo is induced, the jasmonic acid methyl ester and ABA are added in the culture medium, or the jasmonic acid methyl ester is independently added in the culture medium. According to the method for generating the hybrid liriodendron somatic embryo by using the jasmonic acid methyl ester, a plant growth regulator MeJa is added, the inductivity of the somatic embryo is increased effectively, the abnormal rate is reduced, the generating efficiency of the hybrid liriodendron somatic embryo is improved further, the practicability is good, and establishment of a hybrid liriodendron rapid propagation system is facilitated.

Description

technical field [0001] The invention belongs to the technical field of tissue culture seedlings, and in particular relates to a method for utilizing methyl jasmonate (MeJa) to perform somatic embryogenesis of a hybrid tulip tree. Background technique [0002] In recent years, people have continuously increased the research on plant somatic embryogenesis, which has achieved rapid development and achieved great results in decades. Because the somatic embryos induced by somatic cells have the characteristics of large number, fast growth, complete structure, and high reproductive efficiency, somatic embryogenesis technology has become an effective means for rapid plant reproduction, which is conducive to the preservation of excellent forest species and shortens the time for cultivating excellent varieties. Time, speeding up the development of industrialization, etc. are of great significance in the development of forestry. [0003] Plant somatic embryogenesis is an effective wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 陈金慧孟岩施季森成铁龙杨立明张稼霁陆叶
Owner NANJING FORESTRY UNIV
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