Preparation method of human body lubricant on the basis of probiotic culture substance
A human body lubricant and probiotic technology, applied in the field of microorganisms, can solve the problems of human health hazards and the inability to protect the micro-ecosystem of the female vagina, and achieve good therapeutic effects, reduce the risk of colonization, and avoid bacterial infections.
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Embodiment 1
[0023] A method for preparing a human body lubricant based on a probiotic culture, comprising the following steps:
[0024] A. Cultivation of probiotics: select probiotics after secondary activation and inoculate them into the culture medium for 24-48 hours.
[0025] The probiotics are: one or more composites of Lactobacillus crispatus, Lactobacillus jansneri, Lactobacillus gasseri, Lactobacillus vaginal, Lactobacillus johnsonii, Lactobacillus acidophilus or Lactobacillus fermentum;
[0026] B. Freeze-drying of probiotics: the cultured probiotics are collected by centrifugation, and placed in a freeze dryer for overnight freeze-drying; the collected freeze-dried powder is placed in a -70 degree refrigerator for later use;
[0027] C, the preparation of microcapsules: choose sodium alginate as capsule material, take pectin as auxiliary material, the concentration of pectin solution is 2%, the volume ratio of pectin solution and probiotic solution is 1:1, sodium alginate and pec...
Embodiment 2
[0033] A method for preparing a human body lubricant based on a probiotic culture, comprising the following steps:
[0034] 1) Inoculate the probiotic bacterial strains in the culture medium, cultivate for 24 hours, collect the bacterial cells and freeze-dry to obtain bacterial powder;
[0035] 2) taking the probiotics and culturing them in a liquid medium to obtain a probiotic culture solution, preparing an aqueous pectin solution with a mass concentration of 2%, mixing the probiotic culture solution with an equal volume of the pectin solution with a mass concentration of 2%, Add sodium alginate twice the mass of pectin to the mixture, then add CaCl 2 To a final concentration of 0.3mol / L, mix well to obtain probiotic microcapsules;
[0036] 3) Take a commercially available human body lubricant, add the bacteria powder and probiotic microcapsules into it, and mix well.
[0037] On the basis of the above technical solutions, the following conditions are met:
[0038] The pro...
Embodiment 3
[0045] A method for preparing a human body lubricant based on a probiotic culture, comprising the following steps:
[0046] 1) Inoculate the probiotic bacterial strains in the culture medium, cultivate for 48 hours, collect the bacterial cells and freeze-dry to obtain bacterial powder;
[0047] 2) taking the probiotics and culturing them in a liquid medium to obtain a probiotic culture solution, preparing an aqueous pectin solution with a mass concentration of 2%, mixing the probiotic culture solution with an equal volume of the pectin solution with a mass concentration of 2%, Add sodium alginate twice the mass of pectin to the mixture, then add CaCl 2 To a final concentration of 0.3mol / L, mix well to obtain probiotic microcapsules;
[0048] 3) Take a commercially available human body lubricant, add the bacteria powder and probiotic microcapsules into it, and mix well.
[0049] On the basis of the above technical solutions, the following conditions are met:
[0050] The pro...
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