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Preparation method of mussel polypeptide oral liquid

A technology of peptide oral liquid and mussel, which is applied in the field of preparation of mussel polypeptide oral liquid, can solve the problems of mussel not getting involved in the beverage industry and the instability of mussel enzymatic hydrolyzate, so as to improve emulsification stability and reduce water solubility. Decrease in characteristics and reduce the effect of particle size increase

Inactive Publication Date: 2017-07-07
DALIAN POLYTECHNIC UNIVERSITY
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  • Description
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AI Technical Summary

Problems solved by technology

With the in-depth research on mussel processing technology, more enzymatically active peptides have been extracted, but due to the instability of mussel enzymatic hydrolyzate, mussels have not been involved in the beverage industry, and mussel peptide oral liquid has also No reported studies

Method used

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Experimental program
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Embodiment Construction

[0016] The invention will be further explained below in conjunction with specific embodiments.

[0017] (1) Blue mussel sample pretreatment: Wash the purchased fresh blue mussel thoroughly with tap water, and then rinse with deionized water; remove the shell and shreds; chop the mussel meat; 5000-10000rpm / 2 -5min homogenate;

[0018] (2) Enzymatic hydrolysis: the ratio of solid to liquid is 1:2-1:5; adjust the pH of the solution with 1mol / L HCl and 0.5mol / L NaOH respectively; choose 3000-5000U / g of pepsin, trypsin and neutral protease in Enzymolysis at pH2, pH8.5, pH7.5, 37°C, 45°C, 40°C for 60-180min; add dropwise 0.1mol / L HCl, 0.2mol / L NaOH to keep the pH of the solution constant during the enzymolysis process; After the enzymatic hydrolysis is completed, the enzyme is inactivated in a boiling water bath for 10 minutes; the enzymatic hydrolysis solution is centrifuged at 8000-10000 rpm for 10-20 minutes to obtain the mussel polypeptide enzymatic hydrolysis solution.

[001...

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Abstract

The present invention aims at providing a preparation method of novel mussel polypeptide oral liquid by using an enzymolysis technology of mainly using trypsin, pepsin and neutral protease to conduct enzyme digestion of purple mussels, and adding an emulsifier, a stabilizer and a seasoning material, and provides the new mussel polypeptide oral liquid product for the industry of functional food. The enzymolysis technology of different enzymes is used and the enzymolysis is conducted under the enzymolysis conditions at different enzyme activities, temperatures, pH and time. The preparation method uses different types of the protein stabilizer, emulsifier and flavoring agent, reduces the polymerization trend between protein molecules, improves the emulsion stability of the system, and reduces the problems of enlarged particles and decreased water soluble properties caused by fat precipitation.

Description

technical field [0001] The invention relates to a preparation method of mussel polypeptide oral liquid. Background technique [0002] my country is currently the country with the largest mussel production in the world, with an annual output of more than 1 million tons. Due to backward processing technology, mussels in my country are mainly used for export of fresh and frozen products, resulting in low added value of mussels and serious waste of protein resources. Moreover, as a nourishing dietary supplement, blue mussels have a certain effect on symptoms such as liver and kidney deficiency, dizziness and night sweats, frail anemia, low back pain, senile hypertension, and arteriosclerosis, and many studies have also shown that mussel peptides have Significant anti-inflammatory, anti-microbial, anti-coagulant, anti-cancer, hypotensive and other functional activities. [0003] As an important marine economic shellfish, mussels are rich in active polysaccharides, functional po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L17/40A23L33/00
CPCA23V2002/00A23V2200/30A23V2250/1614A23V2250/5086A23V2250/5112A23V2200/06A23V2200/222
Inventor 杜明毛凤娇乔美玲王震宇涂茂林郭子璇祁立波
Owner DALIAN POLYTECHNIC UNIVERSITY
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