Preparation method of immunoaffinity column

Abstract

The invention discloses a preparation method of an immunoaffinity column. The immunoaffinity column is a compound affinity column which is mainly prepared from three types of immunoaffinity columns through mixed packing in a volume ratio being 1:1:1, and the three types of immunoaffinity columns are prepared from three antibodies and a coupling matrix through coupling and wet packing. The column capacity of the immunoaffinity column is 2,500 ng, the immunoaffinity column is good in purification effect and can be repeatedly used three times, and average recovery rate is 67%-107%. The compound immunoaffinity column has good specific adsorption property for at least 12 sulfonamides including sulfaquinoxaline, sulfadiazine, sulfapyridine, sulfametoxydiazine, sulfadimidine, sulfamethoxazole, sulfafurazole, sulfadimethoxypyrimidine, sulfathiazole, sulfamerazine, sodium N-sulphanilamidate and sulfamonomethoxine. The immunoaffinity column which is high in coupling rate, specific adsorption property, column capacity and recovery rate is prepared, and a sensitive, rapid and simple analysis method capable of detecting residues of the 12 sulfonamides simultaneously is provided.

Description

technical field [0001] The invention relates to the preparation of an immunoaffinity column for detecting and purifying sulfonamide drug residues, and belongs to the technical fields of immunology and food detection and quarantine. Background technique [0002] Sulfonamide drug residues in animal-derived foods are a class of veterinary drugs that are widely concerned. These residues will cause allergic reactions in the human body after entering the human body, and may have certain carcinogenicity. Therefore, the research on sulfa drug residues has become the focus of food inspection workers. The interference of the matrix in the detection process is relatively large. The current detection purification methods mainly include solid-phase extraction, liquid-liquid extraction, etc., the operation is relatively cumbersome, and the pretreatment of animal-derived samples is complicated; the selectivity is relatively low, and the purification effect is not ideal. There is a lot of ...

AI Technical Summary

Problems solved by technology

The interference of the matrix in the detection process is relatively large. The current detection purification methods mainly include solid-phase extraction, liquid-liquid extraction, etc., the operation is relatively cumbersome, and the pretreatment of animal-derived samples is complicated; the selectivity is relatively low, and the purification effect is not ideal. High interference; poor recovery and repeatability

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