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Chloroalkene determinating device and detection method thereof

The technology of a chlorinated olefin and a measuring device, which is applied in the field of chlorinated olefin measuring device, can solve the problems of complicated steps such as solvent extraction and purging and trapping, difficult to directly degrade by microorganisms, expensive experimental equipment, etc., and achieves high reproducibility. , The effect of shortened reaction time and low cost

Inactive Publication Date: 2017-06-30
HOHAI UNIV
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  • Description
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Problems solved by technology

Since chlorinated olefins are volatile chlorine-containing aliphatic compounds and are difficult to be directly degraded by microorganisms, there are very few studies on the determination of chlorinated olefins using biosensors
The traditional determination method is gas chromatography, which takes a long time to analyze, requires pretreatment, and the steps of solvent extraction and purge and trap are relatively complicated, and the experimental equipment is relatively expensive

Method used

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  • Chloroalkene determinating device and detection method thereof
  • Chloroalkene determinating device and detection method thereof
  • Chloroalkene determinating device and detection method thereof

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Embodiment 1

[0034] The content of trichlorethylene was determined by the degradation of trichlorethylene by methane-oxidizing bacteria OB3b, and the composition of the medium used for the growth of methane-oxidizing bacteria OB3b was: 1.0g MgSO 4 ·7H 2 O; 0.2 g CaCl 2 ; 0.004g EDTA iron salt; 1.0g KNO 3 ; 0.05% trace element solution (2.86g H 3 BO 3 , 1.81g MnCl 2 4H 2 O, 0.22g ZnSO 4 ·7H 2 O, 0.08g CuSO 4 ·5H 2 O, 0.021g Na 2 MoO 4, add sterile water to 1L, and finally add 1 drop of concentrated sulfuric acid), dissolve in 1L of distilled water, adjust the pH of the medium to about 7.0, and finally add 2 mL of sterile phosphate buffer (pH 7.0) . Under the condition of 30°C, the methanotroph OB3b was inoculated into the above-mentioned medium, and cultured in a gas environment of methane:air (1:2) at a rotation speed of 250rpm until the stationary phase.

[0035] The methanotrophs OB3b cultivated to the stationary phase were centrifuged at 4°C and 8000rpm for 5min, and washed...

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Abstract

The invention relates to a chloroalkene determinating device and a detection method thereof, and belongs to the technical field of environment monitoring. The chloroalkene determinating device comprises a water bath device, a biosensor device, a biomembrane device, an electromagnetic stirring device and a detection device, wherein the water bath device provides a constant-temperature environment; the biosensor device comprises an AgCl / Ag2S membrane working electrode and an Ag / AgCl reference electrode; the biomembrane device is fixed below the working electrode, and comprises two porous nitrocellulose membranes and degrading bacteria fixed between the two membranes; the electromagnetic stirring device ensures uniform mixing of a buffer solution; the detection device is used for determinating chloride ion concentration after the bacteria degrade chloroalkene, and finally converting the chloride ion concentration into the concentration of the determinated chloroalkene. The content of the chloroalkene is determinated through microbial degradation, so that secondary pollution is avoided; the chloroalkene determinating device is simple and reusable; the reaction time is very short; the detection limit is low; the reproducibility is high; the chloroalkene determinating device can resist interference caused by temperature and pH fluctuations within a certain range; efficient and accurate determination of the chloroalkene in groundwater is ensured.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to a chlorinated olefin measuring device and a detecting method thereof. Background technique [0002] Insufficient water sources and water source pollution are the most immediate problems facing China's drinking water sources, and the problem of drinking water source pollution is particularly prominent. At present, about 97% of China's cities have groundwater pollution, and the degree is divided into serious pollution, moderate pollution and light pollution, and the basic clean groundwater is only 3%. Groundwater pollution is a phenomenon in which the quality of groundwater decreases due to changes in the chemical composition, physical properties, and biological characteristics of groundwater caused by human activities. The main causes of groundwater pollution are: direct discharge of industrial wastewater to the ground, intrusion of polluted surface water into the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416
CPCG01N27/416
Inventor 李轶杨楠张弛都基铭王磊
Owner HOHAI UNIV
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