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AuNC (gold nanocluster) and CD (carbon quantum dot) composite fluorescent probe and application thereof

A technology of carbon quantum dots and gold nanoclusters is applied in the application field of detection, which can solve the problem that carbon quantum dots do not have quantum confinement effect, etc., and achieve the effects of no toxic side effects, good selectivity, high sensitivity and selectivity

Inactive Publication Date: 2017-06-30
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, carbon quantum dots do not have the size-dependent quantum confinement effect similar to other fluorescent carbon nanomaterials.

Method used

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  • AuNC (gold nanocluster) and CD (carbon quantum dot) composite fluorescent probe and application thereof
  • AuNC (gold nanocluster) and CD (carbon quantum dot) composite fluorescent probe and application thereof
  • AuNC (gold nanocluster) and CD (carbon quantum dot) composite fluorescent probe and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1. A method for preparing bovine serum albumin functionalized gold nanoclusters (BSA-AuNCs), which is characterized by comprising the following steps:

[0041] Under constant stirring, take 5 mL, 10 mM chloroauric acid solution and add 5 mL of 50 mg / mL BSA solution, after 2 minutes, add 0.5 mL, 1 M NaOH solution, and then mix the solution at 37 Stirring was continued for 12 hours at °C. The color of the solution changed from the bright yellow of the original chloroauric acid solution to dark brown. The color of the BSA-AuNCs solution was observed under an ultraviolet light source (254 nm), and the fluorescence spectrum changes of its absorption and emission peaks were detected with a Perkin Elmer LS-55 fluorescence spectrometer. The prepared BSA-AuNCs exhibited red light emission under the ultraviolet light source. The fluorescence spectrum was measured and excited at a wavelength of 470 nm. BSA-AuNCs had a strong emission peak at 640 nm. Adding Hg 2+ Then ...

Embodiment 2

[0042] Embodiment 2, the preparation of fluorescent carbon quantum dots:

[0043] Add 2.10 g of citric acid to 3.5 g (35 mmol) of diethylenetriamine (DETA), and heat the mixed reactant in an oil bath. The reactant is heated from room temperature to 170 oC, and heated at 170 Lower heat for 30 minutes. The reaction mixture was then removed from the oil bath and allowed to cool to room temperature. Acetone was added to the reactant, and the precipitate was collected by centrifugation. The resulting white solid was placed in a dialysis bag (3.0 kDa) and dialyzed against water for two days to remove small molecules. The water was changed every six hours, and the obtained yellow solid that was freeze-dried in the dialysis bag was carbon quantum dots. The prepared fluorescent carbon quantum dots exhibit blue light emission under the ultraviolet light source. The fluorescence spectrum is measured with a Perkin Elmer LS-55 fluorescence spectrophotometer. When excited at a wavelength...

Embodiment 3

[0044] Example 3, Preparation and detection application research of BSA-AuNCs and carbon quantum dot multifunctional composite probe:

[0045] Study the effect on the fluorescence intensity of the mixed solution by adjusting the material ratio of BSA-AuNCs and carbon quantum dots: by controlling the amount of BSA-AuNCs diluted 10 times, changing the nano The amount of carbon quantum dots diluted 150 times the solution makes the molar ratio of the two 1: 1, 1: 2, 1: 3, 1: 4, 1: 5. Each group of experiments is done in parallel for 3 times, and the obtained mixed solution is measured respectively. Fluorescence intensity, choose the molar ratio 1: 3 with the maximum fluorescence intensity as the optimal reaction condition. The prepared mixed solution of gold nanoclusters and carbon quantum dots (AuNCs-CDs) can detect trace amounts of heavy metal ions Hg contained in aqueous samples by fluorescence detection. 2+ and Cr 6+ , the size of the AuNCs-CDs multifunctional fluorescent pr...

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Abstract

The invention discloses an AuNC (gold nanocluster) and CD (carbon quantum dot) composite fluorescent probe. The AuNC and CD composite fluorescent probe comprises BSA (bovine serum albumin) functionalized AuNCs (BSA-AuNCs) and fluorescent CDs in the mole ratio being 1:(1-5), wherein the BSA-AuNCs are prepared from a chloroauric acid solution, a BSA 4 solution and an NaOH solution through a reaction, and the fluorescent CDs are prepared from citric acid, diethylenetriamine and acetone through a reaction; the BSA-AuNCs and the CDs constituting the multifunctional composite fluorescent probe have specific recognition response to Hg<2+> and Cr<6+> respectively, when the BSA-AuNCs and the CDs constitute the multifunctional composite fluorescent probe, fluorescence emission of the BSA-AuNCs and fluorescence emission of the CDs are not affected mutually, respective probe detection results are undisturbed, the probe has higher sensitivity and selectivity, and respective detection of different kinds of ions and distinctive effects when two kinds of ions exist simultaneously are realized.

Description

technical field [0001] The invention belongs to the technical field of nanometer detection of ions, in particular to gold nanoclusters and carbon quantum dot composite fluorescent probes and their ability to detect heavy metal ions Hg 2+ and Cr 6+ detection applications. Background technique [0002] Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. Among heavy metal ions, mercury is a highly toxic but ubiquitous pollutant in nature. Mercury pollution can cause serious damage to the brain, nervous system, endocrine system, and kidneys. Water-soluble mercury ions (Hg 2+ ) is one of the most common and stable forms of mercury ion pollutants, which is the main way of water environment and soil pollution. It is mainly caused by human factors such as mining, exhaust gas discharge, sewage irrigation and use of heavy metal products. Inorganic mercury ions can be converted to methylmercury by bacteria in the environment, and thi...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 柴芳于丽颖任国娟李双祝宝雅曲凤玉
Owner HARBIN NORMAL UNIVERSITY
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