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Active-probiotic-containing feed additive as well as preparation method and application thereof

A feed additive and active probiotic technology, which is applied in the field of feed research, can solve the problems of large differences in microbial fermentation production and application effects, complex strain sources, and many bacteria in fermented products, so as to improve the body's immunity and anti-stress. The effect of stimulating ability, enhancing animal digestibility and feed conversion rate, huge social and economic benefits

Inactive Publication Date: 2017-06-30
陈卫 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 1. The fermentation equipment is backward
[0009] In a sense, the conditions of production and fermentation are not standardized, so batch after batch of production, so that there are hidden dangers in product safety
The comprehensive level of fermentation technology is poor, and the fermented product has more bacteria, so when this additive is used in feed, the animal diarrhea rate is relatively high, resulting in the product not reaching the expected effect
[0010] 2. The source of bacteria is complex
[0011] The pertinence is not strong, one product is applied to many kinds of animals, which is unscientific
[0012] 3. The problem of strain inactivation
[0013] Due to the different combinations, screening, cultivation directions and methods of various microbial strains, the production level, the species of animals used, fermentation processing, storage conditions and other factors, the effects of microbial fermentation production and application are quite different, and the quality is difficult to guarantee

Method used

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  • Active-probiotic-containing feed additive as well as preparation method and application thereof
  • Active-probiotic-containing feed additive as well as preparation method and application thereof
  • Active-probiotic-containing feed additive as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 Preparation of a feed additive containing active probiotics of the present invention

[0051] 1. Strains and their sources

[0052] Lactobacillus acidophilus (CICC 22150), Lactobacillus plantarum (CICC 21794), Lactobacillus casei (CICC 23488), Lactobacillus bulgaricus (CICC 20089), Bifidobacterium infantis (CICC 21716), Bifidobacterium adolescentis (CICC 6176) , Bacillus licheniformis (CICC 20083), Bacillus subtilis (CICC 20029), Saccharomyces cerevisiae (CICC 1251), Saccharomyces cerevisiae (CICC 31105), Rhodotorula cerevisiae (CICC 31192) and Candida utilis (CICC 1422) were purchased from China Center of Industrial Culture Collection (CICC).

[0053] 2. Preparation of fermented strain seed solution

[0054] Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus casei, and Lactobacillus bulgaricus were respectively inoculated on the strain MRS plate medium, and cultured at 37°C for 48 hours.

[0055] Bifidobacterium infantis and Bifidobacteri...

Embodiment 2

[0097] Embodiment 2 Preparation of a feed additive containing active probiotics of the present invention

[0098] 1. Preparation of bacteria solution

[0099] With embodiment 1.

[0100] 2. Solid fermentation

[0101] (1) According to the volume ratio of 2:2:2:1:1:1:2:2:2:1:1:0, 20 liters of Lactobacillus acidophilus bacteria liquid (4 billion / ml), 20 liters of plant Lactobacillus liquid (2 billion / ml), 20 liters of Lactobacillus casei liquid (1 billion / ml), 10 liters of Lactobacillus bulgaricus liquid (1 billion / ml), 10 liters of Bifidobacterium infantis liquid (3 billion / ml), 10 liters of Bifidobacterium adolescence (3 billion / ml), 20 liters of Bacillus licheniformis (500 million / ml), 20 liters of Bacillus subtilis (1 billion / ml), 20 liters of wine After mixing yeast liquid (2 billion / ml), 10 liters of beer yeast liquid (2 billion / ml), and 10 liters of red yeast liquid (2 billion / ml), add 6120 liters of 40°C water and mix with 14 tons of soybean meal Put it into the fer...

Embodiment 3

[0115] Embodiment 3 Preparation of a feed additive containing active probiotics of the present invention

[0116] 1. Preparation of bacteria solution

[0117] With embodiment 1.

[0118] 2. Solid fermentation

[0119] (1) According to the volume ratio of 1:1:1:1:0:0:3:3:3:2:3:2, 10 liters of Lactobacillus acidophilus liquid (4 billion / ml), 10 liters of plant Lactobacillus liquid (2 billion / ml), 10 liters of Lactobacillus casei (1 billion / ml), 10 liters of Lactobacillus bulgaricus (1 billion / ml), 30 liters of Bacillus licheniformis (500 million / ml) ml), 30 liters of Bacillus subtilis liquid (1 billion / ml), 30 liters of Saccharomyces cerevisiae liquid (2 billion / ml), 20 liters of brewer's yeast liquid (2 billion / ml), 30 liters of Rhodotorula liquid ( 2 billion / ml) and 20 liters of Candida utilis liquid (2 billion / ml), add 7880 liters of 40°C water and 18 tons of auxiliary soybean meal, mix them into the fermentation tank, and ferment for 72-96 hours at 37°C. pool;

[0120] ...

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Abstract

The invention discloses an active-probiotic-containing feed additive as well as a preparation method and an application thereof. The active-probiotic-containing feed additive is obtained from a lactobacillus, bifidobacterium, bacillus and yeast mixed bacteria solution and auxiliaries by mixing, solid fermentation, low-temperature drying, grinding and compounding with oligosaccharide. The product contains rich bioactive substances (immune factors, organic acids, antibacterial substances and growth promotion substances), various digestive enzymes, bioactive small peptides, free amino acids and various live probiotics. After being added to feed, the feed additive can prevent and treat bacterial diarrhea and lienteric diarrhea, immunity and anti-stress capacity of animal organisms can be improved, digestibility of animals can be improved, feed conversion ratio can be increased, animal growth can be promoted, meat quality and flavor can be improved, use of drugs such as antibiotics and the like can be reduced remarkably or avoided, survival rate of the animals can be increased, production cost can be reduced, and environmental pollution can be reduced.

Description

technical field [0001] The invention relates to a feed additive, in particular to a feed additive containing active probiotics with unique efficacy, its preparation method and application. The invention belongs to the technical field of feed research. Background technique [0002] At present, people attach great importance to food safety, and many countries in the world have banned the use of antibiotics in feed. Experts pointed out that in order to fundamentally ensure food safety, only comprehensive consideration from the perspective of the food chain can be completely resolved. Therefore, people began to seek other substitutes and alternative technologies one after another. People used organic acidifiers to replace antibiotic accelerators in large quantities, and placed high hopes on them. Although acidifiers have certain effects, their use effects are limited. [0003] "Food is the most important thing for the people", food is an indispensable substance for human health...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K50/60A23K10/18A23K10/12A23K20/163A23K40/10A61K35/747A61K35/745A61K35/742A61K36/064A61K36/06A61P1/12A61P31/04A61P37/04
CPCA61K35/747A61K35/742A61K35/745A61K36/06A61K36/064A61K2300/00
Inventor 杜鹏李建贵赖水明陈伟李建秋
Owner 陈卫
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