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Japonica two-line thermo-photo-sensitive genie male sterile line seed-selection method

A nuclear sterile line and seed technology, applied in botany equipment and methods, applications, plant gene improvement, etc., can solve the problem of unstable starting temperature of fertility

Inactive Publication Date: 2017-06-30
合肥市友鑫生物技术研究中心 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, this application aims at the problem that the starting temperature of the japonica two-line CMS cannot be stabilized due to the inability to stabilize the temperature of the fertility starting point, and provides a method for breeding the two-line japonica temperature-sensitive genic male sterile line, using genetic breeding and wild rice and indica and japonica rice The factor mixing technology successfully invented the japonica two-line CMS line, and stabilized the fertility starting temperature of the japonica two-line CMS line Youxin at 23.5 degrees

Method used

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  • Japonica two-line thermo-photo-sensitive genie male sterile line seed-selection method
  • Japonica two-line thermo-photo-sensitive genie male sterile line seed-selection method
  • Japonica two-line thermo-photo-sensitive genie male sterile line seed-selection method

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Experimental program
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Effect test

Embodiment 1

[0105] The invention discloses a method for breeding a japonica two-line thermal-photosensitive genic male sterile line. The invention selects a subspecies two-line japonica-indica CMS line "Youxin 6-36S" and a japonica rice maintainer line "JDB7-82Z4" for hybridization As a pairing group, the F1 generation of (Youxin 6-36S / JDB7-82Z4) was backcrossed with the F1 generation of (X Japonica R77 / wild rice) in Hainan in the winter of the same year, and the F4 generation was recrossed with Xinhui B34Z6. Choose strong tillering ability, compact plant type, delicate plant growth, strong disease resistance, good leaf shape, green leaf color and leaf sheath, small and slightly curled leaves, round grain, large spikes and many grains, and early flowering habit. In the 8th generation of backcross JDB7-82Z4, the north-south shuttle field genetic selection was used to select a line with an earlier flowering time in addition to the above individual plant types. After 12 years and 21 generatio...

Embodiment 22015

[0187] Embodiment 2 2015 New Rice Variety Comparison Test

[0188] 1. Purpose of the test

[0189] According to the characteristics, yield and resistance of different new combinations of Youxin JD59S and different restorer lines in the previous year, it provides a scientific basis for better judging the application value of the sterile line.

[0190] 2. Experimental design

[0191] The experiment was carried out in 6 places including Fengtai, Nanling, Huainan, Lu'an, Shucheng and Xuancheng. The experimental design adopts random block arrangement, three repetitions, and a control is set for each repetition. The control variety is Tianxie No. 1, with an area of ​​0.02 mu per plot. The sowing date is May 15th, and the transplanting period is June 10th. The distance between the plants is 5×8 inches, and the two plants are planted to prevent pests and diseases.

[0192] 3. Participating combinations

[0193] A total of 7 combinations participated in the test, including 6 combin...

Embodiment 32016

[0206] Embodiment 3 2016 New Rice Variety Comparison Test

[0207] 1. Purpose of the test

[0208] According to the characteristics, yield and resistance of different new combinations of Youxin JD59S and different restorer lines in the previous year, it provides a scientific basis for better judging the application value of the sterile line.

[0209] 2. Experimental design

[0210] The experiment was carried out in 6 places including Fengtai, Nanling, Huainan, Lu'an, Shucheng and Xuancheng. The experimental design adopts random block arrangement, three repetitions, and a control is set for each repetition. The control variety is Tianxie No. 1, with an area of ​​0.02 mu per plot. The sowing date is May 15th, and the transplanting period is June 10th. The distance between the plants is 5×8 inches, and the two plants are planted to prevent pests and diseases.

[0211] 3. Participating combinations

[0212] A total of 7 combinations participated in the test, including 6 combinat...

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Abstract

The invention discloses a japonica two-line thermo-photo-sensitive genie male sterile line seed-selection method. According to the invention, a subspecies two-line japonica / indica sterile line and a rice maintainer line are subjected to hybridization matching, the two-line japonica / indica sterile line F1-generation used in winter and the rice maintainer line F1-generation are subjected to backcross at the same year, and F4-generation is subjected to composite cross of B34Z6, at the same time, through north-south shuttle selection, the single strain which has the advantages of strong tillering force, compact strain, clear plant growth, strong disease resistance, good strain leaf forms, green leaves, small leaves, slight rolling, circle grain shape, large rice ear, and early anthesis habit can be selected, backcross of JDB7-82Z4 is carried out at generation 8, field heredity selection is carried out, lines with earlier flowing time is selected, and the single strain except the above types cannot be selected, and the sterile line stable lines with a lines number 6-4 is selected through 21-generation cycle. The method solves the great economic loss for seed production-peasant household and enterprise due to uselessness by unstable fertility original temperature of the and poor seed production purity of the japonica two-line sterile line (female parent).

Description

technical field [0001] The application belongs to the field of plant breeding, and in particular relates to a breeding method for two lines of japonica thermophotosensitive male sterile lines. Background technique [0002] Rice subspecies breeding is another goal of the world's rice breeding industry. However, the japonica two-line breeding is the highest point for all rice breeders to pursue breakthrough technologies. However, it is still difficult to solve the core technologies of advancing the flowering time of the japonica two-line male sterile line. [0003] The best fertilization ability of rice pollen under natural conditions is within 5-8 minutes after flowering, which solves the problem that the flowering time of the Japonica two-line male sterile line (female parent) is late in the world and the flowering time of the sub-recovery line (male parent) is different. Very long (1.5-2 hour) can not meet (japonica does not indica recover), causes the per mu output of lar...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 王文余陈海洲张华胜吴文革陈刚
Owner 合肥市友鑫生物技术研究中心
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