Japonica two-line thermo-photo-sensitive genie male sterile line seed-selection method
A nuclear sterile line and seed technology, applied in botany equipment and methods, applications, plant gene improvement, etc., can solve the problem of unstable starting temperature of fertility
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Embodiment 1
[0105] The invention discloses a method for breeding a japonica two-line thermal-photosensitive genic male sterile line. The invention selects a subspecies two-line japonica-indica CMS line "Youxin 6-36S" and a japonica rice maintainer line "JDB7-82Z4" for hybridization As a pairing group, the F1 generation of (Youxin 6-36S / JDB7-82Z4) was backcrossed with the F1 generation of (X Japonica R77 / wild rice) in Hainan in the winter of the same year, and the F4 generation was recrossed with Xinhui B34Z6. Choose strong tillering ability, compact plant type, delicate plant growth, strong disease resistance, good leaf shape, green leaf color and leaf sheath, small and slightly curled leaves, round grain, large spikes and many grains, and early flowering habit. In the 8th generation of backcross JDB7-82Z4, the north-south shuttle field genetic selection was used to select a line with an earlier flowering time in addition to the above individual plant types. After 12 years and 21 generatio...
Embodiment 22015
[0187] Embodiment 2 2015 New Rice Variety Comparison Test
[0188] 1. Purpose of the test
[0189] According to the characteristics, yield and resistance of different new combinations of Youxin JD59S and different restorer lines in the previous year, it provides a scientific basis for better judging the application value of the sterile line.
[0190] 2. Experimental design
[0191] The experiment was carried out in 6 places including Fengtai, Nanling, Huainan, Lu'an, Shucheng and Xuancheng. The experimental design adopts random block arrangement, three repetitions, and a control is set for each repetition. The control variety is Tianxie No. 1, with an area of 0.02 mu per plot. The sowing date is May 15th, and the transplanting period is June 10th. The distance between the plants is 5×8 inches, and the two plants are planted to prevent pests and diseases.
[0192] 3. Participating combinations
[0193] A total of 7 combinations participated in the test, including 6 combin...
Embodiment 32016
[0206] Embodiment 3 2016 New Rice Variety Comparison Test
[0207] 1. Purpose of the test
[0208] According to the characteristics, yield and resistance of different new combinations of Youxin JD59S and different restorer lines in the previous year, it provides a scientific basis for better judging the application value of the sterile line.
[0209] 2. Experimental design
[0210] The experiment was carried out in 6 places including Fengtai, Nanling, Huainan, Lu'an, Shucheng and Xuancheng. The experimental design adopts random block arrangement, three repetitions, and a control is set for each repetition. The control variety is Tianxie No. 1, with an area of 0.02 mu per plot. The sowing date is May 15th, and the transplanting period is June 10th. The distance between the plants is 5×8 inches, and the two plants are planted to prevent pests and diseases.
[0211] 3. Participating combinations
[0212] A total of 7 combinations participated in the test, including 6 combinat...
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