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Breeding method of three-line male sterile line with erect panicle, long stigma and long panicle neck

A three-line sterile line, long panicle neck technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of decreased seed viability, environmental pollution, increased cost, etc., and improved seed production yield. , the effect of reducing production costs and increasing economic benefits

Active Publication Date: 2016-06-08
湖南神农大丰种业科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the male sterile line of the three-line hybrid rice currently popularized and applied in production has a serious phenomenon of neck wrapping and ear wrapping. In seed production, gibberellin (commonly known as 920) needs to be sprayed to solve the ear wrapping problem. However, spraying gibberellin is not only Increased the cost of seed production, but also caused environmental pollution and reduced seed viability, while the long-neck CMS line containing the eui gene can achieve genetic removal of the neck-wrapped ear, and the CMS line for seed production can use no or less red Mycin, so that the cost of hybrid rice seed production is reduced, seed quality is improved and environmental pollution is reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Choose the erect panicle rice variety Shennong 265 (A), the long stigma rice variety Hai B (B), and the long panicle rice variety IR68902eB1 (C) to provide parents for the target traits, and Longtepu B recurrent parents (R) for Hybrid A / B, get hybrid F 1 Substituting F 1-AB Means

[0019] 2) Plant hybrid F 1 Generation, cross F at flowering 1-AB / C, get F 1 , With F 1-ABC Means

[0020] 3) Plant F 1-ABC , F 2-ABC , Plant F 2-ABC , A population of 1800 plants, the plants with the traits of erect panicle, long stigma and long panicle neck were selected at the heading and flowering stage, and the individual plants were harvested at the maturity stage (F 3-ABC );

[0021] 4) Strain planting 3) F obtained with the traits of erect panicle, long stigma and long panicle neck at the same time 3-ABC Plant 30 plants for each line. At the heading and flowering stage, 2 lines with upright panicle, long stigma, and long panicle neck were screened and the traits were not segregated....

Embodiment 2

[0029] 1) Select the erect panicle rice variety Shennong 265 (A), the long stigma rice variety Hai B (B), and the long panicle rice variety IR68902eB1 (C) as the parents for the target traits, and the flower 2B as the recurrent parent (R) for crossbreeding A / B, get hybrid F 1 Substituting F 1-AB Means

[0030] 2) Plant hybrid F 1 Generation, cross F at flowering 1-AB / C, get F 1 , With F 1-ABC Means

[0031] 3) Plant F 1-ABC , F 2-ABC , Plant F 2-ABC , A population of 1900 plants, the plants with the traits of erect panicle, long stigma and long panicle neck were selected at the heading and flowering stage, and each plant was harvested at the maturity stage (F 3-ABC );

[0032] 4) Strain planting 3) F obtained with the traits of erect panicle, long stigma and long panicle neck at the same time 3-ABC Plant 30 plants for each line. At the heading and flowering stage, select 3 lines with upright panicle, long stigma and long panicle neck without segregation of the traits, and sele...

Embodiment 3

[0040] 1) Select the erect panicle rice variety Shennong 265 (A), the long stigma rice variety Hai B (B), and the long panicle rice variety IR68902eB1 (C) as the parents to provide the target traits, and Fujian standard 1B as the recurrent parent (R). Hybrid A / B, get hybrid F 1 Substituting F 1-AB Means

[0041] 2) Plant hybrid F 1 Generation, cross F at flowering 1-AB / C, get F 1 , With F 1-ABC Means

[0042] 3) Plant F 1-ABC , F 2-ABC , Plant F 2-ABC , A population of 2000 plants, at the heading and flowering stage, the plants with the traits of erect panicle, long stigma and long panicle neck were selected for listing, and individual plants were harvested at maturity stage (F 3-ABC );

[0043] 4) Strain planting 3) F obtained with the traits of erect panicle, long stigma and long panicle neck at the same time 3-ABC Plant 30 plants for each line. At the heading and flowering stage, select 3 lines with upright panicle, long stigma and long panicle neck without segregation of t...

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Abstract

The invention discloses a breeding method of an erect panicle type long stigma and elongated upmost internode three-line sterile line, belonging to the technical field of rice sterile line breeding. According to the method, an erect panicle type rice variety Shennong 265, a long stigma rice variety Hai B and an elongated upmost internode rice variety IR68902eB1 are taken as target trait providing parents, a superior three-line maintenance line which is large in popularization area in production is taken as a recurrent parent, and the three-line sterile line which has the characters including erect panicle, long stigma and elongated upmost internode at the same time can be bred by hybridization, multiple-crossing and backcross transformation, and the agronomic trait and the yield trait of the three-line sterile line are close to those of the original sterile line. The three-line sterile line does not have sheathed panicle, and is high in stigma exposure rate and outcrossing rate; after three-line hybrid rice is prepared by the three-line sterile line, the seed production yield of the hybrid rice is improved, the production cost is lowered, and the environmental pollution is reduced.

Description

Technical field [0001] The invention specifically relates to a method for breeding three-line sterile lines with erect ears, long stigma and long ear necks, and belongs to the technical field of rice sterile line breeding. Background technique [0002] The breeding of dwarf stalks and the breeding of hybrid rice are two major leaps in my country's rice science and technology revolution, which has greatly improved my country's rice output and basically solved the problem of food and clothing for the people across the country. However, the sterile line of the three-line hybrid rice currently popularized and applied in production has serious neck and ear covering. In seed production, spraying gibberellin (commonly known as 920) is needed to solve the problem of ear covering. However, spraying gibberellin not only Increasing the cost of seed production, but also causing environmental pollution and decreased seed viability, and the long ear neck sterile line containing eui gene can ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 章清杞陈幼玉王和寿倪济民李晓燕
Owner 湖南神农大丰种业科技有限责任公司
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