Method for promoting anthesis of Indica sterile line

Abstract

The invention discloses a method for promoting anthesis of an Indica sterile line. The method comprises the following steps: selecting single strains which are in a full heading stage and have neat layers of ears of rice in the sterile line in an afternoon of a day before the day when test crossing and transformation are carried out, cutting out old and excessively young ears of rice in the single strains of the sterile line, retaining flag leaves of the ears of rice, stripping the flag leaves down to ear necks, cutting out bloomed glumous flowers at upper parts of the ears of rice and glumous flowers with whitening glumous hulls at the basal parts of the ears of rice, carrying out watering and moisture retention after trimming and standing the single strains of the sterile line for subsequent usage; and in the day of test crossing and transformation, treating the sterile line in lukewarm water with a temperature of 44 to 46 DEG C, then wrapping the ears of rice of the sterile line with a wet agricultural black mulch film immediately, placing the wrapped ears of rice in sunlight for treatment for 20 to 30 min, opening the wrapping mulch film, flicking off unnecessary moisture and anther, covering the ears of rice with a waterproof hybridization paper bag, enclosing the paper bag and carrying out further test crossing and transformation. With a technical scheme in the invention, anthesis of the sterile line is promoted, an outcrossing rate is increased, seed quality is improved and the seedling rate of a coeno-species is enhanced.

Description

technical field [0001] The invention relates to a highly efficient method for sexual hybridization of indica-type sterile lines, more specifically relates to a method for promoting flowering of indica-type sterile lines, which belongs to the category of sexual hybridization of rice. Background technique [0002] Rice is the largest crop in my country. Hybrid indica rice has high yield, wide adaptability, and is easy to cultivate. The planting area accounts for 50% of the total rice area in the country, and the output accounts for 60% of the total rice output. made a great contribution. The improvement of hybrid rice varieties has always been the key field of agricultural research in my country. High quality, multiple resistance and high yield are the long-term goals of hybrid rice breeding in my country. [0003] The heterosis of rice is based on the sexual hybridization of cytoplasmic male sterile lines (hereinafter referred to as male sterile lines) and related restorer li...

AI Technical Summary

Problems solved by technology

The inadequacies of the "grave clipping" method are mainly manifested in: 1) Higher requirements on glume clipping technology: too much clipping glumes can easily damage the stigma; Seeds produced by glumes are exposed to the air, and are easy to be eaten by various pests and mildew, and the quality of the seeds is difficult to guarantee; 3) The seedling rate of clipped glume seeds after sowing is greatly affected by environmental conditions, and rotten seeds are prone to occur in the field. The seedling rate is generally low; 4) Clipping glumes is time-consuming and inefficient, which limits the improvement of breeding efficiency