Multi-channel solid-phase polypeptide synthesis method

Abstract

The invention discloses a multi-channel solid-phase polypeptide synthesis method. The multi-channel solid-phase polypeptide synthesis method comprises the following steps: 1, connecting; 2, charging resin respectively; 3, swelling the resin; 4, performing a deprotection reaction; 5, washing the resin; 6, detecting whether the deprotection reaction is completed or not; 7, performing a coupling reaction; and 8, detecting whether the coupling reaction is completed or not. During using, polypeptide synthesis tubes are grouped according to coupling solutions needed in a next reaction step, and the polypeptide synthesis tubes needing the same coupling solutions are grouped into the same group, so that a basis is provided for achieving a batch reaction; the invention further designs a technical scheme in which a plurality of polypeptide synthesis tubes correspond to the same reaction cylinder. Compared with the conventional synthesis device and the conventional synthesis method, the multi-channel solid-phase polypeptide synthesis method has the significant advantage of high efficiency, so that the efficiency of seeking for a polypeptide substance with the best effect is improved.

Description

technical field [0001] The invention relates to the field of biochemical experiments, in particular to a polypeptide solid-phase synthesis method. Background technique [0002] Polypeptide solid-phase synthesis is to connect the protected amino acid to a solid resin, remove the protective group, then react the next protected amino acid with the residue of the first amino acid and form a peptide bond, and then remove the protective group of the second amino acid group, and then connect to the next amino acid, so that the cycle continues to increase the length of the peptide chain. In the solid-phase synthesis of polypeptides, solid resin is the necessary reaction substrate. [0003] In the polypeptide solid-phase synthesis reaction, a suction filtration device is required, and the suction filtration device includes a suction filtration bottle and a vacuum pump connected with the suction filtration bottle. [0004] In the solid-phase synthesis of polypeptides, the reaction s...

AI Technical Summary

Problems solved by technology

When preparing multiple derivative peptides based on one parent peptide, most of the coupling solutions required for various derivative peptides are the same, but the current solid-phase peptide synthesis equipment requires the same coupling solution for the preparation of each derivative peptide. Coupling reactions are performed separately when preparing different derivative peptides, and there is a lack of devices and methods that can perform peptide solid-phase synthesis reactions in batches

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