Composition for detecting colla corii asini content in compound colla corii asini preparation, kit and detection method thereof
A compound donkey-hide gelatin and composition technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of quantitative detection of donkey skin source components, discounts on accuracy and reliability, and supervision of unfavorable market donkey-hide gelatin products.
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Embodiment 1
[0033] 1Materials and reagents
[0034] 1) Material
[0035] A variety of pure glues, including donkey skin, horse skin glue, yellow gelatin, pig skin glue, sheep skin glue, fish skin glue (reference test substrate), respectively, donkey skin, horse skin, cowhide, pig skin, sheep skin and fish skin It is obtained after decoction, concentration and drying.
[0036] Polypeptide I (shown in SEQ ID NO. 1), polypeptide II (shown in SEQ ID NO. 2), and polypeptide III (shown in SEQ ID NO. 3) were synthesized by a biological company.
[0037] Compound donkey-hide gelatin preparation sample: extract the concentrated solution of plant medicinal materials in the production process of compound donkey-hide gelatin, respectively add the above pure product gum, and add 1.0g pure product gum sample for every 9g of plant medicinal material extraction concentrated solution to make compound donkey-hide gelatin paste containing donkey-hide gelatin ( Preparation 1), fake donkey-hide gelatin containing ho...
Embodiment 2
[0057] 1Materials and reagents
[0058] 1) Material
[0059] Polypeptide I (shown in SEQ ID NO. 1), polypeptide II (shown in SEQ ID NO. 2), and polypeptide III (shown in SEQ ID NO. 3) were synthesized by a biological company.
[0060] The mixed gum samples were prepared by adding different quality donkey-hide gelatin samples to the pure product gum samples in Example 1, including yellow gelatin containing 15% donkey-hide gelatin, yellow gelatin containing 30% donkey-hide gelatin, and yellow gelatin containing 60% donkey-hide gelatin.
[0061] Compound donkey-hide gelatin preparation samples: extract the concentrated solution of plant medicinal materials in the production process of compound donkey-hide gelatin slurry, add the above mixed gum samples respectively, and add 1.0g mixed gelatin sample to each 9g plant medicinal extract concentrated solution to make 3 fake compound donkey-hide gelatin slurry. They were named Formulation 1, Formulation 2, and Formulation 3. The donkey-hide g...
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