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Composition and kit for detecting content of donkey hide source component in donkey-hide gelatin, and detection method thereof

A composition and donkey skin technology, which are applied in the field of compositions and kits for detecting the content of donkey skin source components in donkey hide gelatin, can solve the problem of quantitative detection of donkey skin source components, discounts on accuracy and reliability, and supervision of unfavorable market donkey hide gelatin products, etc. question

Active Publication Date: 2017-06-13
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The two reported methods are unable to carry out more accurate quantitative detection of donkey-hide gelatin-derived components, but to determine the authenticity of donkey-hide gelatin by detecting whether donkey-hide gelatin contains donkey components and excluding other animal-derived components; but the donkey-hide gelatin Adulteration may be varied, so there will be a variety of animal-derived ingredients that need to be excluded. There are important flaws in using the exclusion method to identify the authenticity of donkey-hide gelatin, and its accuracy and reliability will be greatly reduced. This situation is very unfavorable to the market for donkey-hide gelatin. product regulation

Method used

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  • Composition and kit for detecting content of donkey hide source component in donkey-hide gelatin, and detection method thereof
  • Composition and kit for detecting content of donkey hide source component in donkey-hide gelatin, and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]The detection of embodiment 1 pure product glue

[0033] 1. Materials and reagents

[0034] Materials: All kinds of pure glue, including donkey-hide gelatin, horsehide glue, yellow gelatin, pigskin glue, sheepskin glue, fish skin glue (the reference substance detection matrix), donkey skin, horse hide, cowhide, pigskin, sheepskin and Obtained by decocting fish skin.

[0035] Polypeptide I (shown in SEQ ID NO.1), polypeptide II (shown in SEQ ID NO.2), and polypeptide III (shown in SEQ ID NO.3) were synthesized by a biological company.

[0036] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure)

[0037] 2. Detection method

[0038] 1) Preparation of matrix solution: Weigh 0.50 g of reference substance detection matrix in a 250ml measuring bottle, add a small amount of 1% NH 4 HCO 3 solution (pH8.0), ultrasonically dissolve the sample completely, and use 1% NH 4 HCO 3 Solution (pH8.0) diluted to the mark, shake well.

[0039] 2) Preparation of t...

Embodiment 2

[0053] The detection of embodiment 2 mixed rubber samples

[0054] 1. Materials and reagents

[0055] Materials: the mixed gum sample is made by adding different quality donkey-hide gelatin to the pure product gum sample in Example 1, including yellow gelatin containing 15% (w / w) donkey-hide gelatin, yellow gelatin containing 30% (w / w) donkey-hide gelatin , yellow gelatin containing 60% (w / w) donkey-hide gelatin, horse hide glue containing 30% (w / w) donkey-hide gelatin, and pigskin glue containing 30% (w / w) donkey-hide gelatin, respectively as samples 1-5.

[0056] Polypeptide I (shown in SEQ ID NO.1, purity 99.2%), polypeptide II (shown in SEQ ID NO.2, purity 99.5%), polypeptide III (shown in SEQ ID NO.3, purity 99.2%), delivered to Biological company synthesis.

[0057] Reagents: ammonium bicarbonate (analytical pure), trypsin (sequential pure).

[0058] 2. Detection method

[0059] 1) Preparation of matrix solution: Weigh 0.50 g of reference substance detection matrix i...

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Abstract

The invention discloses a composition and a kit for detecting the content of donkey hide source component in donkey-hide gelatin, and a detection method thereof. The composition comprises polypeptide I, polypeptide II, polypeptide III and control detection matrix, wherein the amino acid sequence of the polypeptide I is shown as SEQ ID NO.1, the amino acid sequence of the polypeptide II is shown as SEQ ID NO.2, the amino acid sequence of the polypeptide III is shown as SEQ ID NO.3, and the control detection matrix is fishskin gelatin. The detection value of the polypeptide II is subtracted from the detection value of the polypeptide I, and the detection deviation can be corrected by utilizing the detection value of the polypeptide III, so that the detection of content of donkey hide source component in the donkey-hide gelatin can be realized. The method has the advantages of being accurate in content detection, and simple to operate, and has wide application prospects on the aspect of detecting the donkey hide source component in the donkey-hide gelatin.

Description

technical field [0001] The invention relates to a composition and a kit for detecting the content of donkey-hide-derived components in donkey-hide gelatin, and also includes a method for detecting the content of donkey-hide-derived components in donkey-hide gelatin by using the composition or the kit. The invention belongs to the technical field of medicine and food detection . Background technique [0002] Ejiao, as a traditional precious Chinese medicine, has been eaten for thousands of years. It has the functions of nourishing blood, nourishing yin, moistening dryness and stopping bleeding. The Chinese Pharmacopoeia stipulates that donkey-hide gelatin is a solid gelatin obtained by decocting and concentrating the dried skin or fresh skin of Equus asinus L., an equine animal donkey. Pure donkey-hide gelatin must come from 100% donkey skin to ensure the efficacy of the product. From the perspective of raw material taxonomy, donkeys belong to the genus Equus of the order P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 郝向慧郭尚伟段小波徐云鹏王静王玉娇石永坚庞慧慧周祥山
Owner SHAN DONG DONG E E JIAO
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