An integrated microfluidic chip system for crop disease spore detection

Abstract

The invention discloses an integrated micro-fluidic chip system used for crop disease spore detection. A feeding channel, a gas mixing channel, an isolated spore separation channel and a isolated spore enrichment plate of a micro-fluidic chip are connected in sequence from left to right; the front side of the inlet end of the isolated spore separation channel is provided with a constant current purification channel communicated with external airflow; the contour line of the horizontal cross section of the rear side wall of the isolated spore separation channel is in a parabola shape; the front side wall of the isolated spore separation channel is in a flat plate shape; the airflow in the constant current purification channel acts on the inlet end of the isolated spore separation channel; spores of different qualities carry out the parabola-shaped movement of different trajectories to the rear side wall of the isolated spore separation channel at different accelerated speeds under the same constant force function; the top in a magazine is provided with a ball lead screw arranged along a front and back direction; the ball lead screw is fixedly connected with a camera through the lower end of a nut assembly; the camera is suspended just above the isolated spore enrichment plate, and quick detection and analysis can be carried out on the field of airborne fungi.

Description

technical field [0001] The invention relates to the fields of crop disease detection and microfluidic detection, in particular to a microfluidic chip system for crop disease spore detection. Background technique [0002] Crop fungal diseases and bacterial diseases are pathogenic bacteria that cause crop tissue necrosis, rot, wilting, burns, ulcers, tissue hyperplasia and other disease symptoms. Fungal spores are the main reproductive organs of fungi. Under suitable conditions, spores germinate and form hyphae for division and reproduction. When the external environment is not suitable, they can live in a dormant state for a long time; bacterial spores are produced by bacteria in harsh environments. Bacterial spores are highly transmissible due to their high environmental resistance, and when the environment is right, the bacterial spores germinate. Therefore, detecting the species and content of zoospores in the air can provide basic data for predicting crop diseases. [0...

AI Technical Summary

Problems solved by technology

The Chinese invention patent application number is 201510275959.0, which discloses a microfluidic chip for capturing fungal spores in the air, which is characterized in that the chip contains gas flow channels and spore retention channels, arranged radially and converging in the center of the chip The pumping hole is connected with a reciprocating pump, and can be combined with a microfluidic chip for molecular biology analysis; however, the separation of spore species is cumbersome, and a separate culture medium is required to elute the fungal spores in the chip pipeline, making subsequent data more difficult to deal with

Images