Method for extracting pectin from pear peel

A pear peel and pectin technology, which is applied in the field of pectin extraction from pear peel, can solve the problems of difficult temperature control, etc., and achieve the effects of easy operation, shortened crushing time, and improved extraction rate

Inactive Publication Date: 2017-06-20
CHONGQING DOUHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, there have been researches on the technology of extracting pectin from pumpkin by microwave-assisted acid extraction, but it is difficult to control the temperature in the operation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting pectin from pear peel, the steps are as follows:

[0023] (1) Pretreatment: Wash the pear skin, drain the water, dry it at 60-80°C, then cut it into small pieces, crush it with a pulverizer, and pass through a 40-mesh sieve to get the pear skin powder for later use;

[0024] (2) Extraction:

[0025] Enzyme treatment: Take 100g of pear peel powder in step (1), add 800ml of pure water, adjust its temperature to 30°C, pH to 5.5, add 1 g of a compound composed of cellulase and acid protease (1:1) Enzyme, enzymatic hydrolysis for 2 hours, to obtain enzymatic hydrolyzate;

[0026] Ultrasonic treatment: take the enzymatic hydrolysis solution and adjust the pH to 1.00 with hydrochloric acid, heat up to 80°C, and ultrasonicate for 80 minutes at a power of 175W;

[0027] (3) Decolorization: add activated carbon to the ultrasonic treatment solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:100...

example 2

[0031] A method for extracting pectin from pear peel, the steps are as follows:

[0032] (1) Pretreatment: Wash the pear skin, drain the water, dry it at 60-80°C, then cut it into small pieces, crush it with a pulverizer, and pass through a 40-mesh sieve to get the pear skin powder for later use;

[0033] (2) Extraction:

[0034] Enzyme treatment: Take 100g of pear peel powder in step (1), add 800ml of pure water, adjust its temperature to 30°C, pH to 6.5, add 3g of compound enzyme composed of cellulase and acid protease (4:1) , enzymatically hydrolyzed for 2 hours to obtain enzymolyzate;

[0035] Ultrasonic treatment: take the enzymatic hydrolysis solution and adjust the pH to 1.50 with hydrochloric acid, heat up to 90°C, and ultrasonicate for 70 minutes at a power of 175W;

[0036] (3) Decolorization: add activated carbon to the ultrasonic treatment solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:150mL, trea...

Embodiment 3

[0040] A method for extracting pectin from pear peel, the steps are as follows:

[0041] (1) Pretreatment: Wash the pear skin, drain the water, dry it at 60-80°C, then cut it into small pieces, crush it with a pulverizer, and pass through a 40-mesh sieve to get the pear skin powder for later use;

[0042] (2) Extraction:

[0043] Enzyme treatment: Take 100g of pear peel powder in step (1), add 600ml of pure water, adjust its temperature to 45°C, pH to 6.0, add 2g of compound enzyme composed of cellulase and acid protease (2.5:1) , enzymatically hydrolyzed for 2 hours to obtain enzymolyzate;

[0044] Ultrasonic treatment: take the enzymatic hydrolysis solution and adjust the pH to 1.25 with hydrochloric acid, raise the temperature to 85°C, and ultrasonicate for 75 minutes at a power of 175W;

[0045] (3) Decolorization: add activated carbon to the ultrasonic treatment solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution i...

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PUM

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Abstract

The invention discloses a method for extracting pectin from pear peel. The method adopting the pear peel as a raw material concretely comprises the following steps: 1, preprocessing; 2, enzymatic hydrolysis and ultrasonic treatment; 3, decolorizing; and 4, drying for obtaining the pectin. The pectin is extracted from the pear peel under the combined action of a compound enzyme and ultrasonic waves, the enzyme action condition is mild, and the compound enzyme also has a wall breaking effect to make the pectin in the pear peel thoroughly leached; and the ultrasonic extraction process is a physical process, no chemical reactions occur in the extraction process, the extraction rate is high, functional components of extract are effectively protected, the breaking time is shortened, the pectin content of the pear peel is greater than 70%, and the impurity content of the pectin is greatly reduced. The above preparation method adopting the pear peel as a raw material has the advantages of simplicity, high yield, and realization of good quality of the produced pectin, so the purpose of changing wastes into valuables is realized.

Description

technical field [0001] The invention relates to a method for extracting pectin, in particular to a method for extracting pectin from pear peel. Background technique [0002] Existing pectin extraction mostly adopts alcohol extraction, the consumption of ethanol is large and the cost is high, and the obtained pectin powder has high impurity content. The traditional heating extraction method requires high temperature and a long time, and the pectin in the raw material will inevitably be denatured and decomposed. [0003] In recent years, there have been researches on the technology of extracting pectin from pumpkin by microwave-assisted acid extraction, but it is difficult to control the temperature in the operation. Ultrasonic extraction technology is a new method applied to the extraction of active ingredients, which has the advantages of mild extraction conditions, low energy consumption, and high efficiency. [0004] The theoretical basis for the application of ultrasoun...

Claims

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Application Information

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IPC IPC(8): C08B37/06
CPCC08B37/0045
Inventor 万雪峰其他发明人请求不公开姓名
Owner CHONGQING DOUHAO BIOTECH
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