Traditional Chinese medicine anti-corrosion preservative as well as preparation method and application thereof
A technology of antistaling agent and traditional Chinese medicine, applied in the field of antistaling agents, can solve the problems of inability to eat directly, high price, peculiar smell, etc., achieve remarkable freshness preservation effect, reduce bad fruit rate, and prevent water loss
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[0041] A kind of preparation method of traditional Chinese medicine antistaling agent of the present invention, comprises the following steps:
[0042] Step 1, extraction of clove volatile oil
[0043] According to the consumption ratio of cloves: water=1g:6ml, after cloves are mixed with water, be placed in the volatile oil extraction device, extract 3.5h~4.5h under the condition of 100 watts, collect cloves volatile oil;
[0044] Step 2, extraction of cinnamon volatile oil
[0045] According to the dosage ratio of cassia bark: water=1g:6ml, the cassia bark is mixed with water and placed in the volatile oil extraction device, extracted under 100 watts for 3.5h~4.5h, and the cassia bark volatile oil is collected;
[0046] Step 3, preparation of volatile oil compound solution
[0047] According to the dosage ratio of clove volatile oil, cinnamon volatile oil, chitosan, citric acid, ethanol aqueous solution is (15~35)ml:(150~250)ml:(0.5~2)g:(5~15)g:1000ml, Firstly mix citric ...
Embodiment 1
[0055] The screening of embodiment 1 Chinese medicine
[0056] 1. Determination of the bacteria to be tested: By consulting the literature, the common bacteria on fruits and vegetables were selected, namely Escherichia coli, Staphylococcus aureus and Bacillus subtilis.
[0057] 2. Activation of test bacteria: After activating the test bacteria (Escherichia coli, Staphylococcus aureus and Bacillus subtilis) with LB medium respectively, select a single colony to inoculate in the liquid medium, and after cultivating on a shaker for 6 hours, Make the OD value around 1.0 and set aside.
[0058] 3. By consulting the literature, preliminarily select traditional Chinese medicines with antibacterial ability, namely clove, cinnamon bark, wild chrysanthemum, dried ginger and mint.
[0059] 4. Determination of the minimum inhibitory concentration of volatile oil
[0060] (1) Extraction of clove volatile oil: take by weighing 50g of Chinese medicine clove, add in volatile oil extraction ...
Embodiment 2
[0071] The selection of the optimum proportioning ratio of each component of embodiment 2
[0072] 1. Extraction of clove volatile oil
[0073] According to the dosage ratio of cloves: water=1g:6ml, cloves and water are mixed and placed in a volatile oil extraction device, extracted under 100 watts for 3.5h to 4.5h, and cloves volatile oil is collected; similarly, the extraction process of cinnamon volatile oil is the same as above.
[0074] 2. Preparation of volatile oil compound liquid
[0075] Take by weighing 10g citric acid, add 1000ml ethanol aqueous solution (the volume fraction of ethanol in the ethanol aqueous solution is 75%) and mix, according to the ratio given in table 1, add chitosan, clove volatile oil, cassia bark volatile oil successively, mix evenly, obtain Multi-group volatile oil compound solution, sealed and placed at room temperature for 24 hours, and set aside.
[0076] 3. Orthogonal experiment: select three factors and three levels through the pre-exp...
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