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A high-throughput pattern-induced cell screening chip

A cell-inducing, high-throughput technology, applied in measurement devices, instruments, biomass post-processing, etc., can solve problems such as harshness and complex patterns, and achieve the effect of ensuring reliability and enhancing the culture system

Active Publication Date: 2018-10-23
重庆艾令达生物医学研究有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many experiments on the differentiation and proliferation of various types of stem cells with various patterns, but the patterns for such experiments are relatively complicated, and the requirements for the same experimental conditions in cell comparison experiments are also very strict, so it is necessary to have an easy-to-process Equipment for such experiments

Method used

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  • A high-throughput pattern-induced cell screening chip
  • A high-throughput pattern-induced cell screening chip

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Experimental program
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specific Embodiment 1

[0015] refer to Figure 1 to Figure 2 Chip-shaped structure, the chip has two levels of culture chambers 22 . The first-stage circular cultivation chamber 12 and the second-stage circular cultivation chamber 15, as well as various channels 24, have all been treated with hydrophobic treatment. The first-stage circular culture chamber is left-right symmetrical, and the bottom of the culture chamber is processed with evenly distributed large circular cell pits 4 and small circular cell pits 13 . Two pairs of 4 second-level circular culture chambers process a pair of large triangular cell pits 16 and small triangular cell pits 17, and another pair of large rectangular cell pits 2 and small rectangular cell pits 3. The bottom surface of the cell well 23 is treated with hydrophilicity and coated with FN protein.

[0016] All channels: width = 150 µm, height = 100 µm; all circular culture chambers 22 diameter ϕ = 10 mm; first stage: cell pit 23 radius r = 65 µm, depth = 50 µm in la...

specific Embodiment 2

[0018] refer to Figure 1 to Figure 2 Chip-shaped structure, the chip has three levels of cultivation chambers 22 . The first level is a pair of 2 square culture chambers 22 with side length=5mm, the second level is 4 round culture chambers 22 with ϕ=5mm, and the third level is four pairs with a total of 8 diamond-shaped culture chambers 22 with side length=5mm. And all kinds of channels 24 have been subjected to hydrophobic treatment. The bottom of the first-stage square culture chamber is processed with evenly distributed large circular cell pits 4 and small circular cell pits 13 . Two pairs of 4 second-level circular culture chambers 22 are used for processing the large triangular cell pit 16 and the small triangular cell pit 17, and the other pair are for processing the large rectangular cell pit 2 and the small rectangular cell pit 3. Four pairs of 8 third-level 8 diamond-shaped culture chambers 22, respectively large / small diamond-shaped cell pits 23, large / small squar...

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Abstract

The invention provides a high-throughput pattern-induced cell screening chip. The high-throughput pattern-induced cell screening chip is characterized in that the chip is rectangular; four injection holes are processed in a rectangular short-edge tip; paired culture chambers are processed in the rectangular short-edge tip; and paired cell pits which are same in shape and different in size are uniformly distributed and processed at the bottom of each culture chamber. The high-throughput pattern-induced cell screening chip has the beneficial effects that the chip can be flexibly provided with more channels and culture chambers according to the experiment requirements, and a culture system can be strengthened. Multiple indexes can be detected in the limited area of the chip, thereby achieving timely and effective micro-detection. Real-time monitoring can be carried out in the detection time period, nutrient substances are controlled or the input dose of a chemical is inspected, and the detection reliability is ensured, so that the result can reflect a real cell growth environment to achieve the eventually needed detection target.

Description

technical field [0001] The invention relates to a cell culture biochip for cell screening test, which is a high-throughput pattern-induced cell screening chip in terms of use. Background technique [0002] Biochip is a high-tech that has developed rapidly in the field of biomedical engineering in the past 20 years. Because of its high-throughput, miniaturization and automation characteristics, it conforms to the principles of fast, efficient, and low-cost industrial operation, and has very strong application prospects in the fields of disease diagnosis, drug screening, and cell culture. At present, there are many experiments on the differentiation and proliferation of various types of stem cells with various patterns, but the patterns for such experiments are relatively complicated, and the requirements for the same experimental conditions in cell comparison experiments are also very strict, so it is necessary to have an easy-to-process equipment for such experiments. The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCG01N33/5008
Inventor 廖晓玲徐文峰徐紫宸解文月
Owner 重庆艾令达生物医学研究有限公司
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