Method for using biochip for cell fluid experiment
A technology for biochips and experiments, applied in biochemical equipment and methods, tissue cell/virus culture devices, biochemical instruments, etc., can solve the problems of lack of cell culture biochips, etc., to reduce experimental time and errors, and micro-observation , The effect of ensuring reliability
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Embodiment 1
[0018] In the first step, the selected chip shape specifications are as follows: the sides of culture area 13 are composed of arc walls with R=0.8mm in reverse series, and the sides of culture area 27 are made of arc walls with R=4mm. Arc-concave-convex chips formed in series. The length L of the cultivation zone 13 and the cultivation zone 27 is 25mm, the widest dimension D of the cultivation zone 13 and the cultivation zone 27 is 10mm, and the width dimension of the connecting channel is d=1mm. The experimental fluid injection flow rate was 15 μL / S and 30 μL / S. Input the shape and size of the selected rectangular culture area 3 and conical culture area 7 of the chip, as well as the dimensions of all the channels into the computer, and use CAD software to draw; then import the drawing data into the fluid simulation software, and calculate the experimental fluid flow rate. The fluid flow network of culture zone one 3 and culture zone two 7.
[0019] In the second step, use m...
Embodiment 2
[0024] In the first step, the selected chip shape specifications are as follows: the side length of the culture area-3-pointed cone is 0.5mm, and the convex-concave angle between the two sides is α 1 =30° sharp angle. The side length of the pointed cone in culture area 27 is 4mm, and the convex-concave angle between the two sides is α 2 =80° sharp angle. The length L of the cultivation zone 13 and the cultivation zone 27 is 40mm, the widest dimension D of the cultivation zone 13 and the cultivation zone 27 is 30mm, and the width dimension d of the connecting channel is 2.5mm. The experimental fluid injection flow rate was 25 μL / S and 50 μL / S. Input the shape and size of the selected rectangular culture area 3 and conical culture area 7 of the chip, as well as the dimensions of all the channels into the computer, and use CAD software to draw; then import the drawing data into the fluid simulation software, and calculate the experimental fluid flow rate. The fluid flow networ...
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