Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for using biochip for cell fluid experiment

A technology for biochips and experiments, applied in biochemical equipment and methods, tissue cell/virus culture devices, biochemical instruments, etc., can solve the problems of lack of cell culture biochips, etc., to reduce experimental time and errors, and micro-observation , The effect of ensuring reliability

Active Publication Date: 2018-10-23
徐州龙肽医学检验实验室有限公司 +2
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the study of cell differentiation and proliferation, cell mechanics experiments are often used to simulate in vivo fluids, and one of the effects of cell exploration on different shear forces is one of them, but there is currently a lack of cell culture biochips for shear force experiments , has achieved the ability to obtain the real-time response of cells to shear stress under simulated hydrodynamic conditions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for using biochip for cell fluid experiment
  • Method for using biochip for cell fluid experiment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] In the first step, the selected chip shape specifications are as follows: the sides of culture area 13 are composed of arc walls with R=0.8mm in reverse series, and the sides of culture area 27 are made of arc walls with R=4mm. Arc-concave-convex chips formed in series. The length L of the cultivation zone 13 and the cultivation zone 27 is 25mm, the widest dimension D of the cultivation zone 13 and the cultivation zone 27 is 10mm, and the width dimension of the connecting channel is d=1mm. The experimental fluid injection flow rate was 15 μL / S and 30 μL / S. Input the shape and size of the selected rectangular culture area 3 and conical culture area 7 of the chip, as well as the dimensions of all the channels into the computer, and use CAD software to draw; then import the drawing data into the fluid simulation software, and calculate the experimental fluid flow rate. The fluid flow network of culture zone one 3 and culture zone two 7.

[0019] In the second step, use m...

Embodiment 2

[0024] In the first step, the selected chip shape specifications are as follows: the side length of the culture area-3-pointed cone is 0.5mm, and the convex-concave angle between the two sides is α 1 =30° sharp angle. The side length of the pointed cone in culture area 27 is 4mm, and the convex-concave angle between the two sides is α 2 =80° sharp angle. The length L of the cultivation zone 13 and the cultivation zone 27 is 40mm, the widest dimension D of the cultivation zone 13 and the cultivation zone 27 is 30mm, and the width dimension d of the connecting channel is 2.5mm. The experimental fluid injection flow rate was 25 μL / S and 50 μL / S. Input the shape and size of the selected rectangular culture area 3 and conical culture area 7 of the chip, as well as the dimensions of all the channels into the computer, and use CAD software to draw; then import the drawing data into the fluid simulation software, and calculate the experimental fluid flow rate. The fluid flow networ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a use method of a biological chip for a cell fluid experiment. The use method is characterized by comprising the following steps: 1, calculating a fluid flowing network of a cell culture area at an experimental fluid flowing speed according to shapes and specifications of selected chips; 2, classifying the selected chips into an experiment group and a contrast group, and culturing experimental cells under the same condition; 3, after the cells grow in an attachment manner, continuously injecting fluid which is required by the experiment and has a certain flowing speed; 4, detecting the differentiation situation of the cells under the acting force of the fluid through a high-speed high-definition camera; 5, at the end of the experiment, discharging waste liquid. The chip has the following beneficial effects that compared with a common chip, the chip is equipped with an extra culture detection unit under different mechanical experimental conditions of the fluid, and different shear forces are applied in a cell culture process, so as to realize instant and effective trace observation; real-time monitoring can be carried out within a period of time of detection, the fluid input rate can be controlled, and the detection reliability can be guaranteed; therefore, an experimental detection result can better reflect a real cell growth environment, and people can master the influence of the shear force on the cells.

Description

technical field [0001] The invention relates to a method for using a cell culture biochip dedicated for testing, and in terms of use, it is a method for using a cell culture biochip used in a fluid experiment in a cell culture experiment. Background technique [0002] There are many difficult problems in the traditional biochip technology, mainly in the fact that the traditional biochip uses isolated genes and protein molecules, which undergo a process of extraction, separation, purification, surface adhesion, molecular binding, etc. from the intracellular environment. After a series of treatments, the treated cells change, which cannot be observed and analyzed in time. Therefore, to change this situation, it is necessary to fundamentally change the design idea of ​​biochips and design a chip that can form real-time observation and comparison. In the study of cell differentiation and proliferation, cell mechanics experiments are often used to simulate in vivo fluids, and on...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
CPCC12M23/16
Inventor 廖晓玲徐文峰徐紫宸
Owner 徐州龙肽医学检验实验室有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products