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A biochip for cell fluid experiment

A technology for biochips and experiments, applied in biochemical equipment and methods, tissue cell/virus culture devices, biochemical instruments, etc., can solve the problems of lack of cell culture biochips, reduce experiment time and errors, and ensure reliability Sexuality, the effect of micro-observation

Active Publication Date: 2018-06-26
北京初美人医疗美容门诊部有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the study of cell differentiation and proliferation, cell mechanics experiments are often used to simulate in vivo fluids, and one of the effects of cell exploration on different shear forces is one of them, but there is currently a lack of cell culture biochips for shear force experiments , has achieved the ability to obtain the real-time response of cells to shear stress under simulated hydrodynamic conditions

Method used

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  • A biochip for cell fluid experiment
  • A biochip for cell fluid experiment

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Experimental program
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Effect test

Embodiment 1

[0014] The selected chip shape specifications are as follows: the side of culture area 13 is composed of arc walls with R=0.5mm in reverse series, and the side of culture area 27 is composed of arc walls with R=3mm in series. The culture area is a chip with an arc concave-convex shape. The length L of the cultivation zone 1 3 and the cultivation zone 2 7 is 20 mm, the widest dimension D of the cultivation zone 1 3 and the cultivation zone 2 7 is 15 mm, and the width dimension d of the connecting channel 4 is 5 mm.

Embodiment 2

[0016] The selected chip shape specifications are: the sides of culture area 13 are composed of circular arc walls with R=1mm in reverse series, and the sides of culture area 27 are composed of arc walls with R=5mm in reverse series. The area is a chip with a concave-convex shape of an arc. The length L of the cultivation area 1 3 and the cultivation area 2 7 is 35 mm, the widest dimension D of the cultivation area 1 3 and the cultivation area 2 7 is 20 mm, and the width dimension of the connecting channel 4 is d=1 mm.

Embodiment 3

[0018] The selected chip shape specifications are: the side length of the culture area-3 pointed cone is 0.5mm, and the convex-concave angle between the two sides is α 1 = 60° sharp angle; the side length of the pointed cone in culture area 27 is 2mm, and the convex-concave angle α between the two sides 2 =90° sharp angle, the culture area is a chip with a sharp angle concave-convex edge. The length L of the cultivation area 1 3 and the training area 2 7 is 40 mm, the widest dimension D of the training area 1 3 and the training area 2 7 is 30 mm, and the width dimension d of the connecting channel 4 is 2 mm.

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Abstract

The invention provides a biological chip for a cell fluid experiment. The biological chip is characterized in that the rectangular chip is provided with an injection holes, connecting channels, a first cultivation area and a second cultivation area, and the chip is divided into an arc concave-convex edge shape cultivation area chip and a sharp-corner concave-convex edge shape cultivation area chip according to overlooking shapes of edges of the cultivation areas. The biological chip has the advantages that in the cell fluid experiment, cells are cultivated, different flow shear stresses are applied, real-time monitoring can be achieved in detection time periods, fluid input rate is controlled, immediate, effective and micro observation is achieved, and detection reliability of the experiment is ensured.

Description

technical field [0001] The invention relates to a test-specific cell culture biochip, in terms of use, it is a cell culture biochip for fluid experiments used in cell culture experiments. Background technique [0002] There are many difficult problems in the traditional biochip technology, mainly in the fact that the traditional biochip uses isolated genes and protein molecules, which undergo a process of extraction, separation, purification, surface adhesion, molecular binding, etc. from the intracellular environment. After a series of treatments, the treated cells change, which cannot be observed and analyzed in time. Therefore, to change this situation, it is necessary to fundamentally change the design idea of ​​biochips and design a chip that can form real-time observation and comparison. In the study of cell differentiation and proliferation, cell mechanics experiments are often used to simulate in vivo fluids, and one of the effects of cell exploration on different s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
CPCC12M23/16
Inventor 廖晓玲徐文峰徐紫宸
Owner 北京初美人医疗美容门诊部有限公司
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