Using method of high-flux pattern induced cell screening chip

Abstract

The invention provides a using method of a high-flux pattern induced cell screening chip. The using method is characterized by comprising the following steps: firstly, feeding samples: dividing stem cell mixed liquid and culture liquid of the same variety and concentration into two groups, feeding the two groups of the stem cell mixed liquid and culture liquid from four injection holes, ensuring the consistency of sample feeding flow lengths, stopping feeding the samples after cells completely enter a first-stage circular culture chamber and a second-stage circular culture chamber, standing the chip into which the samples are added for 5 to 10 minutes, slowly adding equal amount of the culture liquid from the four injection holes after the cells precipitate, flushing the cells in various channels to sample outlets, and discharging excessive cells; secondly, ventilating; thirdly, washing and the like. The using method of the high-flux pattern induced cell screening chip has the beneficial effect that more channels and culture chambers can be set flexibly to enhance a cultures system by the chip according to experimental requirements. A plurality of indexes can be detected in a limited area of the chip, so that instant, effective and trace detection can be achieved. Real-time monitoring can be performed in a detecting time period so as to control nutrient substances or investigate the input dose of a medicine, and ensure the reliability of detection; therefore, a result can reflect a real cell growth environment better, and a finally required detection purpose is achieved.

Description

technical field [0001] The invention relates to a method for using a biochip for cell culture in a cell screening test, and in terms of use, it is a method for using a screening chip for a high-throughput pattern-induced cell experiment. Background technique [0002] Biochip is a high-tech that has developed rapidly in the field of biomedical engineering in the past 20 years. Because of its high-throughput, miniaturization and automation characteristics, it conforms to the principles of fast, efficient, and low-cost industrial operation, and has very strong application prospects in the fields of disease diagnosis, drug screening, and cell culture. At present, there are many experiments on the differentiation and proliferation of various types of stem cells with various patterns, but the patterns for such experiments are relatively complicated, and the requirements for the same experimental conditions in cell comparison experiments are also very strict, so it is necessary to ...

AI Technical Summary

Problems solved by technology

At present, there are many experiments on the differentiation and proliferation of various types of stem cells with various patterns, but the patterns for such experiments are relatively complicated, and the requirements for the same experimental conditions in cell comparison experiments are also very strict, so it is necessary to have an easy-to-process Equipment for such experiments

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