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Ecological cultivation special bio-fertilizer of dendrobium overgrown on tea tree and application thereof

A biological fertilizer, a technology of imitating wild cultivation, applied in the field of fertilizer biological fermentation fertilizer, can solve the problems such as the survival rate and yield instability of dendrobium seedlings and tea trees, and achieve the effects of improving the survival rate and growth quality, yield and survival rate.

Inactive Publication Date: 2017-06-13
贵州涵龙生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent (Application No.: 201410024655.2) aims at the lack of medicinal value of pure artificially planted Dendrobium, and provides a method of imitating wild cultivation of mycorrhizal Dendrobium seedling tea trees: fermentation of mycorrhizal fungi, cultivation of Dendrobium tissue culture seedlings , the symbiotic cultivation of Dendrobium seedlings and mycorrhizal fungi, the mycorrhized Dendrobium seedling hardening method, the dendrobium tissue culture seedlings and the epiphytic cultivation method of tea trees, in order to improve the medicinal value of Dendrobium and improve economic benefits, but only mention And the imitation wild cultivation method does not involve the fertilizer in the symbiotic cultivation, and the survival rate and yield in the symbiosis of Dendrobium seedlings and tea trees are not stable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The mycorrhizal fungus fermentation liquid of Dendrobium officinale is prepared by the following method: select the fresh nutrient roots of Dendrobium officinale, and use calcium hypochlorite solution to sterilize the surface, then rinse 4 times with sterile water, and then cut the roots into Place small pieces on the PAD separation medium and culture in dark at 30°C. After the mycelia grow into colonies from the root segment, punch holes at the edge of the colony to form bacterial flakes, and insert the flakes into the expansion medium at 20°C. Under shaking culture for 30 days, the mycorrhizal fungus fermentation liquid of Dendrobium officinale can be obtained; the expansion medium is: sucrose 1-30g / L, peptone 1-10g / L, yeast extract 1-10g / L potassium dihydrogen phosphate 0.1-20g / L, magnesium sulfate 0.1-20g / L, ground peat moss 1-200g / L, pH 4-9.

[0024] Sterilize the fermentation medium at 122°C for 20 minutes; the fermentation medium is: sphagnum moss and / or distill...

Embodiment 2

[0027] The mycorrhizal fungus fermentation liquid of Dendrobium officinale is prepared by the following method: select the fresh nutrient roots of Dendrobium officinale, and use calcium hypochlorite solution to sterilize the surface, then rinse 4 times with sterile water, and then cut the roots into Place small pieces on the PAD separation medium and cultivate in dark at 40°C. After the mycelia grow into colonies from the root segment, punch holes at the edge of the colony to form bacterial flakes, and insert the flakes into the expansion medium at 10°C. Under shaking culture for 20 days, the mycorrhizal fungus fermentation liquid of Dendrobium officinale can be obtained; the expansion medium is: sucrose 1-30g / L, peptone 1-10g / L, yeast extract 1-10g / L potassium dihydrogen phosphate 0.1-20g / L, magnesium sulfate 0.1-20g / L, ground peat moss 1-200g / L, pH 4-9.

[0028] Sterilize the fermentation medium at 124°C for 25 minutes; the fermentation medium is: sphagnum moss and / or disti...

Embodiment 3

[0031] The mycorrhizal fungus fermentation liquid of Dendrobium officinale is prepared by the following method: select fresh nutrient roots of Dendrobium officinale, and sterilize the surface with calcium hypochlorite solution, then rinse with sterile water for 5 times, and then cut the roots into Place small pieces on the PAD separation medium and culture in dark at 50°C. After the mycelium grows into colonies from the root segment, punch holes at the edge of the colony to form bacterial flakes, and insert the flakes into the expansion medium at 30°C. Under shaking culture for 1 day, the mycorrhizal fungus fermentation liquid of Dendrobium officinale can be obtained; the expansion medium is: sucrose 1-30g / L, peptone 1-10g / L, yeast extract 1-10g / L potassium dihydrogen phosphate 0.1-20g / L, magnesium sulfate 0.1-20g / L, ground peat moss 1-200g / L, pH 4-9.

[0032] Sterilize the fermentation medium at 125°C for 10 minutes; the fermentation medium is: sphagnum moss and / or distiller...

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Abstract

The invention relates to an ecological cultivation special bio-fertilizer of dendrobium overgrown on a tea tree, and belongs to the technical field of biological fermentation fertilizer. The bio-fertilizer is prepared from 1-50 parts of dendrobium candidum mycorrhizal fungi fermentation broth and 5-20 parts of a fermentation medium through aerobic dark incubation. According to the ecological cultivation special bio-fertilizer of the dendrobium overgrown on the tea tree, Sphagnum and / or distillers' grains is fermented with the dendrobium mycorrhizal fungi, and the fertilizer not only has the dendrobium mycorrhizal fungi, but also has necessary elements needed by the growth of dendrobium and tea (such as potassium, magnesium, manganese, ammonium, humic acid, amino acid and the like). Although the tea tree itself is good in growth, through the adoption of the bio-fertilizer, the survival rate of dendrobium overgrown on the tea tree can be remarkably increased, and the growth quality of the dendrobium overgrown on the tea tree can be remarkably improved. Experiments and studies show that, comparing the situation that the ecological cultivation special bio-fertilizer of the dendrobium overgrown on the tea tree is adopted with the situation that the bio-fertilizer is not adopted, the survival rate is increased by roughly 50%, and the yield is quintupled.

Description

technical field [0001] The invention relates to a special biological fertilizer for the ecological cultivation of dendrobium epiphytic tea trees, and belongs to the technical field of fertilizer biological fermentation fertilizers. Background technique [0002] Dendrobium is a traditional precious Chinese herbal medicine in my country, which is recorded in all previous editions of Chinese Pharmacopoeia. The original plant of the traditional Chinese medicine Dendrobium is a perennial herbaceous plant of the genus Dendrobium Sw. in the family Orchidaceae. For many years, commercial Dendrobium has mainly relied on wild medicinal Dendrobium resources. With the continuous deepening of the development and utilization of medicinal Dendrobium and the increasing demand at home and abroad, the wild medicinal Dendrobium resources in my country have been severely damaged, and some areas are even facing depletion. In recent years, people have gradually begun to practice domestic cultiva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G17/00C05G1/00
CPCA01G17/005A01G22/00C05B7/00C05G5/20C05C3/00C05D5/00C05D9/02C05F5/008C05F11/00C05F11/08
Inventor 刘焱晶
Owner 贵州涵龙生物科技有限公司
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