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Straw degradation and acidification fungicide and preparation method thereof

A technology of acidifying bacteria and straw, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problem that hemicellulose degrading enzymes affect the degradation rate of straw raw materials, the composition of strains in the hydrolysis phase is not clear, and the cellulase system Insufficient components and other problems, to achieve the effect of wide application range of pH, reduced hydraulic retention time, and complete enzyme system

Active Publication Date: 2017-06-09
河南天冠纤维乙醇有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its cellulase system has incomplete components and low activity, and the lack of hemicellulose degrading enzymes seriously affects the degradation rate of straw raw materials
Chinese invention patent CN104531767A discloses a production process for aerobic acidification and anaerobic fermentation of straw to produce biogas, using a two-phase method to produce straw biogas, but the composition of the strains in the hydrolysis phase is not clear

Method used

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  • Straw degradation and acidification fungicide and preparation method thereof
  • Straw degradation and acidification fungicide and preparation method thereof
  • Straw degradation and acidification fungicide and preparation method thereof

Examples

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Effect test

preparation example Construction

[0022] Among them, Trichoderma reesei ( Trichoderma reesei ) fermentation broth is produced by Trichoderma reesei ( Trichoderma reesei ) CGMCC No.9537 fermented liquid obtained by fermentation. Among them, Trichoderma reesei ( Trichoderma reesei ) CGMCC No.9537 was deposited in the China General Microorganism Culture Collection Management Center on August 25, 2014, and the preservation number is CGMCC No.9537. The strain has been disclosed in the patent CN104328057A. Trichoderma reesei ( Trichoderma reesei ) preparation method of fermented liquid, refer to Trichoderma reesei disclosed in Chinese patent CN104328057A ( Trichoderma reesei ) The fermentation culture method of CGMCC No.9537, specifically comprises the following steps:

[0023] ①Seed solution preparation: Trichoderma reesei ( Trichoderma reesei ) CGMCC No.9537 was inserted into PDA slant medium, cultured at 29°C for 144h, and the spores were covered with slant, washed with sterile saline to prepare 10 7 The s...

Embodiment 1

[0035] A kind of stalk degrading acidification bacterium agent, comprises Trichoderma reesei ( Trichoderma reesei ) fermentation broth, Trichoderma viride ( Trichodermaviride ) fermentation broth, Acetobacter ( Acetobacter aceti ) fermented liquid and Nanyang No. 5 yeast fermented liquid, the volume ratio of the Trichoderma reesei fermented liquid, Trichoderma virides fermented liquid, Acetobacter fermented liquid and Saccharomyces fermented liquid is 3:3:2:2.

[0036] The Trichoderma reesei ( Trichoderma reesei ) The enzyme activity of fermented liquid is 100U / ml, and described Trichoderma viride ( Trichodermaviride ) fermented liquid enzyme activity is 30000U / ml, the acetic acid bacteria ( Acetobacter aceti ) at a concentration of 2×10 6 CFU / ml, the concentration of the yeast is 1 × 10 6 CFU / ml.

[0037] The Trichoderma reesei ( Trichoderma reesei ) for Trichoderma reesei ( Trichoderma reesei ) CGMCC No.9537, the Trichoderma viride ( Trichodermaviride ) for T...

Embodiment 2

[0042] A kind of stalk degrading acidification bacterium agent, comprises Trichoderma reesei ( Trichoderma reesei ) fermentation broth, Trichoderma viride ( Trichodermaviride ) fermentation broth, Acetobacter ( Acetobacter aceti ) fermented liquid and Nanyang No. 5 yeast fermented liquid, the volume ratio of the Trichoderma reesei fermented liquid, Trichoderma virides fermented liquid, Acetobacter fermented liquid and Saccharomyces fermented liquid is 3:3:2:2.

[0043] The Trichoderma reesei ( Trichoderma reesei ) The enzyme activity of fermented liquid is 300U / ml, and described Trichoderma viride ( Trichodermaviride ) fermented liquid enzyme activity is 10000U / ml, the acetic acid bacteria ( Acetobacter aceti ) concentration is 4×10 6 CFU / ml, the concentration of the yeast is 2×10 6 CFU / ml.

[0044] Please refer to Example 1 for the types of bacteria in the straw-degrading and acidifying bacterial agent in this example and their preparation methods.

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Abstract

The invention provides a straw degradation and acidification fungicide and a preparation method thereof, and belongs to the biological technical field. The straw degradation and acidification fungicide comprises trichoderma reesei fermentation liquor, trichoderma viride fermentation liquor, acetobacter aceti fermentation liquor and saccharomycetes fermentation liquor, wherein the volume ratio of the trichoderma reesei fermentation liquor to the trichoderma viride fermentation liquor to the acetobacter aceti fermentation liquor to the saccharomycetes fermentation liquor is 3: 3: 2: 2. The fungicide makes effective use of hydrolase which is produced by mildew and can efficiently hydrolyze cellulose and hemicelluloses and is matched with yeast strains and bacillus aceticus to quickly transform straw substances into organic acid such as acetic acid for producing methane bacteria; by the synergistic effect, the gas producing rate is increased, the methane fermenting time is shortened, the equipment utilization rate is increased, and the investment cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of straw biological treatment, and in particular relates to a straw degrading acidifying bacterial agent and a preparation method thereof. Background technique [0002] In recent years, the society's demand for energy has been increasing. The excessive development and utilization of fossil energy such as coal and oil has not only brought energy problems, but also brought many environmental problems such as air pollution, which has seriously affected the sustainable development of human society. At present, clean energy and green energy have become a hot issue and development direction of common concern to all mankind. As a clean and renewable energy source, biogas has attracted more attention due to its wide source of raw materials and easy industrialization and promotion. [0003] With the energy conversion of agricultural waste resources, straw is a very rich resource. The annual output of straw in my coun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C12P5/02C12R1/885C12R1/02
CPCC12N1/20C12P5/023Y02E50/30
Inventor 张晓阳王林风赵子高闫德冉李文欢
Owner 河南天冠纤维乙醇有限公司
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