Lung cancer targeted low-toxicity quantum dot preparation

A quantum dot and targeting technology, applied in the field of biotechnology and nano-biological materials, can solve the problems of high toxicity, difficult tumor tracing and detection, etc., and achieve the effect of simple preparation process and wide applicability

Inactive Publication Date: 2017-06-09
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The current quantum dot preparations are all made by traditional preparation techniques, which are not only highly toxic but also have no ability to target tumor cells, making them difficult to use for tumor tracing and detection

Method used

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  • Lung cancer targeted low-toxicity quantum dot preparation
  • Lung cancer targeted low-toxicity quantum dot preparation
  • Lung cancer targeted low-toxicity quantum dot preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 prepares quantum dot preparation

[0034] (1) Preparation of quantum dots: the appropriate amount of CdCl 2 , NaHTe and MPA are placed in the reactor, and NaBH with pH=9.0 4 After dissolving, react continuously at 95°C for 16 hours under the protection of nitrogen, and then add a certain amount of Na to the reactor 2 S, continuously reacted at 60°C for 1.5 hours, the ratio of Cd:Te:S:MPA in the reactor was 1:0.45:0.0125:4.161, and stored at 2°C;

[0035] (2) Preparation of quantum dots targeting lung cancer: Dissolve C-RGD in PBS (pH=7.4) to make a 1 mg / mL RGD short peptide solution (dissolved at 4°C), then molar ratio 1:1 Add the prepared quantum dots and react at 4°C for 4 hours;

[0036] (3) Preparation of quantum dot preparation: Dissolve chloroquine in 0.01M PBS (pH=7.4) and make a 10 μM chloroquine solution, then dissolve the prepared quantum dots with RGD in the solution so that the heavy metal concentration Cd 2+ = 1 μM; The identification of th...

Embodiment 2

[0039] Example 2. Comparison experiment of lung cancer targeting between quantum dots prepared in the present invention and ordinary quantum dots

[0040] Lung cancer A549 cells and H1975 cells were seeded on laser confocal dishes (1×10 5 After 24 hours of incubation, the cells were allowed to adhere to the wall; after adding the quantum dots and common traditional quantum dots (20nM) and incubating for 2 hours, the cells were washed with PBS for 3 times and the situation of the cells bound to the quantum dots was observed by confocal laser, as shown in figure 2 As shown, A549 cells have no obvious fluorescence after co-incubating with traditional quantum dots for 2 hours, but there is obvious fluorescence in the cell membrane and cells after co-incubating with the quantum dot preparation targeting lung cancer for 2 hours, which proves that the quantum dots specifically target and Can bind to lung cancer cells.

Embodiment 3

[0041] Example 3. In vivo toxicity evaluation test of this quantum dot preparation

[0042] 0.2nmol of quantum dots and the prepared quantum dot preparations were injected into the mice through the tail vein respectively, and observed for 30 days respectively. No mice died in each group within 30 days. Anatomical analysis of its organs, the results are as follows image 3 As shown, after 30 days, the liver, kidney and spleen of common quantum dots were seriously damaged, and the damage caused by the prepared quantum dot preparation to the liver, kidney and spleen was significantly smaller than that caused by common quantum dots, proving that the prepared Quantum dot preparations have relatively low toxicity in vivo, and new preparations made with autophagy inhibitors can reduce the toxicity of quantum dots in vivo.

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Abstract

The invention relates to the fields of biotechnology and nano biomaterials, and relates to a preparation method and an application of a quantum dot preparation which is low-toxicity and is applicable to lung cancer diagnosis. A composition or the preparation is prepared by linking conventional quantum dots to lung cancer targeting molecules and then combining an obtained material with an autophagy regulating agent. Especially, the quantum dot preparation is composed of one or more lung cancer targeting quantum dots and one or more autophagy/enhancing agents. The quantum dot preparation prepared by the invention has the characteristics that the quantum dot preparation is lower than general quantum dot preparations in toxicity, the quantum dot preparation is capable of achieving specific targeting of lung cancer and the like. The preparation method of the preparation is simple in process, broad in application scope and applicable to large-scale production. Furthermore, the preparation is mainly used for preparing preparations for diagnosing, treating and detecting the lung cancer and the other cancers. Meanwhile, the preparation is also suitable for preparing preparations for in-vivo cancer cell tracing and localizing.

Description

technical field [0001] The invention belongs to the field of biotechnology and nano-biological materials, and relates to a low-toxicity quantum dot preparation specifically targeting lung cancer and its application, and more specifically relates to the new quantum dot targeting lung cancer and its preparation method and its combination with a One or more autophagy inhibitors are used to make new quantum dot preparations or compositions, thereby reducing the toxicity of traditional quantum dot preparations. Background technique [0002] Statistics show that lung cancer is one of the most common malignant tumors in the world, and its case fatality rate ranks first among malignant tumors. Because there is often no special clinical manifestation in the early stage, 80% of lung cancer patients are already in the middle and late stage when they are diagnosed, and they can only receive palliative treatment, resulting in a short survival time; while the 5-year survival rate of early...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K45/00A61K31/4706A61P35/00
CPCA61K49/0067A61K31/4706A61K45/00A61K49/0019A61K49/0056
Inventor 鞠佃文范佳君王绍飞李玉彬章旭耀王一辰宋平王子玉
Owner FUDAN UNIV
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