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Human papilloma virus constructs

A technology of constructs and papillomas, applied in the direction of viruses/bacteriophages, viruses, viral peptides, etc., can solve the problems of expensive vaccine production, inferior to capsicle products, and type-specific dominance

Inactive Publication Date: 2017-05-31
UNIV OF COLORADO THE REGENTS OF
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these types of vaccines are expensive to produce, require eukaryotic expression systems or complex purification, and are less stable than capsomer preparations
VLP vaccines do not confer cross-protection against other papillomavirus serotypes because neutralizing immune responses tend to be type-specific

Method used

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  • Human papilloma virus constructs
  • Human papilloma virus constructs
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Examples

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Embodiment

[0059] Certain embodiments presented herein are illustrated by presenting the following examples. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered to function well in the practice disclosed herein. However, those of skill in the art will appreciate, in light of the disclosure herein, that many changes can be made in the certain embodiments disclosed and still obtain a like or similar result without departing from the spirit and scope of the present disclosure. .

[0060] In some of the exemplary methods below, the coding region of the truncated HPV L1 protein was amplified by PCR from an E. coli codon-optimized gene and cloned downstream of the pTac promoter, thereby producing Untagged peptides. Alternatively, the native HPV L1 gene can be used in place of the codon-optimized version. Also, the full-length L1 coding region or other truncated gene versions can be used for gene con...

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Abstract

Embodiments of the present invention provide compositions and methods for recombinantly generating tagless constructs of proteins or peptides. In certain embodiments, recombinant proteins or peptides disclosed herein concern human papilloma virus (HPV). Other embodiments concern using these constructs in compositions to elicit immune responses in a subject to one or more HPV types. Therapeutic and prophylactic vaccines for the prevention and treatment of viral infections are also disclosed. Nucleic acids and expression vectors coding for constructs contemplated herein are provided. In certain embodiments, an HPV capsid protein generated is devoid of any fusion tags. In addition, truncated forms of HPV L1 are contemplated.

Description

[0001] Government funding [0002] This invention was made with government support under Grant No. CA098252 awarded by the National Institutes of Health. The government has certain rights in this invention. [0003] priority [0004] This PCT application claims priority to US Provisional Application No. 62 / 014,495, filed June 19, 2014. This application is hereby incorporated by reference in its entirety for all purposes. [0005] field of invention [0006] Embodiments of the present invention provide novel vaccines and diagnostic reagents for the prevention, treatment and / or diagnosis of human papillomavirus infection and cervical cancer associated therewith. Some embodiments herein relate to human papillomavirus (HPV) constructs without tags, trackers or fusion elements. These preparations can be used to induce an immune response to a target HPV in a subject. In certain embodiments, the HPV constructs produced by the compositions and methods disclosed herein are capsomer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/37A61K39/12A61P31/20
CPCA61K39/12A61K2039/53A61P31/20C07K14/005C12N2710/20022C12N2710/20034C12N2800/101C12N2800/22C12N7/00C12N15/70
Inventor R·L·加西亚D·G·梅思雅克
Owner UNIV OF COLORADO THE REGENTS OF
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