Luciferase co-immunoprecipitation kit for detecting mammalian lyme diseases

Abstract

The invention discloses a luciferase co-immunoprecipitation kit for detecting a mammalian lyme disease. The luciferase co-immunoprecipitation kit comprises the following components: (1) a sample diluent; (2) a fusion protein solution composed of ranilla luciferase and a borrelia burgdorferi surface membrane protein OspC antigen; (3) a proteinA / G-coated ELISA plate; (4) washing liquid; (5) a luciferase substrate. The luciferase co-immunoprecipitation kit disclosed by the invention adopts a luciferase co-immunoprecipitation method and can be used for detecting the lyme disease and the onset period thereof according to the fluorescence intensity. The method is quick, simple and convenient, high in sensitivity, high in signal-noise ratio, and stable and reliable in measured C:\Program Files\Youdao\Dict\7.1.0.0421\resultui\dict\value.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a luciferase immunoprecipitation kit for detecting Lyme disease in mammals. Background technique [0002] Lyme disease is a spirochete infectious disease mediated by ticks, which is a natural foci disease caused by Borrelia burgdorferi. In 1985, my country first discovered the disease case in the forest area of ​​Heilongjiang Province, and the main clinical manifestation of the disease was nervous system damage. The most common nervous system damages are meningitis, encephalitis, cranial neuritis, motor and sensory neuritis. In the first stage of Lyme disease, only antibiotics can be effective, and in the second and third stages, it is useless to use antibiotics, especially for nervous system damage, and there is no specific treatment. In the early stage, it is characterized by chronic migratory erythema of the skin, and later nerve, heart or joint lesions appear. ...

AI Technical Summary

Problems solved by technology

IFA is greatly affected by experiments, and there are many human interference factors, which can easily cause false positives or false negatives

[0009] 4. Enzyme-linked immunoassay (ELISA): ELISA is mainly used to detect Lyme disease-specific antibody IgG, which requires high enzyme activity and sensitivity, and its specificity depends on the quality of antigen preparation; and its combination The preparation of the compound has not been standardized, which makes the experimental results not very reproducible and requires multiple tests

In addition, if the concentration of antigen and enzyme conjugate is low, the final absorbance value is too small, which is prone to false negatives

[0010] 5. Western blotting: high specificity and certain application value, but the operation is more complicated, and it is not easy for general laboratories

[0011] 6. Polymerase chain reaction (PCR): It is a sensitive and specific method, but due to the short and irregular time of bacteremia in patients, it has its fundamental limitations in clinical laboratory diagnosis. It is different from PCR in the diagnosis of other hospital microorganisms. It is also obviously misleading, and it is still in the stage of laboratory research