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Method for detecting quinolone medicine in milk or milk product by adopting CPE (Cloud Point Extraction)-LC (Liquid Chromatography)/MS (Mass Spectrometry)/MS method

A quinolone and liquid chromatography technology, which is applied to measurement devices, instruments, scientific instruments and other directions, can solve problems such as environmental pollution, limit wide application, etc., and achieve the effects of saving analysis time, less dosage, and simplifying pretreatment steps.

Inactive Publication Date: 2017-05-31
INSPECTION & QUARANTINE TECH CENT OF YANTAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Problems solved by technology

Due to the extensive use of organic solvents, it pollutes the environment and limits its wide application.

Method used

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  • Method for detecting quinolone medicine in milk or milk product by adopting CPE (Cloud Point Extraction)-LC (Liquid Chromatography)/MS (Mass Spectrometry)/MS method
  • Method for detecting quinolone medicine in milk or milk product by adopting CPE (Cloud Point Extraction)-LC (Liquid Chromatography)/MS (Mass Spectrometry)/MS method
  • Method for detecting quinolone medicine in milk or milk product by adopting CPE (Cloud Point Extraction)-LC (Liquid Chromatography)/MS (Mass Spectrometry)/MS method

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Embodiment Construction

[0054] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0055] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0056] 1. Establishment of detection method

[0057] 1. Experimental materials

[0058] 1.1 Raw material of the sample: the milk is the pure milk of Yili Group.

[0059] 1.2 Veterinary drug standard products: enrofloxacin, ciprofloxacin, norfloxacin, ofloxacin, nalidixic acid, sarafloxacin, lomefloxacin, pefloxacin, oxolinic acid, orbifloxacin, Flumequine was purchased from Dr. Ehrenstorfer, Germany (purity>99.0%).

[0060] 1.3 Reagents: PEG 4000, Triton X-100 (Shanghai Lingfeng Chemical Reagent Company, chemically pure); Tween 20, Tween 40 (Tianjin Bodi Chemical Co., Ltd., analytically pure); Triton X-114 (Shanghai Hushang Biotechnology Co., Ltd. Co., Ltd., analytically pure); acetonitrile (Tianjin Bodi Chemical Co., Ltd., ...

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Abstract

The invention discloses a method for detecting quinolone medicine in milk or a milk product by adopting a CPE (Cloud Point Extraction)-LC (Liquid Chromatography)-MS (Mass Spectrometry) / MS method. The method comprises the following steps: (1) demulsifying the milk or the milk product, and taking supernatant A after centrifuging; (2) carrying out CPE on the supernatant A, and taking supernatant B after centrifuging an obtained enriched phase; (3) detecting the supernatant B by adopting an LC-MS / MS method, thus realizing the detection on the quinolone medicine in the milk or the milk product. According to the method disclosed by the invention, a CPE method is adopted, and a CPE-LC-MS / MS determination method of a target compound is established through a fortified recovery test on the quinolone medicine. The technology not only is capable of greatly simplifying pretreatment steps of a sample and saving the analysis time, but also is capable of reducing the pollution to an environment due to a small dosage of an organic solvent, the technology is an environment-friendly sample pre-treatment technology, and the loss brought by decreaming and emulsifying of the sample is avoided.

Description

technical field [0001] The invention relates to a method for detecting quinolones in milk or milk products by applying cloud point extraction-liquid chromatography tandem mass spectrometry, and belongs to the technical field of food detection methods. Background technique [0002] Veterinary drugs refer to chemical substances used in food animals for the purpose of treatment, prevention or diagnosis, or to change neurological function or behavior. The widespread use of veterinary drugs plays a huge role in ensuring the steady development of animal husbandry, increasing the supply of animal husbandry products, reducing economic losses caused by animal diseases, and controlling the spread of zoonotic diseases between animals and humans. With the modernization, intensification and large-scale development of animal husbandry production, the use of veterinary drugs will inevitably increase, but the residues of various drugs will inevitably appear in meat, eggs, and milk. After t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 曹鹏李晓玉卢志晓尹大路梁君妮鲁闽贺丽娜胡巧茹
Owner INSPECTION & QUARANTINE TECH CENT OF YANTAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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