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Method for detecting β-receptor agonists in milk or milk products by cloud point extraction-liquid chromatography tandem mass spectrometry

A receptor agonist and dairy product technology, which is applied in the field of β-receptor agonist drugs and food testing, can solve problems such as environmental pollution and restrictions on wide application, and achieve analysis time saving, less dosage, and simplified pretreatment The effect of the steps

Active Publication Date: 2019-07-16
INSPECTION & QUARANTINE TECH CENT OF YANTAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the extensive use of organic solvents, it pollutes the environment and limits its wide application.

Method used

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  • Method for detecting β-receptor agonists in milk or milk products by cloud point extraction-liquid chromatography tandem mass spectrometry
  • Method for detecting β-receptor agonists in milk or milk products by cloud point extraction-liquid chromatography tandem mass spectrometry
  • Method for detecting β-receptor agonists in milk or milk products by cloud point extraction-liquid chromatography tandem mass spectrometry

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0051] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0052] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0053] 1. Materials and methods

[0054] 1. Experimental materials

[0055] 1.1 Raw material of the sample: the milk is the pure milk of Yili Group.

[0056] 1.2 Beta-receptor agonist veterinary drug standard standard Clenbuterol, Ractopamine, Salbutamol, Terbutaline, Mapenterol, Mab Mabuterol, Penbutolol, Tulobuterol, Clorprenaline, Cimaterol, Cimbuterol, Zipaterol (Zilpaterol), Propranolol, isotope internal standard albuterol-d3 (salbuterol-d3), clenbuterol-d9 (clenbuterol-d9), lycadopamine-d3 (ractopamine-d3) were all purchased from Dr. .Ehrenstorfer, purity >95%,

[0057] 1.3 Reagents: PEG 2000, PEG 4000, PEG 6000, PEG 8000 (Shanghai Lingfeng Chemical Reagent Company, chemically pure); acetonitrile (Tianjin Bodi Chemica...

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Abstract

The invention discloses a method for detecting beta-receptor stimulant medicine in milk or a milk product by adopting a CPE (Cloud Point Extraction)-LC (Liquid Chromatography)-MS (Mass Spectrometry) / MS method. The method comprises the following steps: (1) demulsifying the milk or the milk product, and taking supernatant A after centrifuging; (2) carrying out CPE on the supernatant A, and taking supernatant B after centrifuging an obtained enriched phase; (3) detecting the supernatant B by adopting an LC-MS / MS method, thus realizing the detection on the beta-receptor stimulant medicine in the milk or the milk product. According to the method disclosed by the invention, a CPE method is adopted, and a CPE-LC-MS / MS determination method of a target compound is established through a fortified recovery test on the beta-receptor stimulant medicine. The method not only is capable of greatly simplifying pretreatment steps of a sample and saving the analysis time, but also is capable of reducing the pollution to an environment due to a small dosage of an organic solvent, the method is an environment-friendly sample pre-treatment technology, and the loss brought by decreaming and emulsifying of the sample is avoided.

Description

technical field [0001] The invention relates to a method for detecting beta-receptor agonist drugs in milk or milk products by applying cloud point extraction-liquid chromatography tandem mass spectrometry, and belongs to the technical field of food detection methods. Background technique [0002] Veterinary drugs refer to chemical substances used in food animals for the purpose of treatment, prevention or diagnosis, or to modify neurological function or behavior. The widespread use of veterinary drugs plays a huge role in ensuring the steady development of animal husbandry, increasing the supply of animal husbandry products, reducing economic losses caused by animal diseases, and controlling the spread of zoonotic diseases between animals and humans. With the modernization, intensification and large-scale development of animal husbandry production, the use of veterinary drugs will inevitably increase, but the residues of various drugs will inevitably appear in meat, eggs, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 曹鹏梁君妮胡巧茹李晓玉贺丽娜鲁闽尹大路
Owner INSPECTION & QUARANTINE TECH CENT OF YANTAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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