Method for rapidly identifying holothuria fuscopunctata and holothuria scabra

Abstract

The invention discloses a DNA (Deoxyribonucleic Acid) barcode molecular identification method of two types of sea cucumbers (holothuria fuscopunctata and holothuria scabra) and a CO I sequence of the sea cucumbers. The method is used for carrying out PCR (Polymerase Chain Reaction) amplification to obtain a CO I gene of the sea cucumbers; a PCR product is identified through agarose gel and then is sequenced; a sequencing result is compared with a public sequence; if the homology between the sequencing result and a gene sequence SEQ ID NO.1 is 99 percent or above, the method can judge that a tissue to be detected is a source of the holothuria fuscopunctata; if the homology between the sequencing result and a gene sequence SEQ ID NO.2 is 99 percent or above, the method can judge that the tissue to be detected is the holothuria scabra. According to the DNA barcode molecular identification method, a reliable DNA technology is adopted, and holothuria fuscopunctata and holothuria scabra varieties are rapidly and accurately distinguished; the accuracy and reliability are enhanced, and the safety of taking the sea cucumbers as a medicinal material is ensured.

Description

technical field [0001] The invention relates to the field of species identification, in particular to a DNA barcode molecular identification method for two kinds of sea cucumbers (ivory sea cucumber and rough sea cucumber). Background technique [0002] Sea cucumber is the general term for animals of the class Holothrioider in the phylum Echinodermata. It is a traditional sea-like food and drug resource. . The prices of different species of sea cucumbers vary greatly, but many economic species of sea cucumbers lack clear species identification during the trade process. Ivory ginseng (Holothuria fuscopunctata) is usually 6-30cm long and 0.8-10cm wide. The whole surface is grayish white or yellowish white, wrinkled or horizontally striated, and some horizontally wrinkled deeply into grooves, and the grooves are yellow or brown. . The color is darker in the middle of the back, lighter on both sides, and gradually turns white on the ventral surface; there is often an obvious ...

AI Technical Summary

Problems solved by technology

At present, the traditional morphological identification method is very susceptible to the influence of many factors such as the environment and genetics during the growth of the species itself; and the morphology is severely damaged or the characteristics disappear during the processing process.

Therefore, it is very difficult to identify sea cucumber species using traditional morphological methods, and it is urgent to establish other effective methods to quickly identify sea cucumber species in the market

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