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Preparation method and preparation system of multi-cell structure on basis of reaction-diffusion model

A diffusion-modeled, multicellular technology for enabling technologies in biomanufacturing and tissue engineering, especially in the field of bone and cartilage tissue repair

Active Publication Date: 2017-05-31
HOHAI UNIV CHANGZHOU
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method to solve the technical problems of the preparation of three-dimensional multicellular structures and the regulation of structure morphology

Method used

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  • Preparation method and preparation system of multi-cell structure on basis of reaction-diffusion model
  • Preparation method and preparation system of multi-cell structure on basis of reaction-diffusion model
  • Preparation method and preparation system of multi-cell structure on basis of reaction-diffusion model

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Embodiment 1

[0088] In this embodiment, the extracellular matrix used for three-dimensional cell culture is modified hyaluronic acid hydrogel. By adjusting the concentration of macromolecules or connecting peptide chains in the hydrogel, three-dimensional gels with different mechanical properties and different diffusion rates of protein molecules can be constructed. In this example, mesenchymal stem cells 03 in a preliminary calcified state were used because they have stronger aggregation and structure forming capabilities, and they can differentiate into cartilage and osteoblasts. The density of cells in the gel is uniform, and various multicellular structures are basically evenly distributed in the three-dimensional gel body. By uniformly adding the cytokine solution to the culture medium, various local positions of the whole three-dimensional multicellular structure can be changed in three-dimensional space.

[0089] Such as figure 1 As shown, this embodiment 1 provides a method for p...

Embodiment 2

[0127] On the basis of Example 1, this Example 2 provides a three-dimensional multicellular structure preparation system, including:

[0128] The preliminary culture unit is used to culture stem cells in vitro through the constructed three-dimensional gel system to form a series of multicellular structures with different geometric structural characteristics; and the morphology adjustment unit is used to regulate the shape of multicellular structures.

[0129] The shape adjustment unit is suitable for constructing a reaction-diffusion model, and is guided by the reaction-diffusion model to regulate the shape of the multicellular structure in the three-dimensional gel to form the desired multicellular structure.

[0130] The mathematical expression of the reaction-diffusion model is as described in Example 1.

[0131] In the present invention, under the guidance of the reaction-diffusion model, the process example of the method for preparing a three-dimensional multicellular str...

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Abstract

The invention relates to a preparation method and a preparation system of a multi-cell structure on the basis of a reaction-diffusion model. The preparation method comprises the following steps of S1, performing culture in vitro on stem cells through a built three-dimensional gel system; S2, regulating and controlling the multi-cell structure form. The method for regulating and controlling the multi-cell structure form formed by mesenchymal stem cell self tissues provided by the invention completely depend on self assembly of the cells; the necessary external structure material and the complicated processing and manufacturing process in the existing tissue in vitro building method can be completely omitted; by the whole preparation method, the mutual communication capability among a great number of cells can be more sufficiently utilized; the process is more similar to the natural formation process of the tissue in the body; a high-reliability method which is more similar to the natural tissues in the body is provided for the artificial tissue preparation.

Description

technical field [0001] The present invention is a method for regulating the growth process of multicellular structures and tissues in vitro by adding appropriate cytokines under the guidance of a mathematical model of diffusion reaction, and relates to the enabling technology of biomanufacturing and tissue engineering, especially the repair of bone tissue and cartilage tissue technical field. Background technique [0002] To achieve the repair and regeneration of biological tissue is the main goal of tissue engineering. In order to achieve the functional regeneration of tissues and even organs, more and more researchers design and manufacture various artificial substitute structures and widely apply them in biological tissue engineering, and such manufacturing is gradually developing in a more complex and refined direction. Biological tissues with various geometrical structural characteristics (such as trachea, blood vessels, lymphatic vessels, and intestines, etc.) widely ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775G06F17/50
CPCC12N5/0662C12N2501/155C12N2501/20C12N2513/00G06F30/20
Inventor 朱晓璐
Owner HOHAI UNIV CHANGZHOU
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