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Broadspectrum efficient Klebsiella pneumoniae KL1 for decoloring azo dyes and application of broadspectrum efficient Klebsiella pneumoniae KL1

A Klebsiella and azo decolorization technology, which is applied in the direction of bacteria, water pollutants, and microbial-based methods, can solve the problem of decolorization of sewage without azo dyes, the long time required for decolorization of azo dyes, and the treatment Harsh environmental requirements and other issues have achieved the effect of good market prospects, wide cultivation and adaptation conditions, and low cost

Active Publication Date: 2017-05-31
上海泽耀环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some of the strains they screened required complex culture media, some took too long to decolorize azo dyes, and some had more stringent requirements for the processing environment
In addition, the current patents on Klebsiella pneumoniae mainly involve the treatment of termite control, industrial organic phenolic sewage and oil pollution, and there is no decolorization treatment of azo dye sewage.

Method used

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  • Broadspectrum efficient Klebsiella pneumoniae KL1 for decoloring azo dyes and application of broadspectrum efficient Klebsiella pneumoniae KL1
  • Broadspectrum efficient Klebsiella pneumoniae KL1 for decoloring azo dyes and application of broadspectrum efficient Klebsiella pneumoniae KL1
  • Broadspectrum efficient Klebsiella pneumoniae KL1 for decoloring azo dyes and application of broadspectrum efficient Klebsiella pneumoniae KL1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Colony observation of Klebsiella pneumoniae strain KL1.

[0030] Take 10uL of the bacterial solution of Klebsiella pneumoniae strain KL1 in the logarithmic phase of growth, and dilute it with sterile water for 10 6 Finally, take 100uL of the diluted liquid and add it dropwise to the surface of the plate culture medium, spread it evenly with a sterilized applicator, under the conditions of 37°C and pH 5.6, let it stand at constant temperature for 1 to 3 days, and then observe the growth of the colony. Such as figure 1 shown.

[0031] Components of the above plate medium: potassium dihydrogen phosphate 2.66g / L, ammonium chloride 0.2g / L, anhydrous sodium sulfate 0.5g / L, yeast extract 3g / L, agar powder 15g / L, distilled water 1L.

[0032] The biological characteristics of Klebsiella pneumoniae strain KL1 are round colonies with rounded edges, smooth and slightly raised surface, relatively viscous, and milky white. Gram-negative bacteria were identified by Gram staining, ...

Embodiment 2

[0035] Enrichment culture of Klebsiella pneumoniae strain KL1.

[0036] Take the Klebsiella pneumoniae strain (Klebsiella pneumoniae) KL1 with an inoculation loop and transfer it to an Erlenmeyer flask (250 mL) containing 100 mL of liquid medium. Klebsiella strain KL1.

[0037] The above medium components are: Potassium dihydrogen phosphate 2.66g / L, ammonium chloride 0.2g / L, anhydrous sodium sulfate 0.5g / L, yeast extract 3g / L, distilled water 1L.

[0038] The above-mentioned culture medium, Erlenmeyer flask and other utensils were all sterilized at 121°C and 100kPa for 20 minutes.

Embodiment 3

[0040] Growth curve assay of Klebsiella pneumoniae strain KL1.

[0041]The bacterium solution of the Klebsiella pneumoniae strain KL1 (obtained by the conditional culture in Example 2) that has been cultured statically at a constant temperature for 24h is inoculated in the freshly sterilized medium with 1% (V / V), at regular intervals Take a small amount of bacterial liquid and measure its OD600 optical density value on a UV-visible spectrophotometer as a representative value of the bacterial content. After measuring for 100 hours, draw a growth curve according to the measurement time and the corresponding OD600 value. The results are as follows: image 3 shown. The growth characteristics of Klebsiella pneumoniae strain KL1 can be observed from the figure. KL1 is in the lag phase at 0-2 hours, in the logarithmic phase at 2-30 hours, and in the stable phase at 30-100 hours. The Klebsiella pneumoniae strain KL1 provided by the present invention has a shorter lag period, which is...

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Abstract

The invention relates to broadspectrum efficient Klebsiella pneumoniae KL1 for decoloring azo dyes and application of the broadspectrum efficient Klebsiella pneumoniae KL1. The 16S rRNA sequence of the KL1 is shown in SEQ ID NO: 1. The KL1 provided by the invention can be used for efficiently decoloring a variety of azo dyes, the 24h decoloring ratio reaches 90% or more, the nutritional requirements are simple, the adaptable culture conditions are relatively wide, the culture temperature is 30 DEG C to 40 DEG C, and a decoloring action can be efficiently exerted in a pH range of 4.0 to 9.0, so that the KL1 can be applied to the treatment of azo printing and dyeing wastewater and contaminated soil and has the advantages of low cost, convenience in use, good removal effect, and the like, thereby being good in market prospect.

Description

technical field [0001] The invention belongs to the field of printing and dyeing wastewater treatment, and in particular relates to a Klebsiella pneumoniae strain KL1 capable of efficiently decolorizing azo dyes and an application thereof. Background technique [0002] Dyes are widely used in industries such as textiles, leather, plastics, cosmetics and food. There are 7×105t synthetic dyes produced every year in the world, and 5% to 10% are discharged with printing and dyeing wastewater. Azo dyes are the most widely used in the textile industry, accounting for about 70%. More than 3000 kinds of azo dyes are used in various industries every year. There are one or more azo bonds (—N==N—) and sulfonic acid groups in azo dyes, which make the dye molecules toxic, high temperature resistant, very stable under acidic and alkaline conditions, and difficult to degrade naturally. are thus considered persistent organic pollutants. Dyestuffs entering natural water bodies not only d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/22C02F101/38
CPCC02F3/34C02F2101/308C02F2101/38C12N1/20Y02W10/37
Inventor 谢学辉俞承志郑秀林张庆云刘娜柳建设
Owner 上海泽耀环保科技有限公司
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