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Dictyophora rubrovolvata yzs020 strain suitable for substitute cultivation

A technology of substituting material cultivation and Dictyophora edulis, which is applied in the field of Dictyophora yzs020 strain, can solve the problems of slow mycelial growth, disordered strains, large quality fluctuations, etc., and achieve high mycelial polysaccharide content and neat fruiting Uniform, cold-resistant effect

Active Publication Date: 2017-05-31
GUIZHOU CROP VARIETIES RESOURCE INST
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] D. rubrovolvata mycelium grows slowly, has poor genetic stability, and is easy to degenerate, making it difficult to subculture
And currently there is no stable mother species, original species supply organization, nor a stable strain
Therefore, in the production process of strains, a large number of buds must be collected every year to isolate strains. Due to genetic differences or variations among strains, the strains produced and circulated in the market are confused and have poor stability, resulting in low yield of Dictyophora edulis. Stable, large fluctuations in quality
For a long time, because the red-tuo bamboo fungus planted in Guizhou is better than the long-skirted bamboo fungus planted in Fujian, it has a clear fragrance. "Fragrant-scented bamboo fungus" has become synonymous with Guizhou bamboo fungus, but so far there is no high-quality, high-yielding and stable strain

Method used

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  • Dictyophora rubrovolvata yzs020 strain suitable for substitute cultivation
  • Dictyophora rubrovolvata yzs020 strain suitable for substitute cultivation
  • Dictyophora rubrovolvata yzs020 strain suitable for substitute cultivation

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1 of the present invention: the starting strains in the following examples are all wild strains with different habitats or cultivated strains with large differences in growth. There are 40 strains in total, 5 of which are cultivated strains, and the rest are wild strains, as shown in Table 1.

[0026] Table 1 The tested Dictyophora strains and sources

[0027]

[0028] Embodiment 1 of the present invention: natural breeding of Dictyophora yzs020 strain

[0029] The natural breeding process of Dictyophora edulis strain yzs020 of the present invention comprises the following steps:

[0030] 1) Selection of natural strains: 40 strains were selected, of which 5 were cultivated types and 35 were wild types. All these strains were preserved by the Fungi Department of Guizhou Crop Variety Resources Research Institute.

[0031] 2) Preliminary screening of breeding characteristics of mother species

[0032] Through the analysis of the growth and growth speed of ...

Embodiment 2

[0055] Example 2 of the present invention: Morphological characteristics and biological characteristics of Dictyophora yzs020 strain

[0056] Morphological characteristics:

[0057] The hyphae are white and dense, the aerial hyphae are developed, multi-branched, separated, double-nucleated, and have lock-like joints. After the mycelia are stimulated by strong light or mechanical stimulation, and when the stamina of the medium is insufficient, red discoloration will occur. This is red. The unique properties of Dictyophora ( image 3 ).

[0058] The fruiting body belongs to abdominal fungi, and has obvious receptacle, stipe, fungus skirt, cap and spores after shell breaking. Among them, the receptacle is milky white, with dot-like scales on the surface, and appears red or even purple after being stimulated by strong light or machinery; the stipe is white, cylindrical, 13-18 cm long, 3.5-5 cm in diameter, spongy, hollow; the fungus skirt White, brittle, drooping from the top o...

Embodiment 3

[0062] Embodiment 3 of the present invention: Physicochemical properties of the yzs020 strain of Dictyophora edulis

[0063] Analysis of polysaccharide content:

[0064] The polysaccharide content of strain yzs020 is 1.23%, which is higher than that of cultivated strains. Please refer to Figure 4 .

[0065] Extracellular Enzyme Activity Analysis:

[0066] The filter paper enzyme activity was 5.63U / g, the CMC enzyme activity was 15.18U / g, the hemicellulase activity was 6.34U / g, and the laccase activity was 3.59U / g. The content of filter paper enzyme and CMC enzyme is relatively high, and the content of hemicellulase and laccase is relatively low, which can well decompose the cellulose in the matrix material, and is suitable for the cultivation of straw substitute material. Please refer to the specific differences Figure 5 .

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Abstract

The invention discloses a dictyophora rubrovolvata yzs020 strain suitable for substitute cultivation. The dictyophora rubrovolvata yzs020 strain is very suitable for substitute cultivation, has the advantages of rapid growth, high hypha polysaccharide content, comparatively high paper enzyme content, comparatively high CMC enzyme content, comparatively low hemicellulase content and comparatively low laccase content, can very well decompose cellulose in a matrix material and is suitable for substitute cultivation of straws. The dictyophora rubrovolvata yzs020 strain suitable for the substitute cultivation not only can realize fermentation material culture of straws of dictyophora rubrovolvata but also has the advantages of short fruiting period, strong cold resistance and so on and can also produce mushrooms in autumns; and the yield is increased by more than 10% when being compared with the yield of a control group. The mushrooms are produced in an even and neat manner and can be produced in the whole year; and the dictyophora rubrovolvata yzs020 strain suitable for the substitute cultivation has the advantages of high yield per unit, easily fallen spores and convenient processing and is suitable for large-area promotion and cultivation.

Description

technical field [0001] The invention relates to the technical field of edible fungi, in particular to a yzs020 strain of Dictyophora edulis which is suitable for substituting material cultivation. Background technique [0002] Dictyophora rubrovolvata M.Zang et al., belonging to Eumycota, Basidiomycotina, Gasteromycetes, Phallales, Phallaceae , Dictyophora (Dictyophora) fungi. The fungus has unique flavor, rich nutrition and high value. It is a good product among edible fungi, and has the reputation of "Queen of Mushrooms" and "Flower of Fungi". Wild Dictyophora edulis is mainly distributed on humus soil under bamboo forests in Guizhou, Yunnan, Sichuan and Zhejiang, and is mainly produced in various parts of Guizhou Province and Yuxi, Yunnan. The polysaccharides in the fruiting body of Dictyophora edulis are mainly composed of galactose, glucose, mannose and xylose, which have strong reducing ability and ability to inhibit hydroxyl radicals, and have obvious antioxidant ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/145C12R2001/645
Inventor 朱国胜桂阳龚光禄卢颖颖杨通静黄万兵陈娅娅张丽娜王沁胡腾文
Owner GUIZHOU CROP VARIETIES RESOURCE INST
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