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Method for culturing soil aggregate microorganisms in situ

A soil aggregate and in-situ culture technology, which is applied in the field of in-situ culture of soil aggregate microorganisms, can solve the problems of little research on the distribution characteristics of microbial biomass, and achieve the effects of stable microbial properties of soil aggregates, simple operation, and low cost

Inactive Publication Date: 2017-05-31
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there are many studies on soil aggregates and soil microbial biomass carbon and nitrogen, there are few studies on the distribution characteristics of microbial biomass in soil aggregates.

Method used

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  • Method for culturing soil aggregate microorganisms in situ
  • Method for culturing soil aggregate microorganisms in situ
  • Method for culturing soil aggregate microorganisms in situ

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] In the field or in the field, use a cube aluminum fresh-keeping box to collect undisturbed soil samples from top to bottom according to the soil profile, prevent crushing during transportation, and maintain the original structure of the soil; after natural air-drying at room temperature, break it into small soils of about 1cm along the natural cracks piece. When the soil moisture content reaches 8% to 10%, the dry sieve method is used to separate the aggregates of the soil samples according to the grades of >2mm, 0.25~2mm, 0.053~0.25mm, and <0.053mm; the dry sieve method will be used for The graded soil aggregates were spread on the slabs respectively to increase the contact area of ​​distilled water and improve the speed and effect of infiltration. Spray with a watering can, the water volume was 50% of the field water holding capacity, and infiltrated with distilled water for 5 minutes. The agglomerates soaked in distilled water are put into a cloth bag with a pore siz...

Embodiment 2

[0041]In the field or in the field, use a cube aluminum fresh-keeping box to collect undisturbed soil samples from top to bottom according to the soil profile, prevent crushing during transportation, and maintain the original structure of the soil; after natural air-drying at room temperature, break it into small soils of about 1cm along the natural cracks piece. When the soil moisture content reaches 10%, use the wet sieving method to place the air-dried soil sample on the uppermost layer of a set of sieves with apertures of 2mm, 0.25mm and 0.053mm, and adjust the water level of the bucket below the set of sieves to the uppermost sieve The soil sample is just submerged, and then infiltrated with distilled water at room temperature for 5 minutes. The separation of aggregates is carried out by moving the sieve up and down 3 cm, and repeating 50 times within 2 minutes to obtain > 2 mm, 0.25 ~ 2 mm, 0.053 ~ 0.25 mm And <0.053mm four particle size components, which are macroaggreg...

Embodiment 3

[0043] When the soil water content reaches 50%, use the wet sieving method to place the air-dried soil sample on the uppermost layer of a set of sieves with apertures of 2mm, 0.25mm and 0.053mm, and adjust the water level of the bucket under the set of sieves to the uppermost sieve The soil sample is just submerged, and then infiltrated with distilled water at room temperature for 5 minutes. The separation of aggregates is carried out by moving the sieve up and down 3 cm, and repeating 50 times within 2 minutes to obtain > 2 mm, 0.25 ~ 2 mm, 0.053 ~ 0.25 mm And <0.053mm four particle size components, which are macroaggregates, intermediate agglomerates, microaggregates and powder-sticky agglomerates. The agglomerates soaked in distilled water are put into a cloth bag with a pore size of 300 meshes according to the size of the particles, and the bag containing the agglomerates is placed in a dry ice incubator for low temperature preservation. Bury it into the soil according to ...

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Abstract

The invention relates to a method for culturing soil aggregate microorganisms in situ. The method comprises the following steps: guaranteeing sufficient samples; collecting 500g of each sample soil or more; carrying out particle classification on aggregates by adopting a wet screening method or a drying screening method; wetting for 5min by adopting distilled water, wherein the water adding amount is 50 percent of a field water capacity; after wetting by adopting the distilled water, transferring the aggregates into cloth bags with gaps according to grades; burying the cloth bags into the soil according to a soil section sequence; carrying out in-situ culture for 7d or more and getting close to in-situ soil microorganisms; after carrying out the in-situ culture, sampling and rapidly storing into a heat-preservation box filled with dry ice, so as to keep the temperature below 4 DEG C; bringing the heat-preservation box back into a room and carrying out -80 DEG C ultralow-temperature preservation; then determining microorganism related indexes. The method provided by the invention is simple and low in cost; the aggregate microorganisms are cultured by the in-situ soil and attribute characteristics of the in-situ microorganisms can be more directly and accurately reflected.

Description

technical field [0001] The invention mainly relates to the fields of soil biochemistry, environmental science and the like, and particularly relates to a method for cultivating soil aggregate microorganisms in situ. Background technique [0002] The soil organic carbon pool is the largest carbon pool in terrestrial ecosystems. The content of soil organic carbon is about three times that of the atmospheric carbon pool and 2.5 times that of terrestrial biological carbon. Small changes in soil organic carbon pools will have a major impact on the global carbon balance. Microbial biomass carbon is the most active and variable part of soil active organic matter, although it only accounts for 0.3%-9.9% of soil organic carbon, but It is the driving force of soil organic matter and soil nutrient transformation and cycle. It participates in various biochemical processes such as the decomposition and formation of organic matter, nutrient transformation and cycle, and is considered to b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
CPCC12N1/00
Inventor 邓蕾上官周平
Owner NORTHWEST A & F UNIV
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