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Method for extracting albumin and globulin from bovine serum and method for utilizing ox blood

A technology for albumin and globulin, applied in the field of protein extraction, can solve the problems of difficulty in popularization, harsh requirements, and no production significance, and achieve the effects of simple process, improved utilization rate and high purity

Active Publication Date: 2017-05-31
长睿生物技术(成都)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yields of these methods are relatively ideal, but the requirements are harsh and special equipment is required, so it is difficult to popularize and has no production significance

Method used

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  • Method for extracting albumin and globulin from bovine serum and method for utilizing ox blood

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Embodiment 1

[0055] This embodiment provides a method for extracting albumin and globulin from bovine serum, the process flow of the method is as follows figure 1 shown. Specifically, it includes:

[0056] In the first step, the bovine serum separation step is carried out.

[0057] Take 1L of fresh bovine blood and place it in a container, keep it warm at 37°C for 3 hours, then refrigerate at 4°C for 12 hours, absorb the yellow liquid precipitated from the upper layer to obtain bovine serum, and refrigerate at 4°C for later use .

[0058] The second step is the preprocessing step.

[0059] The above-mentioned bovine serum was taken, centrifuged at 3500r / min for 30min, and the precipitate was discarded to obtain the first supernatant, and an equal volume of 5mM PBS with pH 7.0 was added to the first supernatant, and stirred evenly. Slowly add saturated (NH 4 ) 2 SO 4 solution to (NH 4 ) 2 SO 4 The Saturation is 50%. After stirring evenly, refrigerate and stand at 4° C. for 12 hou...

Embodiment 2

[0074] This example provides a method for extracting albumin and globulin from bovine serum. It includes:

[0075] In the first step, the bovine serum separation step is carried out.

[0076] Take 1L of fresh bovine blood and place it in a container, keep it warm at 35°C for 2 hours, refrigerate at 2°C for 14 hours, absorb the yellow liquid precipitated from the upper layer to obtain bovine serum, and refrigerate at 2°C for later use .

[0077] The second step is the preprocessing step.

[0078] The above-mentioned bovine serum was taken, centrifuged at 3000r / min for 20min, and the precipitate was discarded to obtain the first supernatant, and 0.8 times the volume of 4mM PBS with pH 7.2 was added to the first supernatant, and stirred evenly. Slowly add saturated (NH 4 ) 2 SO 4 solution to (NH 4 ) 2 SO 4 The Saturation is 48%. After stirring evenly, refrigerate and stand at 2°C for 14 hours, and then filter with suction to obtain the first filtrate and the first preci...

Embodiment 3

[0092] This example provides a method for extracting albumin and globulin from bovine serum. It includes:

[0093] In the first step, the bovine serum separation step is carried out.

[0094] Take 1L of fresh bovine blood and place it in a container, keep it warm at 38°C for 4 hours, refrigerate at 8°C for 16 hours, absorb the yellow liquid precipitated from the upper layer to obtain bovine serum, and refrigerate at 8°C for later use .

[0095] The second step is the preprocessing step.

[0096] The above-mentioned bovine serum was taken, centrifuged at 4000r / min for 40min, and the precipitate was discarded to obtain the first supernatant, and an equal volume of 6mM PBS with pH 7.4 was added to the first supernatant, and stirred evenly. Slowly add saturated (NH 4 ) 2 SO 4 solution to (NH 4 ) 2 SO 4 The Saturation is 52%. After stirring evenly, refrigerate and stand at 8°C for 16 hours, and then filter with suction to obtain the first filtrate and the first precipitat...

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Abstract

The invention discloses a method for extracting albumin and globulin from bovine serum and a method for utilizing ox blood and relates to the technical field of protein extraction. According to the method for extracting albumin and globulin from bovine serum provided by the invention, the principle of plasma protein depositing in saline solution in a certain saturability is utilized, (NH4)2SO4 fractional precipitation is adopted and the bovine serum albumin is further purified. The bovine serum albumin is high in purity, is low in endotoxin content and meets the requirement of Chinese Biological Product Regulation. A saturated (NH4)2SO4 fractional repeated salting out method is utilized to extract the bovine serum albumin; the high molecular weight plasma protein is extremely removed; and the purity of the extracted bovine serum albumin is high. The method for extracting albumin and globulin from bovine serum provided by the invention is simple in process; no special device is required; the method is suitable for expanded production; and the method for utilizing ox blood provided by the invention can increase the use ratio of the ox blood.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a method for extracting albumin and globulin from bovine serum and a method for utilizing bovine blood. Background technique [0002] Bovine blood processing technology mainly refers to the process of extracting various proteins from bovine blood to provide added value of bovine blood and improve its utilization. At present, there are many separation and purification methods for bovine serum albumin at home and abroad, such as ion exchange, gel filtration, affinity chromatography, zone electrophoresis, and ultracentrifugation. The yields of these methods are all relatively ideal, but they require harsh conditions and special equipment, so they are difficult to popularize and have no production significance. Contents of the invention [0003] One of the objects of the present invention is to provide a method for extracting albumin and globulin from bovine blood, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K14/47C07K1/14C07K1/30
CPCC07K14/4717C07K14/765
Inventor 贾芳苗刘冬邓飞
Owner 长睿生物技术(成都)有限公司
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