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A method for extracting steviol glycosides in stevia rebaudiana by simulated moving bed chromatography

A technology of simulating moving bed and steviol glycosides, which is applied in the field of purification of natural plant effective components, can solve the problems of large amount of resin and mobile phase, long resin regeneration cycle, and reduced product yield, so as to reduce energy consumption and purification cost, water consumption reduction effect

Active Publication Date: 2019-05-28
UNIV OF SCI & TECH LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In these processes, the water consumption is large during water soaking and extraction, which requires distilled water concentration and high energy consumption; when the flocculant is used to remove impurities, a large amount of precipitation will be generated after adding flocculant to the leachate, although most impurities can be removed, but it will also be adsorbed. A part of steviol glycosides reduces the product yield and produces a large amount of salt at the same time; column chromatography purification is a key step of the process, in addition to removing a small amount of pigment, tannin and other sugars remaining in the stevioside extract, it can also desalt and other impurities , but the amount of resin and mobile phase is large, the utilization rate of resin is low, and the resin needs to be regenerated. The regeneration cycle of desalination and decolorization resin is generally very long, and a large amount of waste water is generated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A method for extracting steviol glycosides in Stevia rebaudiana by simulated moving bed chromatography, the specific separation process is as follows:

[0045]1) Percolation and extraction: crush the leaves of stevia rebaudiana, the particle size of <2mm accounts for 60-90%, put 500g powder into the percolation container, set the leachate outlet under the percolator, and use a small filter screen at the leachate outlet Or filter cloth or cotton to prevent the powder from flowing out. The powder height is 20cm. At room temperature, use 3.5L water to infiltrate and extract, and the cloudy liquid at the beginning can be returned to the diafiltration container for re-diafiltration. This process does not require coarse filtration to directly obtain clarified leachate ;

[0046] 2) Impurity removal by column chromatography: the stationary phase of column chromatography is AB-8, the column specification is: 50 mm in diameter, 300 mm in length, and the loading amount of the lea...

Embodiment 2

[0060] Except the following SMB separation conditions, other conditions and steps are the same as in Example 1.

[0061] SMB separation conditions:

[0062] Mobile phase flow rate: in the refining zone and adsorption zone, the eluent D flow rate Q D 6.3BV per hour, flow rate Q of sample liquid F F 0.76BV per hour, raffinate R flow rate Q R =Q D +Q F ; In the elution band, the eluent P flow rate Q p is 6.3BV per hour, the flow rate of extract E is Q E =Q p ;Switching time T S : 17min;

[0063] The crude product of stevioside obtained by distilling and concentrating the stevioside extract has a yield of 12.2%, and the purity of RA is 68%.

Embodiment 3

[0065] Except the following SMB separation conditions, other conditions and steps are the same as in Example 1.

[0066] SMB separation conditions:

[0067] Mobile phase D flow rate: In the purification zone and adsorption zone, the eluent D flow rate Q D 4.6BV per hour, flow rate Q of sample liquid F F 3.8BV per hour, raffinate R flow rate Q R =Q D +Q F ; In the elution band, the eluent P flow rate Q p is 4.6BV per hour, the flow rate of extract E is Q E =Q p ;Switching time T S : 15.5min;

[0068] The crude product of stevioside obtained by distilling and concentrating the stevioside extract has a yield of 9.7%, and the purity of RA is 67%.

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Abstract

The invention provides a method for extracting steviol glycosides in stevia rebaudiana by simulated moving bed chromatography, which belongs to the purification method of natural plant effective components. In the process of extracting steviol glycosides, column chromatography purification is a key step, but the amount of resin is large, the utilization rate is low, the regeneration cycle is long, and a large amount of waste water is generated, most of which are batch operations. The SMBC separation technology using C18 as the stationary phase in the present invention can purify steviol glycosides from stevioside crude extracts in a large-scale, robust, continuous, automatic and efficient manner, and the stationary phase and mobile phase can be used repeatedly, reducing the cost of purification and belonging to green Environmentally friendly separation process. Combining water leaching and chromatographic impurity removal, water consumption and energy consumption are reduced, flocculation and impurity removal are not required, stationary phase does not need special regeneration, waste water discharge is reduced, and the process is simple.

Description

technical field [0001] The invention relates to a method for purifying effective components of natural plants, in particular to a method for extracting steviol glycosides in stevia rebaudiana by simulated moving bed chromatography. Background technique [0002] Steviol glycosides in stevia rebaudiana are known as "the best natural sweeteners". They are high in sweetness, low in calories, non-toxic and stable. Steviol glycosides have been widely used in food, medicine, health care and beverages in many countries at home and abroad. and other industries. Rebaudioside A (RA) is the monomer with the highest sweetness and the best taste among steviol glycosides. At present, the general process for extracting steviol glycosides from Stevia rebaudiana includes: Stevia rebaudiana water immersion extraction, coarse filtration, flocculant impurity removal, fine filtration, column chromatography purification, concentration drying and crystallization and other processes. In these proc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/256C07H1/08
CPCC07H1/08C07H15/256
Inventor 王绍艳魏伯峰李守江边春阳林怀朋
Owner UNIV OF SCI & TECH LIAONING
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