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A method for extracting alliin from black garlic

An extraction method, alliin technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., to achieve the effect of simple method, convenient and efficient acquisition, and simple and efficient extraction

Active Publication Date: 2018-02-02
江苏福多美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still no related process for extracting alliin from black garlic, and the present invention will fill up the technical gap in this field

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 A kind of method extracting alliin from black garlic

[0035] (1) Raw material processing: mince 100g of black garlic to obtain raw material A;

[0036] (2) Alternate high and low temperature treatment: freeze raw material A at -10°C for 2 hours, then place it at 50°C for 1 hour;

[0037] (3) Repeat step (2) twice to obtain raw material B;

[0038] (4) Extraction: After adding the extraction solution to the raw material B according to the liquid-to-material ratio of 1:1, beat and filter, centrifuge the filtrate at 2000rpm for 20min and collect the supernatant; the extraction solution has a volume concentration of 15 % ethanol aqueous solution, wherein also containing the sodium chloride that mass volume concentration is 2.0%;

[0039] (5) Adsorption separation: After concentrating the supernatant obtained in step (4) under reduced pressure, use HCl solution to adjust the pH value to 1.8, and then use 732-type cation exchange resin for adsorption at a flow r...

Embodiment 2

[0041] Embodiment 2 A method of extracting alliin from black garlic

[0042] (1) Raw material processing: mince 100g of black garlic to obtain raw material A;

[0043] (2) Alternate high and low temperature treatment: freeze raw material A at -2°C for 6 hours, and then place it at 20°C for 3 hours;

[0044] (3), repeat step (2) 4 times to obtain raw material B;

[0045] (4) Extraction: After adding the extraction solution to the raw material B according to the liquid-material ratio of 5:1, beat and filter, centrifuge the filtrate at 5000rpm for 10min and collect the supernatant; the extraction solution has a volume concentration of 5 % ethanol aqueous solution, wherein also containing the sodium chloride that mass volume concentration is 0.5%;

[0046] (5) Adsorption separation: After concentrating the supernatant obtained in step (4) under reduced pressure, use HCl solution to adjust the pH value to 4.5, then use D61 type cation exchange resin for adsorption at a flow rate ...

Embodiment 3

[0048] Embodiment 3 A kind of method extracting alliin from black garlic

[0049] (1) Raw material processing: mince 100g of black garlic to obtain raw material A;

[0050] (2) Alternate high and low temperature treatment: freeze raw material A at -8°C for 3 hours, then place it at 25°C for 2.5 hours;

[0051] (3), repeat step (2) 3 times to obtain raw material B;

[0052] (4) Lixiviation: After adding the leach solution to the raw material B according to the liquid-to-material ratio of 1.5:1, beat and filter, centrifuge the filtrate at 3000rpm for 18min and collect the supernatant; the leach solution has a volume concentration of 7% aqueous ethanol solution, which also contains sodium chloride with a mass volume concentration of 0.8%;

[0053] (5) Adsorption separation: After concentrating the supernatant obtained in step (4) under reduced pressure, use HCl solution to adjust the pH value to 5.5, and then use D001×7 type cation exchange resin for adsorption at a flow rate o...

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Abstract

The invention belongs to the technical field of biology and relates to a method for extracting alliin from black garlic. The method comprises the following steps: (1) raw material processing: mincing the black garlic, so as to obtain a raw material A; (2) high-low temperature alternate treatment; (3) repeated performing of the step (2) for 2-4 times, so as to obtain a raw material B; (4) extraction: adding extraction liquid into the raw material B in a liquid-material ratio of 1:1 to 5:1, carrying out pulping and filtering, centrifuging filtrate, and collecting supernate; (5) adsorption separation: carrying out reduced pressure concentration on the supernate obtained in the step (4), adsorbing the alliin in the supernate by virtue of cation exchange resin, carrying out desorption with ammonia water, and collecting desorption liquid; and (6) regulating the pH value of the desorption liquid, filtering, and concentrating and drying the filtrate, so as to obtain the alliin. The method for extracting alliin from black garlic is simple, efficient, rapid and low in cost and equipment requirement and can be used for conveniently and efficiently acquiring the alliin, and the technical blank for extracting the alliin from the black garlic is filled up.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for extracting alliin from black garlic. Background technique [0002] Black garlic, also known as black garlic, fermented black garlic, is a food made by fermenting fresh raw garlic with skin in a fermentation container. Compared with raw garlic, the nutritional and health functions and medicinal value of black garlic have been greatly improved, such as anti-oxidation, anti-cancer and anti-cancer, sterilization and anti-inflammatory, anti-aging, blood sugar regulation, liver protection, and immune enhancement. Function, treatment of hypertension and cardiovascular and cerebrovascular diseases. In recent years, there have been many studies on the medicinal value of black garlic, especially the anti-cancer and anti-cancer functions of black garlic have attracted more attention from scientific researchers. Studies have shown that black garlic water extract has a significant in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C317/48C07C315/06
CPCC07C315/06C07C317/48
Inventor 张黎明沙如意韩彬曹梦辉张明永
Owner 江苏福多美生物科技有限公司
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