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Method for preparing platelet rich plasma (PRP) and application of PRP

A platelet-rich plasma and platelet technology, applied in the field of clinical medicine, can solve the problems of complicated operation, difficult to apply human blood, undisclosed platelet yield, etc., and achieve the effect of high recovery rate and high concentration

Active Publication Date: 2017-05-31
FIVE DIMENSION BY INCOSC HEALTH MANAGEMENT JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of platelet enrichment in mouse blood is believed to yield concentrations up to 10 10 platelets, but this method does not disclose the yield of platelets, and the method is complicated in operation, and it is difficult to apply to the processing of human blood

Method used

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  • Method for preparing platelet rich plasma (PRP) and application of PRP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Example 1: Extraction of platelet-rich plasma (PRP)

[0118] (1) Collect the subject's whole blood into a container containing anticoagulant, which is a blood collection anticoagulant tube that can be centrifuged. The anticoagulant uses ethylenediaminetetraacetic acid.

[0119] (2) Fully mix the blood and anticoagulant to avoid coagulation. Extract 100 microliters to count platelets.

[0120] (3) Dispense the blood into centrifuge tubes (15 ml).

[0121] (4) Put the centrifuge tube into the centrifuge and centrifuge at 2400rpm for 4 minutes.

[0122] (5) The blood after centrifugation should be divided into three layers: the bottom layer is red blood cells, the top layer is platelet-containing plasma, and the middle layer is Buffy Coat, which contains platelets and white blood cells.

[0123] (6) Use a pipette to extract the uppermost layer of plasma and most of the buffy coat and transfer them to a new centrifuge tube, avoiding extraction of red blood cells as muc...

Embodiment 2

[0131] Example 2: Extraction of platelet-rich plasma (PRP)

[0132] (1) Collect the subject's whole blood into a container containing anticoagulant, which is a blood collection anticoagulant tube that can be centrifuged. Disodium edetate was used as an anticoagulant.

[0133] (2) Fully mix the blood and anticoagulant to avoid coagulation. Extract 100 microliters to count platelets.

[0134] (3) Dispense the blood into centrifuge tubes (15 ml).

[0135] (4) Put the centrifuge tube into the centrifuge and centrifuge at 2400rpm for 4 minutes.

[0136] (5) The blood after centrifugation should be divided into three layers: the bottom layer is red blood cells, the top layer is platelet-containing plasma, and the middle layer is Buffy Coat, which contains platelets and white blood cells.

[0137] (6) Use a pipette to extract the uppermost layer of plasma and most of the buffy coat and transfer them to a new centrifuge tube, avoiding extraction of red blood cells as much as pos...

Embodiment 3

[0145] Example 3: Extraction of platelet-rich plasma (PRP)

[0146] (1) Collect the subject's whole blood into a container containing anticoagulant, which is a blood collection anticoagulant tube that can be centrifuged. Dextrose citrate solution was used as anticoagulant.

[0147] (2) Fully mix the blood and anticoagulant to avoid coagulation. Extract 100 microliters to count platelets.

[0148] (3) Dispense the blood into centrifuge tubes (15 ml).

[0149] (4) Put the centrifuge tube into the centrifuge and centrifuge at 2400rpm for 4 minutes.

[0150] (5) The blood after centrifugation should be divided into three layers: the bottom layer is red blood cells, the top layer is platelet-containing plasma, and the middle layer is Buffy Coat, which contains platelets and white blood cells.

[0151] (6) Use a pipette to extract the uppermost layer of plasma and most of the buffy coat and transfer them to a new centrifuge tube, avoiding extraction of red blood cells as much as...

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Abstract

The invention relates to a method for preparing platelet rich plasma (PRP) and application of the PRP. Specifically, the invention relates to a method for extracting the PRP from blood. The method comprises the steps of (a) placing collected whole blood in a container containing an anticoagulant, and making the blood and the anticoagulant fully mixed; (b) placing the blood mixed with the anticoagulant in a centrifugal tube for the first time of centrifugation, so as to make the blood divided into three layers basically; (c) extracting the uppermost layer and most of the middle layer of blood and transferring the extracted blood to a new centrifugal tube to be evenly mixed, and conducting the second time of centrifugation; and (d) discarding plasma on the upper layer of the centrifugal tube, and making precipitated blood platelets suspend again by means of the rest of plasma, so that the PRP is obtained. The invention further relates to the PRP prepared with the method, and application of the PRP to preparation of health products, for example, the PRP can be applied to the department of orthopaedics, the department of stomatology, the dentofacial surgery, sports medicine and cosmetic medicine.

Description

technical field [0001] The invention belongs to the technical field of clinical medicine, in particular to a method for preparing platelet-rich plasma, and more particularly to a method for obtaining platelet-rich plasma with one or more advantages such as high platelet concentration and high platelet recovery rate. Preparation. The present invention also relates to the platelet-rich plasma PRP prepared by the method of the present invention, and the use of the platelet-rich plasma PRP prepared by the method of the present invention in the preparation of health products. For example, said health products are used in orthopedics, stomatology, maxillofacial surgery, sports medicine and aesthetic medicine. Background technique [0002] Platelet-rich plasma, also commonly referred to as platelet-rich plasma (Platelet Rich Plasma, PRP) in the art, is plasma containing a high volume fraction of platelets obtained by centrifuging human or animal whole blood. Since David R. Knight...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/16A61K35/19B01D17/038A61P19/08A61P1/02A61P21/00A61P17/00
CPCA61K35/16A61K35/19B01D17/0217
Inventor 李诣书尚聪梅俊周丹
Owner FIVE DIMENSION BY INCOSC HEALTH MANAGEMENT JIANGSU
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