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Preparation and application of synbiotics additive used for culturing of stichopus japonicus

A technology of synbiotics and additives, applied in application, additional food elements, animal feed, etc., can solve the problems of restricting the healthy and sustainable development of the sea cucumber industry, destroying the balance of normal flora, reducing production performance, etc., and improving the sea cucumber industry. Breeding quality and efficiency, improving growth performance and non-specific immune response, and promoting animal growth

Inactive Publication Date: 2017-05-31
DALIAN SEM BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the transformation of the sea cucumber industry to a high-density intensive mode, the health and disease problems of sea cucumber farming have become increasingly prominent, and diseases such as rot skin syndrome have occurred frequently, resulting in reduced production performance and seriously restricting the healthy and sustainable development of the sea cucumber industry.
Although the use of antibiotics and chemical drugs to prevent and control aquatic animal diseases has certain effects, it will lead to many disadvantages such as bacterial resistance, drug residues, and damage to the normal flora balance between animals and the environment.

Method used

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  • Preparation and application of synbiotics additive used for culturing of stichopus japonicus
  • Preparation and application of synbiotics additive used for culturing of stichopus japonicus

Examples

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Embodiment 1

[0036] 1. Preparation of marine synbiotic additives

[0037] Step 1: Ferment and cultivate Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum respectively in the improved MRS marine culture medium. Lactobacillus plantarum fermentation liquid, adjust three kinds of probiotic fermentation liquid to 1×10 10 CFU / mL, the three bacterial solutions were mixed to obtain a mixed bacterial solution;

[0038] Step 2: Mix the above-mentioned three probiotic mixed bacterial liquids with prebiotics and skim milk powder, so that the mass concentration of the prebiotics is 5%, and the mass concentration of the skim milk powder protective agent is 10%;

[0039] The ratio of chitosan oligosaccharide and inulin in the prebiotic is 1:3;

[0040] Step 3: Mix the step 2 solution with the mineral carrier zeolite powder, the volume to mass ratio is 1:0.25ml / g;

[0041] Step 4: Vacuum freeze-dry the mixture prepared in Step 3 for 24 hours to make freeze-dried powder, which is the...

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Abstract

The invention provides preparation and application of synbiotics additive used for culturing of stichopus japonicus. The additive is composed of bacillus licheniformis, bacillus subtilis and plant lactic acid bacillus; prebiotics is composed of alginate oligosaccharide and inulin; other auxiliary ingredients comprise a thallus protective agent and a mineral substance carrier. The invention further provides a preparation method of the synbiotics additive. Probiotics liquid is taken, the prebiotics and the thallus protective agent are added, and the mixture is mixed with the mineral substance carrier; the prepared mixture is subjected to vacuum freeze-drying, and freeze-dried powder is prepared and is namely the synbiotics additive used for culturing of the stichopus japonicus. The invention further provides application of the prebiotics additive for culturing of stichopus japonicus. Probiotics and synbiotics are efficiently and scientifically compatible, and achieve a synergistic effect and the effect that 1+1>2; by the utilization of the synbiotics additive, the dual probiotic effects of the probiotics and prebiotics can be achieved at the same time, the growth performance and non-specific immunity response of the stichopus japonicus can be obviously improved, and the healthy growth of animals can be better promoted.

Description

technical field [0001] The invention relates to a method for developing a synbiotic additive for sea cucumber cultivation and its application in sea cucumber cultivation. Background technique [0002] Sea cucumber is one of the best quality among more than 20 kinds of edible sea cucumbers in China. It ranks first among the eight seafood treasures and has extremely high nutritional and economic value. In the past ten years, the sea cucumber breeding industry has developed rapidly. In 2013, the national sea cucumber production exceeded 13×10 4 tons, with an output value of more than 30 billion yuan, which is the highest in the output value and profit of a single species in the aquaculture industry. Its development speed and comprehensive economic benefits are unmatched by other economic aquatic species. With the transformation of the sea cucumber industry to a high-density intensive mode, the health and disease problems of sea cucumber farming have become increasingly promine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/18A23K20/163A23K10/30A23K10/20A23K20/28
Inventor 徐永平李淑英王熙涛
Owner DALIAN SEM BIOLOGICAL ENG TECH
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