Preparation and application of synbiotics additive used for culturing of stichopus japonicus
A technology of synbiotics and additives, applied in application, additional food elements, animal feed, etc., can solve the problems of restricting the healthy and sustainable development of the sea cucumber industry, destroying the balance of normal flora, reducing production performance, etc., and improving the sea cucumber industry. Breeding quality and efficiency, improving growth performance and non-specific immune response, and promoting animal growth
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[0036] 1. Preparation of marine synbiotic additives
[0037] Step 1: Ferment and cultivate Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum respectively in the improved MRS marine culture medium. Lactobacillus plantarum fermentation liquid, adjust three kinds of probiotic fermentation liquid to 1×10 10 CFU / mL, the three bacterial solutions were mixed to obtain a mixed bacterial solution;
[0038] Step 2: Mix the above-mentioned three probiotic mixed bacterial liquids with prebiotics and skim milk powder, so that the mass concentration of the prebiotics is 5%, and the mass concentration of the skim milk powder protective agent is 10%;
[0039] The ratio of chitosan oligosaccharide and inulin in the prebiotic is 1:3;
[0040] Step 3: Mix the step 2 solution with the mineral carrier zeolite powder, the volume to mass ratio is 1:0.25ml / g;
[0041] Step 4: Vacuum freeze-dry the mixture prepared in Step 3 for 24 hours to make freeze-dried powder, which is the...
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