Preparation method for fermenting and producing bacteriocin rice feed rich in taurine
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A technology of taurine and rice, applied in the field of agriculture and animal husbandry, can solve problems such as slow growth of fish, achieve normal visual function, promote full absorption, and regulate nerve conduction.
Active Publication Date: 2019-11-15
CHANGSHA UNIVERSITY
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Problems solved by technology
Studies have shown that plant-based protein baits lacking taurine can lead to slow growth and some pathological phenomena in fish, such as green liver syndrome and hemolytic anemia
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Embodiment 1
[0017] Embodiment 1: For fermenting and producing bacteriocin rich in taurine, the rice feed comprises the following raw materials in parts by mass: rice: 40 parts, soybean meal: 20 parts, bran: 20 parts, cysteine: 5 parts, Methionine 5 parts.
[0018] Be used to ferment and produce the bacteriocin rice feed preparation step that is rich in taurine: step 1: the preparation of potato culture medium: be potato by mass ratio: the ratio of water=1:6 prepares potato juice, after adding potato to water, Boil for 25 minutes, filter and remove slag to obtain potato juice, then add 2% glucose by volume of potato juice to make potato medium liquid;
[0019] Step 2. Activation and cultivation of strains: Add 20mL of the potato medium liquid prepared in step 1 to the activated strains of Bacillus subtilis YS-45, Saccharomyces S-78 and Aspergillus niger PL-39 respectively to make a bacterial suspension , and then the bacteria suspension, respectively, under 250r / min shaker, 32 ℃ under the...
Embodiment 2
[0023] Embodiment 2: For fermenting and producing bacteriocin rich in taurine, the rice feed comprises the following raw materials in parts by mass: rice: 42 parts, soybean meal: 20 parts, bran: 20 parts, cysteine: 4 parts, Methionine 4 parts.
[0024] Be used to ferment and produce the bacteriocin rice feed preparation step that is rich in taurine: step 1, the preparation of potato culture medium: be potato by mass ratio: the ratio of water=1:4 prepares potato juice, after adding potato to water, Boil for 35 minutes, filter to remove slag to obtain potato juice, then add 2% glucose by volume of potato juice to make potato medium liquid;
[0025] Step 2. Activation and cultivation of strains: Add 15ml of the potato medium liquid prepared in step 1 to the activated strains of Bacillus subtilis YS-45, Saccharomyces S-78, and Aspergillus niger PL-39 respectively to make a bacterial suspension. Then, the bacterial suspension was expanded and cultivated under 250r / min shaker at 30...
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Abstract
The present invention discloses a preparation method of a taurine enriched microorganism peptide rice feed produced by a fermentation and relates to the technical field of agriculture and animal husbandry. The preparation method is as follows: a potato culture medium is prepared and microorganism strains are activated and cultured; rice, bran, soybean meal, cysteine and methionine are weighed, the weighed materials are stirred and mixed evenly, the mixture is subjected to a high temperature steam sterilization, the sterilized mixture is cooled to obtain solid fermentation products, a seed microorganism suspension is inoculated into the sterilized solid fermentation products, then water is added, the materials are stirred evenly, and then the stirred materials are cultured in a solid state fermentation tank to obtain the taurine enriched microorganism peptide rice feed. Yeast S-78 converts starch into monosaccharide to provide carbon sources for the fermentation. Aspergillus niger PL-39 produces protease, amylase, pectinase, and organic acids such as citric acids and gluconic acids. The three microorganisms react together to produce various enzymes, degrade rice, soybean meal and bran in the fermentation and produces the taurine enriched microorganism peptide rice feed by combing the cysteine and methionine.
Description
Technical field: [0001] The invention relates to a preparation method for fermenting and producing bacteriocin rice feed rich in taurine, and belongs to the technical field of agriculture and animal husbandry. Background technique: [0002] With the rapid development of my country's feed industry, the prices of conventional protein raw materials such as soybean meal and fish meal continue to rise, the cost of feed increases sharply, and the income of breeding continues to decrease. How to reasonably and effectively use unconventional protein feed resources to replace conventional protein feeds such as soybean meal or fish meal to reduce feed costs has become a market concern. As a major rice producing province, our province produced 11.9845 million tons of rice in 2013, a year-on-year increase of 1.37%. In addition to supplying people's daily dietary needs, these rices still have a large proportion of rice used as raw materials for industrial fermentation and starch sugar p...
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