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Method for increasing content of mogroside V

A mogroside and Luo Han Guo technology, applied in the directions of horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve problems such as restricting the healthy development of the Luo Han Guo industry, achieve uniform distribution of nutrients, maintain nutrients, and increase content Effect

Inactive Publication Date: 2017-05-31
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, glucoside V only exists in the pulp which accounts for less than 15% of the fruit weight, and the content is only about 1%, which seriously restricts the healthy development of the Luo Han Guo industry
There is no report in the prior art that methyl jasmonate increases the content of mogroside V

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The invention provides a method for increasing the content of mogroside V, comprising the following steps:

[0022] Step 1, dissolving methyl jasmonate in ethanol aqueous solution with a volume fraction of 20%, is prepared into an ethanol mother liquor of methyl jasmonate with a concentration of 50mmol / L, for subsequent use; dissolving methyl jasmonate in ethanol is prepared into Concentration is the methyl jasmonate solution of 50 μ mol / L, standby;

[0023] Step 2, inoculate the tissue cultured seedlings of Luo Han Guo in solid medium, and cultivate them for 30 days under the conditions of relative humidity of 60%, light intensity of 1400 lux, light time of 8h / d, and culture temperature of 21°C, wherein the solid medium The production method is as follows: when making a solid medium, add the ethanol mother liquor of methyl jasmonate prepared in step 1 to the solid medium until the final concentration of methyl jasmonate is 50 μmol / L, and then add the solid medium Ster...

Embodiment 2

[0030] The invention provides a method for increasing the content of mogroside V, comprising the following steps:

[0031] Step 1, dissolving methyl jasmonate in ethanol aqueous solution with a volume fraction of 20%, is prepared into an ethanol mother liquor of methyl jasmonate with a concentration of 50mmol / L, for subsequent use; dissolving methyl jasmonate in ethanol is prepared into Concentration is the methyl jasmonate solution of 400 μ mol / L, standby;

[0032]Step 2, inoculate the tissue cultured seedlings of Luo Han Guo in solid medium, and cultivate them for 30 days under the conditions of relative humidity of 66%, light intensity of 1400 lux, light time of 8h / d, and culture temperature of 25°C, wherein the solid medium The production method is as follows: when making a solid medium, add the ethanol mother liquor of methyl jasmonate prepared in step 1 to the solid medium until the final concentration of methyl jasmonate is 400 μmol / L, and then add the solid medium Ste...

Embodiment 3

[0039] The invention provides a method for increasing the content of mogroside V, comprising the following steps:

[0040] Step 1, dissolving methyl jasmonate in ethanol aqueous solution with a volume fraction of 20%, is prepared into an ethanol mother liquor of methyl jasmonate with a concentration of 50mmol / L, for subsequent use; dissolving methyl jasmonate in ethanol is prepared into Concentration is the methyl jasmonate solution of 220 μ mol / L, standby;

[0041] Step 2, inoculate the tissue cultured seedlings of Luo Han Guo in solid medium, and cultivate them for 30 days under the conditions of relative humidity of 63%, light intensity of 1400 lux, light time of 8h / d, and culture temperature of 23°C, wherein the solid medium The production method is: when making the solid medium, add the ethanol mother liquor of methyl jasmonate prepared in step 1 to the solid medium until the final concentration of methyl jasmonate is 220 μmol / L, and then add the solid medium Sterilize, ...

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Abstract

The invention discloses a method for increasing the content of mogroside V. The method comprises the following steps: step I, dissolving jasmonic acid methyl ester into an ethanol solution of which the volume percentage is 20%, and preparing a 50mmol / L ethanol solution of the jasmonic acid methyl ester; dissolving the jasmonic acid methyl ester into ethanol, and preparing a 50-400[mu] mol / L jasmonic acid methyl ester solution; step II, inoculating a momordica grosvenori tissue culture seedling into a solid culture medium, wherein ethanol mother liquor of the jasmonic acid methyl ester is added into the solid culture medium when the solid culture medium is prepared, and thus the final concentration of the jasmonic acid methyl ester is 50-400[mu] mol / L; step III, transplanting the tissue culture seedling into a field, 50-60 days after pollination of momordica grosvenori, spraying the jasmonic acid methyl ester solution once in the morning, at noon and at night every day till water drops off from leaves, and continuously spraying for 10 days. The method is capable of effectively increasing the yield of the mogroside V and has the characteristics of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or residue in products and the like, and large-scale production of the mogroside V can be met.

Description

technical field [0001] The invention relates to a planting method of Luo Han Guo. More specifically, the present invention relates to a method for increasing the content of mogroside V. Background technique [0002] Luo Han Guo is a precious medicinal and sweet plant unique to China. Its fruit is cool in nature and sweet in taste, and has the functions of clearing away heat and moistening lungs, relieving throat and opening sound, laxative and anticancer. The active ingredient glucoside V is one of the strongest non-sugar sweeteners in the world. It is 300-400 times higher than sugar and is widely used in food, health products and medicines. It is an ideal sugar substitute for diabetics, obese people and hypertensive patients. However, sweet glycoside V only exists in the pulp which accounts for less than 15% of the fruit weight, and the content is only about 1%, which seriously restricts the healthy development of the Luo Han Guo industry. [0003] As a plant signal indu...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/02A01G7/06
CPCA01G7/06A01H1/02A01H4/001A01H4/008
Inventor 韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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