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Tissue culture quick propagation method of Polygonatum kingianum

A technique of tissue culture rapid propagation and Rhizoma Polygonatum, which is applied in the field of plant tissue culture, can solve the problems of uneconomical use, large amount of rhizomes, and low germination rate of seeds, achieve good social and economic benefits, increase the speed of rapid propagation, shorten The effect of time and cost

Inactive Publication Date: 2017-05-31
宣威市绿隆农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because Polygonatum seeds are difficult to collect, the seed germination rate is low, and the seed propagation time is long
It is unfavorable for the artificial large-scale plantation of Rhizoma Polygonatum, so in the artificial cultivation of Rhizoma Polygonatum at present, propagation mainly relies on the vegetative propagation of rhizome, and this method propagation coefficient is low, the consumption of rhizome is big, both uneconomical, has limited the output potential of Rhizoma Rhizoma Polygonatum again. Easy to grow and manage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The concrete steps of this embodiment are as follows:

[0015] ① Selection and treatment of exogenous bodies: select mature, fresh, and pest-free rhizomes of Rhizome Polygonatum, cut the rhizomes with buds with a knife and soak them in an aqueous solution of detergent, wash them with running water after soaking for 1 min, and then use Soak in 70% alcohol for 1 to 2 minutes, rinse twice with sterile water, and finally put it in 0.1% mercuric chloride for 3 times, soaking for 5 minutes each time. After soaking, rinse with sterile water for 2 to 3 times. The mercuric chloride contains 0.02% Tuwen 20 by mass fraction. After rinsing, dry the sterile water with sterile filter paper and cut off the wound with a knife. The necrotic part can be inoculated;

[0016] ②Induction culture: inoculate the rhizomes after the disinfection treatment in step ① on the induction medium for induction culture. Cultivate them under the condition that the culture temperature is 23°C, the light ...

Embodiment 2

[0023] The concrete steps of this embodiment are as follows:

[0024]① Selection and processing of exogenous bodies: select mature, fresh, and pest-free rhizomes of Rhizoma Polygonatum, cut the rhizomes with buds with a knife and soak them in an aqueous solution of detergent, clean them with running water after soaking for 2 minutes, and then wash them with volume Soak in alcohol with a fraction of 72.5% for 2 minutes, rinse repeatedly with sterile water for 3 times, and finally put it into mercury chloride with a mass fraction of 0.2%. It must be washed with sterile water for 2 to 3 times, and then blotted dry with sterile filter paper, and the necrotic part of the wound can be cut off with a knife to inoculate. The mass fraction of mercuric chloride is 0.06 % soil temperature 20;

[0025] ②Induction culture: inoculate the rhizomes after the disinfection treatment in step ① on the induction medium for induction culture. Cultivate them under the condition that the culture tem...

Embodiment 3

[0032] The concrete steps of this embodiment are as follows:

[0033] ① Selection and processing of exogenous bodies: select mature, fresh, and pest-free rhizomes of Rhizoma Polygonatum, cut the rhizomes with buds with a knife and soak them in an aqueous solution of detergent, clean them with running water after soaking for 3 minutes, and then wash them with volume Soak in alcohol with a fraction of 75% for 2 minutes, rinse repeatedly with sterile water for 3 times, and finally put it into mercuric chloride with a mass fraction of 0.3% for 5 times, soaking for 3 minutes each time, after each soaking It must be rinsed with sterile water for 2 to 3 times, and then blotted dry with sterile filter paper. The necrotic part of the wound can be cut off with a knife to inoculate. The mercury chloride contains a mass fraction of 0.1 % soil temperature 20;

[0034] ②Induction culture: inoculate the rhizomes after the disinfection treatment in step ① on the induction medium for inductio...

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PUM

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Abstract

The invention discloses a tissue culture quick propagation method of Polygonatum kingianum. The method comprises the following steps: explant selection and treatment, induced culture, successive transfer culture, enrichment culture, rooting culture, and acclimatization and transplant. The induced culture medium is composed of MS, 1.0-3.0mg / L6-BA, 0.5-1.0mg / L 2,4-D, 1.0-2.0mg / L gibberellin, 5.0-10.0g / L agar and 20.0-30.0g / L sucrose. The rooting culture medium is composed of 1 / 2MS, 0.2-1.0mg / L NAA, 0.2-0.6mg / L IBA, 0.2-0.5% of activated carbon, 5-20% of banana, 6.0-8.0g / L agar and 20.0-25.0g / L sucrose. The method effectively reduces the seed / seedling culture time and cost, increases the quick propagation speed, effectively enhances the Polygonatum sibiricum propagation coefficient, and can well keep the hereditary stability of Polygonatum sibiricum; and the survival percent after transplant can reach 98% or above, thereby creating favorable social benefits and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Polygonatum yunnanensis. Background technique [0002] Polygonatum Polygonatum is the rhizome of Polygonatum Polygonatum, Polygonatum Dianensis or Polygonatum Polyflora, a perennial herb of the Liliaceae family. Modern studies have shown that Polygonatum contains niacin, aldehydes, liquid temperament, starch, diaminobutyric acid, Polygonatum polysaccharides and oligosaccharides, etc., which have the effects of anesthesia and lowering animal blood pressure, and have a positive effect on hyperglycemia caused by adrenaline. It also has a good effect on improving kidney function. Polygonatum is also a good antibacterial medicine blindly, which has certain inhibitory effects on Salmonella typhi, Mycobacterium tuberculosis, Staphylococcus aureus and dermatophytes. In recent years, sealwort has been widely u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨光早陶春艳
Owner 宣威市绿隆农业有限公司
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